Ano1 (NM_001242349) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Tmem16a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229275 representing NM_001242349 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGACGCGCAGGACAGCGACATCGGCCTGGAGGGCTTGACCCCAGAGGGAACCTCTGCGGACCGAG AGTGCCAGCGGGGACCGGAAACAATCGCGCACGAGGCACAGGACGCAGGAACCCCCAACTCAGGTGACGC CACCGGGGTTGTGGATGGGGAGCGCGAGGCCACCATGAGGGTCCCCGAGAAGTACTCGACGCTCCCGGCG GAGGACCGCAGCGTCCACATCGTGAACATCTGCGCCATCGAGGACCTGGGCTACCTGCCGTCCGAGGGCA CGTTGCTGAACTCTCTGTCCGTGGACCCCGACGCCGAATGCAAGTATGGACTATACTTCAGGGATGGCAA ACGGAAGGTGGACTACATCTTGGTATACCATCACAAGAGAGCCTCAGGGAGCAGGACTCTGGCCAGGAGG GGACTACAAAATGACATGGTCCTGGGGACCCGCAGCGTCAGGCAGGACCAGCCCCTTCCCGGGAAGGGGA GCCCTGTGGATGCAGGCTCACCGGAAGTCCCCATGGATTACCATGAAGATGACAAACGCTTCAGACGGGA GGAATATGAGGGCAACCTGTTGGAGGCAGGCCTGGAGTTGGAGAATGACGAGGATACCAAAATCCATGGT GTCGGGTTTGTGAAGATCCATGCGCCCTGGCATGTGCTCTGTAGGGAAGCTGAGTTTTTGAAACTAAAGA TGCCCACAAAGAAGGTGTACCACATCAGTGAGACGCGAGGCCTCCTGAAAACCATCAACTCGGTTCTGCA GAAGATCACAGACCCCATCCAGCCCAAGGTGGCTGAGCACAGGCCACAGACCACAAAGAGGCTCTCCTAT CCCTTCTCCCGGGAGAAGCAACACCTATTCGACCTGACTGACAGGGACTCTTTTTTCGACAGCAAAACCC GGAGCACAATAGTCTATGAAATCCTGAAGAGAACAACGTGCACCAAGGCCAAGTACAGCATGGGTATCAC CAGCCTCCTGGCCAATGGCGTATACTCAGCTGCATACCCTCTGCACGATGGGGACTATGAGGGTGACAAC GTTGAGTTCAACGACAGGAAACTCCTGTATGAGGAATGGGCAAGTTACGGAGTCTTCTACAAATACCAGC CCATTGACCTGGTCAGGAAATACTTTGGTGAGAAGGTTGGCCTGTACTTTGCCTGGCTTGGAGCCTACAC CCAGATGCTCATCCCTGCCTCGATCGTGGGTGTCATTGTCTTTCTCTATGGATGTGCCACTGTGGACGAA AACATCCCCAGTATGGAGATGTGTGACCAGAGATACAACATCACCATGTGTCCTCTGTGTGACAAGACCT GCAGCTACTGGAAGATGAGCTCAGCCTGTGCCACAGCCCGTGCCAGTCACCTTTTTGATAACCCTGCCAC CGTCTTCTTCTCTGTGTTTATGGCCCTCTGGGCTGCCACTTTCATGGAGCACTGGAAACGGAAGCAGATG AGGCTCAACTACCGATGGGACCTCACAGGCTTCGAGGAGGAGGAGGATCATCCCAGAGCAGAGTATGAAG CCAGAGTCTTAGAGAAGTCACTGAGAAAAGAATCCAGAAACAAAGAGACCGACAAGGTGAAGCTGACCTG GAGGGACCGATTCCCAGCCTATTTCACCAATCTTGTCTCCATCATCTTCATGATCGCAGTGACATTTGCA ATCGTCCTCGGAGTTATCATCTATAGAATCTCCACAGCTGCAGCCTTGGCCATGAACTCCTCCCCGTCTG TGCGGTCCAACATCCGGGTTACAGTCACGGCCACCGCTGTTATCATCAACCTCGTGGTCATCATTCTGCT GGATGAAGTTTACGGCTGCATTGCCAGGTGGCTCACCAAGATTGAGGTCCCAAAGACAGAGAAGAGCTTT GAGGAGAGGCTAACCTTCAAGGCCTTCCTGCTCAAGTTTGTGAACTCTTACACTCCCATCTTCTATGTCG CCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCCGCTCTTTCCGGATGGA GGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCAAGCAG CTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAAGCTGC GCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTCAACCT CGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCCTGTTT GTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGATGCCA AAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTATAACAT CCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACTTCATC CCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACACGCTCT CTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTATGAGGT TCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCAAAGAC TTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCATGAGTG ACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAGGTTCT CATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGAAGGAG AAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAGCCCAG TCCCCAGCTACGAGTACCATGGGGACGCGCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242349
Insert Size	3045 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242349.1, NP_001229278.1
RefSeq Size	4605 bp
RefSeq ORF	3045 bp
Locus ID	101772
UniProt ID	Q8BHY3
Gene Summary	Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and lacks an internal coding exon, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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