Lig3 (NM_001291246) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D11Wsu78e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229269 representing NM_001291246 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTGGCTTTCAAGATCCTCTTCCCGAGAAACCTTTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT TCCCAGAACAGAATCGCTGGGCTGCCATAAGTCAGTTCAGCCAGTGGTCGGAAACAAATCTCCTCGGTGG GTGCTGCCTTCTCCAGAGAAGAAAGCCTGTCCTAGCTCTCCAGAGAGGTCATCTAAGACCACGTGCCACC CACCTTACTTTCTGGCCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGCGATGGCAGAGCAGCGGT TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGATGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT GTGCCGCATTGGCAAAGTGGTGCCCAACCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC ATTAAGTGCATGTTTGAGAAACTGGAACGGGCTCGGGCCACCACAAAGAAAATTGAAGACCTCACAGAGC TGGAAGGCTGGGAAGAGCTGGAAGATAACGAAAAGGAACAGATAAGCCAGCATATTGCAGACCTGTCTTC TAAGGCAGCTGCCACACCTAAGAAGAAAGCTGCTGTCCAGGCTAAGCTGACAACCACTGGCCAGGTGACA TCTCCAGTGAAAGGTGCTTCGTTTATTACCAGTACCAATCCTCGGAAGTTCTCTGGCTTTTCAGCAAAAC CCAACAACTCTGAGCAAGCCCCCTCAAGCCCTGCTCCTGGAACGAGTCTGTCTGCAAGTAAATGTGACCC TAAGCACAAAGATTGTCTGCTACGAGAGTTCCGGAAGCTGTGCGCCATGGTGGCTGAAAATCCTAGCTAT AACACCAAGACCCAGATCATCCATGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTTCACGGTGATG TGTACCTGACAGTTAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTTTACAACTTGAATGATAAGCAGAT TGTAAAACTTTTTAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGTGAC GTGTCAGAGACGATCAGAATCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAAAGCCTCCTCA CCATTCAGGAAGTGGATGCCTTCCTCCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCAGGC CCTGCAGGACATAGCCTCCCGGTGTACAGCCAATGACCTTAAGTGCATTATTCGGCTGATCAAACATGAT CTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCAAAG CATCGCGCAACCTGCAGGATGTGGTGGAGCGAGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCCAGG CCGGCGACGGGCTCTGAGGGTCCAGGCCTCACTGATGACGCCTGTGCAGCCCATGCTGGCTGAGGCCTGC AAATCCATTGAATATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTGAGC GAGTCCAGGTGCATAAGAAGGGGGACCACTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCCTGCCTCA CAAGGTGGCCCACTTCAAGGACTATATACCCAAAGCTTTTCCTGGGGGTCAAAGCATGATCTTGGATTCC GAGGTACTCCTGATTGACAACAACACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTGCACAAGAAAG CTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGAT GGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGACAACATGGTTGAAATCCGTAACCGGATCATG TTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAGAGG GCTTGGAAGGGTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAAAGT TAAGAAAGATTATTTGAATGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTCTAT GGGCAAGGGAGCAAAGGCGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCTGACAGCCAAAAGT GGTGCACTGTTACAAAATGTGCTGGAGGCCATGATGATGCCACACTTGCCCGCCTGCAGAAAGAGCTGGT TATGGTGAAGATCAGCAAGGATCCTAGTAAGATACCCAGCTGGCTGAAGATCAACAAGATCTATTATCCT GACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGGGCAGAATTCTCCAGATCTG AGGCACACACTGCTGATGGCATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGACGACAAGGACTG GAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTTGCT GTGGTGGCTGGAGATGAGGCAAGCCCCACTACTGGAGGCAGCAGTGGCGAGAACGAGGGCACTGCTGGGT CTGCTGGGCCCTGCAAGGGCCCCCCCAGTAAGTCCTCTGCCAGTGCCAAGACCACCGAACAGAAGCTCAA TAGCCCCAGTAGCAGAGGTGGCATCAAACCGATTCCAAAGCATTCCCCCATGAAACCAGGAGAGAAGCTG GCTGTGAAGTCTTCTCCAGTCAAAGTGGGAATGAAGAGGAAAGCTACTGATGAGACCCCATGCCTGAAAA AGGTACTGTTGGATGTCTTCACTGGGGTGCGGCTCTACTTGCCACCTTCTACACCAGACTTCAAACGTCT CAAACGCTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGACATGGGCTCAGCCACACATGTG CTAGGTAACAGGGAAAAGAACACTGATGCCCAGTTGGTCTCCTCAGAGTGGATTTGGGCATGTATCCGGA AACGGAGGCTGATAGCTCCCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291246
Insert Size	3036 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291246.1, NP_001278175.1
RefSeq Size	5981 bp
RefSeq ORF	3036 bp
Locus ID	16882

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