Sulf2 (NM_001252579) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010004N24Rik; AU020235; mKIAA1247; MSulf-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229157 representing NM_001252579 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAATCCTGAGGGAAGAGGGGAAGGAATCCCATCCTCACGACACCACCTCGGCCTCTGCATCCAGG AAGAAGCAAAGGACCAGCAAGCCACGCCAATGGCACCCCCTGGCCTGCCACTATGGCTGCTGTCCACCGC TCTCCTCTCCCTGCTGGCTGGCAGCTCGGCCTTCCTCTCCCATCCCCGCCTGAAGGGACGCTTCCAGAGG GACCGCAGGAACATCCGGCCCAACATCATCTTGGTGCTTACGGATGACCAGGATGTGGAGCTGGGCTCCA TGCAAGTGATGAACAAGACAAGGCGTATCATGGAGCAGGGCGGGGCGCACTTCATCAATGCCTTCGTGAC TACACCAATGTGCTGTCCGTCTCGCTCCTCCATTCTCACCGGCAAGTACGTCCACAACCACAACACCTAC ACCAACAATGAGAATTGTTCCTCGCCCTCCTGGCAGGCCCAGCACGAGAGCCGCACCTTCGCCGTGTATC TCAACAGCACAGGCTACCGGACAGCTTTCTTCGGAAAATACCTCAATGAGTACAACGGCTCATACGTGCC GCCCGGCTGGAAGGAGTGGGTCGGCCTACTTAAGAACTCCCGCTTTTATAACTACACACTCTGCCGGAAT GGGGTGAAGGAGAAACATGGCTCAGACTACTCCACGGATTACCTCACGGATCTCATCACCAATGACAGTG TGAGCTTCTTCCGAACATCCAAGAAGATGTACCCACACAGGCCCGTGCTCATGGTCATCAGCCACGCGGC TCCCCATGGCCCCGAGGACTCAGCACCCCAGTACTCACGGCTCTTCCCCAATGCGTCCCAGCACATCACA CCGAGTTACAACTATGCACCCAACCCAGACAAGCATTGGATCATGCGCTACACGGGACCCATGAAGCCCA TTCACATGGAATTCACCAACATGCTACAACGAAAACGCCTACAGACCCTCATGTCTGTGGATGACTCCAT GGAGACGATCTATGACATGCTGGTGGAGACGGGGGAGCTGGACAACACGTACATCCTGTACACCGCCGAC CACGGCTACCACATTGGCCAGTTTGGGCTGGTGAAGGGCAAGTCTATGCCGTATGAATTCGACATCAGAG TCCCGTTCTACGTGAGGGGCCCCAACGTGGAAGCTGGCTCTCTGAACCCCCACATTGTCCTCAACATTGA CCTGGCCCCCACCATACTGGATATCGCTGGACTGGACATCCCTGCAGACATGGACGGGAAGTCTATTCTC AAACTACTGGACTCAGAGCGGCCAGTGAACCGGTTCCACTTGAAAAAGAAGCTGAGGGTCTGGCGAGACT CCTTCCTGGTGGAGAGAGGCAAACTGCTCCACAAGAGGGAGGGTGACAAAGTGAATGCCCAGGAGGAGAA CTTCCTGCCCAAGTACCAGCGCGTGAAGGACCTGTGTCAGCGAGCTGAGTACCAGACAGCATGCGAACAG CTGGGGCAGAAGTGGCAGTGTGTGGAGGACGCTTCTGGGACGCTGAAGCTGCACAAATGTAAAGGCCCCA TGCGGTTTGGTGGCGGCGGTGGCAGCAGAGCCCTCTCCAACCTGGTGCCCAAGTATGACGGCCAGAGCAG CGAGGCCTGCAGCTGTGACAGTGGCGGTGGAGGGGACTACAAACTGGGCCTGGCTGGACGCCGTAAGCTC TTTAAGAAAAAGTATAAGACCAGCTATGCCCGGAACCGCTCCATCCGTTCCGTGGCCATCGAGGTGGACG GTGAGATATACCACGTAGGCTTGGATACTGTGCCTCAGCCCCGCAACCTTAGCAAGCCGCACTGGCCAGG GGCCCCTGAAGACCAAGATGACAAGGATGGTGGCAGTTTCAGTGGTACTGGTGGCCTTCCAGATTATTCT GCCCCCAATCCCATCAAAGTGACCCATCGGTGCTACATCCTTGAGAATGACACAGTCCAGTGCGACTTGG ACCTGTACAAGTCCCTGCAGGCTTGGAAAGACCACAAGCTGCACATCGACCATGAGATCGAAACCCTGCA GAACAAAATTAAGAACCTTCGAGAAGTCAGGGGTCACCTGAAGAAGAAGCGACCGGAAGAATGTGACTGC CATAGAATCAGTTACCACAGCCAACACAAAGGCCGTCTCAAGCACAAAGGCTCCAGCCTGCACCCTTTCA GGAAGGGTCTGCAGGAGAAGGACAAGGTGTGGCTGCTGCGGGAGCAGAAACGCAAGAAGAAACTGCGCAA GCTGCTCAAACGGCTGCAGAACAACGATACGTGCAGCATGCCCGGCCTCACGTGCTTTACCCACGACAAC CACCACTGGCAGACGGCGCCACTCTGGACGCTGGGGCCGTTCTGCGCCTGCACCAGCGCCAACAACAACA CGTACTGGTGCTTGAGGACCATAAATGAGACCCACAACTTCCTCTTCTGCGAATTTGCAACCGGCTTCAT AGAATACTTTGACCTCAGTACAGACCCCTACCAGCTGATGAACGCGGTGAACACACTGGACAGGGACGTC CTTAACCAACTGCACGTGCAGCTCATGGAGCTAAGGAGCTGTAAAGGCTACAAGCAGTGCAACCCCCGGA CCCGCAACATGGACCTGGGGCTTAGAGACGGAGGAAGCTATGAACAATACAGGCAGTTTCAGCGTCGAAA ATGGCCAGAAATGAAGAGACCTTCTTCCAAATCACTGGGACAGCTATGGGAAGGTTGGGAAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252579
Insert Size	2727 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252579.1, NP_001239508.1
RefSeq Size	3794 bp
RefSeq ORF	2727 bp
Locus ID	72043
UniProt ID	Q8CFG0
Gene Summary	Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate upstream start codon compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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