Rbbp8 (NM_001252495) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9930104E21Rik; CtIP; RBBP-8; RIM; SAE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229119 representing NM_001252495 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATTTCAGGAAGCGGCTGTGGAAGCCCTAATTCTGCGGATGCATCCAATGACTTCAAGGAACTCT GGACAAAACTAAAAGAGTATCATGATAAGGAAGTGCAAGGTTTACAAGTCAAAGTAACCAAACTGAAAAA GGAACGAATTTTAGATGCACAGAGACTAGAAGAATTCTTCACCAAAAATCAACAGCTGAGAGACCAGCAG AAAGTCCTTCAGGAAACCATTAAAATTTTAGAAGATAGGTTAAGAGCAGGATTGTGTGATCGCTGTGCAG TAACTGAAGAGCATATGCACAAAAAGCAGCAGGAGTTTGAAAATATCCGACAGCAGAACCTTAAGCTTAT TACAGAGCTGATGAATGAAAAGAATACTCTTCAAGAAGAAAACAAAAAGCTTTCTGAACAACTGCAGCAG AAAATGGAAAATGGTCAACAGGATCAAGTAGCTGAGCTTGCATGTGAGGAAAACATTATTCCAGACTCAC CAGTAACATCCTTTTCCTTTTCTGGCATTAACCGGCTACGAAAGAAGGAGAATCTCCATGTCCGATACGT GGAACAAACACATACTAAGTTGGAGCGCTCTCTATGTACAAATGAATTAAGAAAAATATCTAAGGATTCA GCTCCTGCACCAGTTAACTCTGAGGAACATGAAATTCTCGTAGCTGACACTTGTGATCAAAATCACTCAC CACTGTCAAAAATATGTGAAACAAGCAGTTACCCCACTGATAAGACATCTTTTAATTTAGACACTGTTGT TGCTGAGACACTTGGACTTAATGGTCAAGAAGAATCTGAACCCCAAGGTCCCATGAGCCCTCTGGGAAGT GAACTCTACCACTGTCTCAAAGAAGATCACAAAAAACACCCTTTTATGGAATCTGCAAGAAGTAAGGAAG ATAGCTTAAGATTTTCAGACTCTGCTTCAAAGACACCTCCTCAAGAATTTACTACTCGGGCGTCATCTCC GGTATTTGGAGCTACCTCTACTGTGAAAGCTCATTTGGGTTTGAATACAAGTTTCTCCCCCTCTCTCTTA GACATTGGGAAAAAAAACCTTCTGAAGACAGCTCCCTTTAGCAACATTGCTGTTTCTAGATCAGAGAAAG TTAGATCCAAATCTGAGGATAATGCCCTTTTCACACAACATAGTCTTGGGTCAGAAGTGAAGGTCATTAG CCAGTCATTTTCTAGTAAACAGATACTTACAAATAAAACTGTAAGTGACTCTGTAGATGAACAGTGCAGT GCTGACCACATGAATACTACTGTTGCTGATAAATATTTGGTGCCTTTGAAATCCCTGGGAGGCAAAGCAT CCAAAAGGAAGAGGACTGAGGAGGAAAGTGAGCATGCAGTAAAGTGCCCCCAGGCCTGTTTTGATAAGGA AAATGCCCTTCCTTTCCCAATGGAGAATCAGTTCTCCATGAATGGAGACCATGTGATGGATAAACCTCTG GACCTGTCAGATCGGTTTGCAGCTACTCAGCGGCAAGAGAAGAACCACGGAAACGAGACTTCTAAAAACA AACTTAAGCAAGCCACTATTTACGAGGCTTTGAAGCCCATTCCAAAGGGCTCCTCCTCAGGCCGTAAAGC CTTGAGTGGGGACTGCATGCCAGCCAAAGACTCTTGGGAGACATACTGCTTACAGCCGAGGAGCCTCCAG TCCTCTAGTAAATTCTCTCCAGACCAAAAAACACCATTACAGATAAAAGAAGAAAATCCTGTCTTCAAAA CACCTCCATGTTCACAGGAAAGTTTGGAGACAGAGAATCTCTTTGGTGATGTGAAGGGTACTGGTTCTCT TGTGCCAACAAAAGTAAAGAGCAGAGCAGTCCATGGAGGATGTGAGCTTGCATCAGTTCTTCAGTTAAAC CCCTGTAGAGTTGCTAAAACAAAGGCTCTGCCAAGCAACCAAGATACGTCCTTTGAAAATATCCAGTGGA GTGTAGACCCAGGAGCAGACCTTTCTCAGTATAAAATGGATGTTACTGTAATAGATACAAAGGATAGCAG TCACTCACGATTAGGAGGAGAAACAGTGGACATGGACTGTACATTGGTCAGTGAAACCGTGCTTTTAAAA ATGAAGAAACAAGAGCAAAAAGAAAGAAGTCCAAATGGAGACATAAAAATGAATGATAGCCTAGAAGATA TGTTTGATCGGACAACACATGAAGAGTATGAGTCCTGTTTGGCAGATAGCTTCTCCCAGGTACCAGATGA GGAAGAACTGCCTGACACCACAAAGAAAACAAACATACCTGCTGATAAACAAGATGGAGTCAAACAGAAA GCATTTGTGGGGCCATATTTTAAAGATAAGGAGAGAGAAACTAGCATACAGAATTTTCCTCATATTGAGG TAGTTCGGAAAAAAGAAGAGAGAAGGAAATTGCTTGGGCACACATGTAAAGAATGTGAAATTTATTATGC AGATTTGCCAGCAGAAGAAAGAGAAAAGAAGTTGGCTTCCTGCTCAAGACACCGATTTCGCTACATTCCA CCCAACACACCAGAGAATTTCTGGGAAGTTGGTTTTCCTTCTACTCAGACGTGTCTGGAAAGAGGTTATA TCAAGGAAGATCTCGATCTTAGTCCTCGTCCCAAAAGACGGCAGCCTTACAACGCGGTCTTTTCTCCAAA AGGCAAAGAACAGAGGACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252495
Insert Size	2682 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252495.1, NP_001239424.1
RefSeq Size	3523 bp
RefSeq ORF	2682 bp
Locus ID	225182
UniProt ID	Q80YR6
Gene Summary	Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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