Inpp4b (NM_001297593) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E130107I17Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229070 representing NM_001297593 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGAGGACATTCAGTTTACCAGCATCCAGAAGATTCCGAACGAGCCACAGTTGGAATTCATTCT CGGGAACAAAAGACCCACTGTTCCTAACGGGTGTCACATTCCCACCGGACTACCCCATCTATGAAGAGAC AAGAATAAAACTGACAGTCTACGATGTCAAGGATAAGTCTCATGATACTATTCGAACCAGTGTCCTTCCG GAACACAAGGATCCCCTGCCAGAAGTTGCGAGAAGCTTCCTGGGCTGTGCCAGTTTTAAAGTTGGGGAGC TGCTGAAGTCCAAGGAGCAGCTGCTGTCCCTGAGCCTGAGAACCTCAGATGGTGGCAAAGTGGTCGGCAC CATAGAAGTCAGCCTCGTGAAGATGGGAGAGATTGAGGATGGGGACACCGACCACATCACAACAGATGTG CAGGGACAAAAGTGTGCACTAATGTATGAATCCACAGCACCAGAAAGTCTGAGCGGGAAGGAAAACTTAC CTTTTATGAATGCAGTCTTAAGGAATCCTGTGTGTAAGCTGTATAGATTCCCCACATCTGACAATAAGTG GATGCGAATCCGTGAGCAGATGTCAGAAAGCATCCTTTCTTTTCATATTCCTAAGGAATTGATTTCCCTT CACATAAAAGAAGACTTATGTAGAAACCAGGAGTTAAAAGAACTTGGTGACCTTTCCCCACACTGGGACA ACCTACGGAAAAATGTCCTTTCTCACTGTGATCAAATGGTGACTATGTACCAAGACATTCTGACAGAACT TTCCAAAGAAACAGGGTCCTCTTTCAAATCCAGCAGCAGCAAAGGAGAGAAAACATTAGAATTTGTTCCA GTAAATCTACACTTGCAACGGATGCAGGTGCACAGCCCTCACCTGAAAGATGCTCTCTATGATGTCATCA CTGTGGGGGCCCCAGCTGCCCATTTTCAGGGATTCAAGAACGGTGGTCTTCGTAAACTCCTCCATAGGTT TGAGACAGAGAGAAGGAACACCGGCTACCAGTTTATTTACTATTCACCTGAGAACACAGCGAAGGCAAAG GAAGTTCTCAGCAGCATCAATCAGCTACAACCTCTCATAGCAACTCATGCAGACCTGCTGCTAACCTCTG CCAGCCAGCGTTCTCCAGACAGCCTGAAGAGCTCTTTAAAGCTGCTTTCAGAAAAAACTGAGCTTTTTGT CCATGCCTTCAAGGACCAGCTGGTCAGGAGTGCTCTTTTAGCTCTCTACACTGCAAGGCCAGGAGGCATC CTTAAGAAGCCACCCTCTCCTAATGTCAGCACAGAGGAGAAAAGTACTCAGCATGATACCCCACAGCTTA GAAGACAAGACTCCATACCACATCATTCTGACTATGATGAAGAAGAGTGGGATAGGGTGTGGGCCAACGT GGGGAAGAGCCTGAACTGCATTATCGCTAAAGTAGACAAACTGATCGAGAGAGACAGTCACAACGAAGAA GGTGCTGGAGGCAGCAGTAGCAAGGATGGAGAGGCAGACCATACCCTGGAAGATTCCATCACATCTCACC CACGTGAGGACTGGTATGAACAGCTGCACCCACTCATCCTTACCTTGAAGGAGTGCATGGGAGAAGTGGT GAACCGAGCAAAGCAATCCCTGACATTTGTACTGCTTCAGGAACTGGCCTACAGTCTGCCCCAGTGTTTG ATGCTGACTCTGAGAAGAGACATTGTCTTCAGTCAGGCGCTTGCTGGATTGGTTTGTGGTTTTATCATCA AATTACATACAAGTTTGCATGACCCTGGCTTCCTACAGCAGCTTCATACAGTGGGCTTGATAGTACAATA CGAAGGACTGCTAAGTACTTACAGTGATGAAATTGGAATGCTGGAGGACATGGCAGTCGGCATTTCAGAT TTGAGGAAAGTTGCATTTAAAATAACTGAAGCCACGTCCAATGATGTCCTTCCTGTTCTCACGGGGAGAC GGGAACACTATGTGGTGGAGGTCAAGCTTCCAGCTACAGTATTTGAGTCGCTCCCACTGCAGATTAAAGA AGGCCAGTTGCTTCATGTGTATCCCGTTCTGTTTAATGTCGGAATCAATGAGCAGCAGACTCTGGCTGAG AGGTTTGGAGATGTTTCTTTGCAAGAAAGTATTAATCAAGAAAATTTTGAACTTGTACAAGAATATTATT CAATATTTATGGAAAAGATGCCTCCTGATTATATTTCACATTTTCAAGAACAAACTGATTTAAAAGGACT GTTGGACAATCTCCACCAAAATATTCAAGCCAAAAAAAGAAAGAATGTAGAAATCATGTGGCTGGCAGCA ACGATTTGTCGGAAACTCAATGGCATTCGTTTTACCTGCTGTAAAAGTGCCAAAGACAGGACATCGATGT CGGTGACACTGGAACAGTGCTCCATCCTGAGAGACGAGCATCAACTGCACAAAGACTTTTTCATCAGAGC TTTAGATTGCATGAGAAGAGAAGGATGCCGCATAGAGAATGTACTGAAGAATATCAAATGCAGGAGGTAT GCTTTCAACATGCTACAGCTGATGGCTTTCCCCAAGTGCTACAGACCTCCAGAAGGCACTTATGGAAAAG CTGACACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001297593
Insert Size	2601 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297593.1, NP_001284522.1
RefSeq Size	8248 bp
RefSeq ORF	2601 bp
Locus ID	234515
Gene Summary	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate (By similarity). Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (By similarity). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate internal exon and uses an alternate last exon compared to variant 1. The resulting isoform (3) has shorter and distinct N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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