Prkd2 (NM_001252458) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI325941; PKD2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229094 representing NM_001252458 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCCCCCTCCCATCCCGCAGGACTCCCCGGCTCTCCGGGGCCCGGGTCTCCTCCGCCCCCCG GTGGCTTGGATCTCCAGTCGCCGCCACCTCTGCTGCCTCAGATCCCGGCTCCGGGTTCGGGGGTCTCCTT CCATATCCAGATCGGATTGACCCGAGAGTTCGTGCTGTTGCCGGCTGCCTCAGAGTTGGCTCATGTGAAG CAACTAGCCTGTTCTATCGTGGACCAGAAGTTCCCAGAGTGTGGCTTCTACGGCCTGTACGACAAGATCC TGCTCTTCAAACATGACCCAACATCAGCCAACCTCCTGCAGCTGGTGCGATCAGCTGCAGATATCCAGGA GGGTGACCTGGTGGAGGTGGTTCTGTCGGCCTCGGCCACCTTCGAGGATTTCCAGATCCGACCGCATGCT CTCACTGTGCACTCGTACCGCGCTCCCGCCTTCTGTGACCACTGCGGGGAGATGCTCTTCGGCCTCGTGC GCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAATTACCACAAACGCTGCGCCTTCAGCATCCCGAACAA CTGCAGTGGTGCTCGAAAGCGCCGCCTGTCATCCACGTCTCTGGCCAGTGGCCATTCTGTGCGCCTTGGC AGCTCCGAGTCCTTACCCTGCACCGCCGAAGAGCTGAGCCGTAGCACCACCGACCTCCTTCCTCGCCGAC CACCCTCGTCGTCGTCCTCCTCCTCTTCCTCTTCCTTCTACACAGGCCGACCCATTGAGTTGGACAAGAT GCTGATGTCCAAGGTCAAGGTGCCACACACCTTCCTTATACATAGCTACACACGGCCCACCGTTTGCCAG GCTTGCAAGAAACTGCTCAAGGGTCTATTCCGCCAGGGCCTGCAGTGCAAAGACTGCAAGTTTAACTGTC ACAAACGCTGTGCTACCCGCGTCCCTAACGACTGCCTGGGGGAGGCGCTCATCAATGGAGACGTGCCGAT GGAGGAAGCCGCTGATTACAGTGAGGCTGATAAGAGCTCCATCTCAGATGAGTTGGAGGATTCTGGTGTC ATCCCCGGCTCCCACTCAGAGAGTGCTCTCCATGCCAGTGAGGAAGAGGAAGGCGAGGGACACAAGGCCC AGAGCTCGCTGGGATACATCCCCCTGATGCGCGTGGTACAGTCTGTGCGACACACGACCCGGAAATCCAG CACCACCCTGCGGGAGGGCTGGGTGGTCCATTACAGCAACAAGGACACACTGAGGAAACGGCACTACTGG CGTCTAGACTGTAAATGCATTACCCTCTTCCAGAACAACACGACCAACAGATACTACAAGGAAATCCCGC TGTCTGAAATCCTTGCGGTGGAACCGGCTCAGAACTTCAGCCTCGTACCCCCGGGCACCAACCCACACTG CTTCGAGATCATCACGGCCAATGTCACCTACTTTGTGGGCGAAACCCCTGGCGGGGCCCCGGGAGGGCCA AGTGGACAGGGAACAGAGGCCGTTCGGGGTTGGGAGACAGCCATCCGCCAGGCACTGATGCCCGTTATCC TCCAAGATGCGCCCAGTGCCCCGGGGCACACACCGCACAGACAAGCGTCTCTGAGTATCTCCGTGTCCAA CAGTCAGATCCAAGAGAATGTGGATATCGCCACTGTCTACCAGATCTTCCCAGATGAGGTGCTGGGCTCT GGGCAGTTTGGAGTGGTTTATGGAGGAAAACACAGGAAGACTGGCAGAGACGTTGCAGTAAAGGTCATTG ACAAACTGCGCTTCCCCACCAAGCAGGAGAGCCAGCTCAGGAATGAAGTGGCCATTCTCCAGAGTCTACG GCACCCCGGGATTGTGAACCTCGAGTGCATGTTTGAGACTCCTGAGAAGGTATTCGTGGTGATGGAGAAA CTGCACGGGGACATGTTGGAGATGATCCTCTCCAGTGAGAAGGGCCGGCTTCCTGAGCGTCTCACCAAGT TCCTTATCACGCAGATCCTGGTGGCGCTGAGACACCTGCACTTCAAGAACATCGTCCACTGTGACTTGAA ACCAGAGAATGTGTTACTGGCATCAGCCGATCCGTTTCCTCAGGTGAAGCTCTGTGACTTTGGCTTCGCT CGCATCATCGGCGAGAAGTCCTTCCGGCGCTCAGTGGTGGGCACGCCTGCCTACCTGGCACCCGAGGTAC TGCTCAACCAGGGTTACAACCGCTCGCTGGACATGTGGTCCGTGGGTGTGATCATGTACGTGAGCCTTAG TGGCACGTTCCCCTTCAATGAGGACGAGGACATCAATGACCAGATACAGAACGCGGCCTTTATGTACCCG GCCAGCCCCTGGAGCCACATCTCATCTGGAGCCATCGACCTCATCAACAACCTGTTGCAGGTGAAGATGC GCAAGCGCTACAGCGTGGACAAGTCTCTCAGCCACCCATGGTTACAGGAGTACCAGACGTGGCTTGACCT CCGAGAGCTAGAGGGAAAGATGGGCGAGCGATATATCACGCACGAGAGCGACGACGCACGCTGGGATCAG TTTGTGGCAGAGCGCCATGGGACTCCTGCAGAAGGGGACCTGGGTGGCGCCTGTCTGCCGCAGGACCACG AGATGCAGGGGCTGGCTGAGCGCATCAGCATCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252458
Insert Size	2628 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252458.1, NP_001239387.1
RefSeq Size	3340 bp
RefSeq ORF	2628 bp
Locus ID	101540
UniProt ID	Q8BZ03
Gene Summary	Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion (PubMed:17226786, PubMed:20819079). May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression (PubMed:17226786). In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-718 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B (By similarity). In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77 (By similarity). Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens (PubMed:20819079). In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway (By similarity). During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors (By similarity). In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane (By similarity). Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis (By similarity). In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN (By similarity). Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has a different 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

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