Brd8 (NM_001289607) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610007E11Rik; 4432404P07Rik; SMAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229104 representing NM_001289607 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGGGACAGGCAAGCACAAGCTGCTGAGTACTGGCCCAACAGAGCCATGGTCCATCCGAGAGA AGTTGTGTTTAGCATCTTCTGTCATGAGGAGTGGGGATCAGAACTGGGTATCAGTTAGCAGAGCAATCAA GCCCTTTGCAGAACCTGGCCGGCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCTCAGTATTCTGAG CTCTTAGAGACTACTGAGACTCCAAAACGGAAACGGGGTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAG ATGTTATTGTTCGCAAACTGACTGCTGAGAGAGTTGAAGAACTGAAGAAAGTCATAAAGGAGACACAGGA GAGATACAGGCGTCTGAAAAGAGATGCAGAACTAATCCAAGCCGGGCACATGGACAGCAGACTGGATGAG CTCTGCAATGACATTGCAATGAAAAAGAAATTGGAAGAGGAAGAGGCTGAAGTAAAAAGGAAGGCCACCG ATGCTGCGTACCAGGCGCGACAAGCAGTGAAAACGCCTCCTCGAAGGTTACCGACTGTGATGGTCCGCTC TCCTGTAGACTCTGCCTCCCCAGGAGGTGATTACCCACTTGGAGACTTGACTCCAACCACTATGGAAGAG GCTACCTCTGGGGTAACCCCTGGGACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACA CCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAATAACAGATGATTCACCCCAGAAAAA GATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC CTGCCTACTAGCCCCAGACTGGTGAATGAGAGTGAAATGCCTGTACCCCCTGGCCATCTGAACAGCACAG GGGTTCTCTTGGAGGTAGGAGGGGTTCTTCCCATGATACATGGTGGGGAGATACAGCCAACAACCAGTGC TGTGGCGGCCTCCCCGGCTGCCTCAGTGAGCCAGCCTGAGCCCTGTGTACCCCTGGAAGCTGTGGGGGAT CCACATACTGTGACTGTTTCCATGGATAGCAATGAAATCTCCATGATCATTAATTCCATCAAAGAAGAGT GTTTCCGCTCAGGGGTAGCAGAGGCTCCTGGTGGATCAAAGGCTCCAAGCATAGATGGGAAGGAAGACTT GGATCTAGCGGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGTTGGACTTTGAAACAGTTGGA GACATCATTGCCATCATTGAGGACAAGGTGGATGATCACCCTGAAGTGCTGGATGTGGCAGCGGTAGAAG CGGCGTTATCGTTCTGTGAAGAGAATGATGACCCTCAGTCCCTGCCTGGCCCCTGGGAGCACCCTATCCA GCAGGAGCGCGACAAGCCAGTACCTCTGCCAGCACCAGAGATGACAGTCAAACAAGAGAGGCTAGACTTC GAGGAATCAGAAAACAAAGGGCTCCATGACCTGGTGGACATCAGGGATTCCGGTGTTGAGATTAAGGTGG AACCCACAGAGCCAGAGCCAGGCATGTCTGGGGCTGAGATAGTAGCTGGAGTTGGTCCAGTTCCAAGTAT GGAGCCACCAGAACTCAGGAGTCAAGACTCAGATGAAGAACCTAGAAGTTCTGCAGCTGGAGACATTGGT GAGGCAGATGGTTCCAGTGGGAAAGGCGATGAGAGGCCACTTTCAGCTGTGAAGACAGAGGCATCCCCTG AGAGCATGTTGTCTCCATCACATGGCTCAAATCTTATTGAAGATCCTTTAGAGGCAGAGACTCAACACAA GTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCA GGTGACGATGAGGAAGAAGATGGAGTCAGTGAAGCAGCTAGCCTAGAGGAGCCTAAGGAAGAGGATCAAG GAGAAGGCTATTTGTCTGAGATGGATAATGAGCCCCCTGTGAGTGAGAGTGATGATGGCTTTAGTATACA TAACGCCACACTGCAGTCACACACTCTGGCAGACTCCATCCCAAGCAGCCCTGCCTCCTCCCAGTTTTCC GTGTGCAGTGAAGATCAAGAAGCAATTCAGGCTCAGAAAATATGGAAGAAAGCCATCATGCTTGTATGGA GGGCTGCAGCAAATCATAGGTATGCCAATGTGTTCCTGCAACCTGTTACAGATGACATAGCTCCTGGTTA CCATAGCATTGTACAGAGGCCTATGGATTTGTCAACTATAAAGAAAAACATTGAAAATGGACTGATCCGA AGCACAGCTGAGTTTCAGCGTGACATCATGCTGATGTTTCAGAATGCTGTTATGTACAATAGCTCAGACC ATGATGTCTATCACATGGCAGTAGAGATGCAGAGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCAC ACAGTTGATTATGCAAACATCTGAGTCTGGAATCAGTGCTAAAAGTCTCCGGGGGAGAGACTCTACCCGA AAACAAGATGCTTCAGAGAAGGACAGTGTCCCCATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATG GGGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289607
Insert Size	2646 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289607.1, NP_001276536.1
RefSeq Size	4520 bp
RefSeq ORF	2646 bp
Locus ID	78656
UniProt ID	Q8R3B7
Gene Summary	May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter isoform (3), compared to isoform 1.

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