Pcsk6 (NM_001291184) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	b2b2830Clo; C86343; Pace4; SPC4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229203 representing NM_001291184 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCGCGCGCGCCGCCAGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGATGTCGCGCGGGGCG CAGGGGCCGCCGGCCGGCACGGACTCCCGCCGCTCGCGCTGCGCCCCTGGCGTTGGCTGCTTCTGCTCGC GCTGCCCGCCGCCTGCTCCGCGCTGCCGCCGCCGCGCCCCGTCTACACCAACCACTGGGCAGTGCAAGTG CTGGGCGGCCCCGGCGCGGCGGACCGCGTGGCTGCGGCGCACGGCTACCTCAACTTGGGCCAGATTGGAA ACCTGGACGATTACTATCATTTTTACCACAGCAAGACCTTCAAGAGATCGACCTTGAGCAGCAGGGGCCC CCACACCTTCCTCAGAATGGACCCACAGGTAAAATGGCTGCAACAACAGGAAGTGAAGCGCAGAGTCAAG AGACAGGCGCGAAGTGACTCTCTTTATTTCAATGATCCCATTTGGTCTAACATGTGGTACATGCATTGTA CTGATAAGAACAGTCGCTGTCGGTCAGAGATGAACGTCCAGGCGGCGTGGAAGCGTGGCTACACAGGAAA GAATGTGGTCGTCACCATCCTCGATGACGGCATAGAAAGGAATCACCCAGACCTGGCCCCCAACTACGAT TCCTATGCAAGCTACGATGTCAATGGAAATGATTATGACCCATCCCCGAGATATGACGCCAGCAACGAGA ACAAACATGGCACTCGCTGTGCGGGTGAAGTCGCTGCCTCGGCCAACAACTCCTACTGCATCGTGGGCAT CGCATATAATGCAAAGATAGGAGGCATCCGGATGCTGGACGGCGATGTGACCGATGTGGTGGAGGCCAAG TCTCTGGGCATCAGACCCAACTACATTGACATTTACAGCGCCAGTTGGGGACCCGATGATGACGGAAAGA CAGTAGATGGACCAGGCCGGCTGGCTAAACAAGCTTTCGAGTATGGCATTAAAAAGGGTCGCCAAGGTCT GGGCTCCATTTTTGTTTGGGCCTCTGGGAATGGTGGGAGAGAAGGGGACCACTGCTCCTGTGATGGCTAC ACCAACAGCATCTACACCATCTCCGTAAGCAGCACCACTGAGAACGGCCACAAACCCTGGTACCTGGAAG AATGCGCTTCCACGTTGGCTACCACCTACAGCAGCGGGGCCTTCTATGAACGGAAGATCGTCACCACAGA TCTGCGTCAGCGCTGCACCGACGGCCACACTGGGACATCAGTCTCAGCCCCCATGGTGGCTGGTATCATT GCCTTGGCTCTAGAAGCAAACAACCAGTTGACCTGGAGGGATGTGCAGCACCTGCTGGTGAAGACATCAC GGCCAGCTCATCTGAAGGCGAGCGACTGGAAAGTCAACGGAGCTGGGCATAAAGTTAGCCATCTCTATGG ATTTGGCTTGGTGGATGCAGAAGCTCTGGTCCTGGAGGCGAGGAAGTGGACGGCAGTGCCCTCCCAGCAC GTATGCGTGGCCACCGCAGACAAAAGGCCCAGGAGCATCCCCATAGTGCAGGTGCTACGGACCACAGCCC TGACCAACGCCTGTGCGGACCACTCAGACCAGCGTGTGGTGTACCTGGAGCATGTGGTTGTCCGAATCTC TATCTCACACCCACGCCGGGGCGATCTCCAGATCCACCTGATTTCTCCCTCTGGAACCAAGTCTCAACTT TTGGCAAAGAGATTGCTGGATTTTTCCAATGAGGGGTTCACAAACTGGGAATTCATGACCGTCCACTGCT GGGGAGAAAAGGCTGAAGGGGAGTGGACTCTGGAAGTGCAGGATATACCATCGCAGGTCCGAAACCCAGA GAAACAAGGAAAGCTGAAAGAATGGAGCCTCATTTTATATGGCACCGCAGAGCACCCATATCGCACCTTC AGCTCCCATCAGTCTCGCTCACGGATGCTGGAGCTCTCCGTCCCGGAGCAGGAGCCTCCCAAGGCTGCTG GACAACCACCCCAGGCAGAGACTCCAGAAGACGAGGAAGAGTACACAGGTGTGTGCCATCCCGAGTGTGG TGACAAAGGCTGTGATGGTCCCAATGCAGACCAGTGCTTGAACTGTGTCCACTTCAGCCTGGGGAACTCC AAGACAAACAGGAAGTGTGTGAGCGAGTGCCCCTTGGGCTACTTTGGGGATGCAGCTGCAAGACGCTGCC GTCGCTGCCATAAGGGATGTGAGACGTGCACGGGCAGGAGCCCAGCACAGTGCCTGTCTTGTCGCCGTGG GTTCTATCACCACCAGGAGACGAACACGTGTGTGACCCTCTGTCCTGCCGGACTTTATGCTGATGAAAGT CAGAGACTCTGCCTCAGGTGCCACCCGAGCTGTCAGAAGTGCGTGGATGAACCTGAGAAGTGCACTGTGT GTAAGGAGGGATTCAGCCTGGCACGGGGCAGCTGCATTCCGGACTGTGAACCGGGTACCTACTTCGATTC AGAGCTCGTCAAATGTGGGGAATGCCATCACACCTGCCGGACCTGTGTGGGGCCCAGCAGAGAAGAGTGT ATTCACTGTGCAAAAAGCTTCCACTTCCAAGACTGGAAATGTGTGCCGGCCTGCGGTGAGGGCTTCTACC CGGAGGAGATGCCTGGCTTACCCCACAAAGTGTGTCGAAGATGTGAGGAGAACTGCCTGAGCTGCGAGGG CTCCAGTAGGAACTGCAGCAGATGTAAAGCCGGCTTCACACAGCTGGGAACCTCCTGCATCACCAACCAC ACGTGCAGCAATGCCGATGAGACCTTCTGCGAGATGGTGAAGTCCAATCGGCTCTGCGAACGGAAGCTCT TCATCCAGTTTTGCTGCCGCACCTGCCTCCTGGCTGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291184
Insert Size	2841 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291184.1, NP_001278113.1
RefSeq Size	4259 bp
RefSeq ORF	2841 bp
Locus ID	18553

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