Uspl1 (NM_001286824) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E430026A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229342 representing NM_001286824 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGATGATTCTCTGAAGATTGGAAATGGTTTGCCACTGGTTGGACCAGGGACTGATAATTATGCTT CAGCTAAAGTCACAACAGACGGGCATTGCCCTGCTTGTAGAGCGAAAGGCAAGCTAAGTGCCTTAAAACC TTACCGCATTAGCTTCCAAGAATCTGTCTTTCTGTGTGAGGATCTTCAGTGCATTTATCCTTTGGGCTCT GAGTCACTTACTAATTTAATTTCTCCTGATTCAGAAGATTGTCCCACTCCAAGTAAGCCTCAAAAACGAA AAAGATTGGAAACCAACTGTAGGAATTCACCTCTTCCAGTACATTCTAAAAAGACTAGAAGTCACATTGT CACTGATAGTGAACCAATTGTGAATGGTAAATATAATGGAGAAGTGTGTGATGACTTCTCAGCAAGCTTC CCAGATACCAGTGCTCACCAGGATCCTGCCAGCACAGCTGCTTCTGTGGAGCAGAGTGAGGCTTTGGAGG CTGATGATGTGGTTGTGGCCGCTACAGAAGATCCTGCTACAGTTAGTGTCACATCTGAGCTGGAAATGCC AGCTAAGAGCAGATGTTTACCACTTTGCCAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCCTGTGC TGGTTAGACTGTATCCTGTCAGCCCTGGTGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGCGTGCT CCAGGGAGGAGTGTGTATTTGGAAGGCTGTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCACACCCA TCACCTGCATGGCGTCACAGGTGAAGATTGTAAAAAATTGACATCAGAAATATTTACAGAAATAGATACC TGTTTGAATAAAGTTAGAGATGAAATTTTTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGGTGACA TGGAAAGTCCTGTGTTTGCACTTCCTGTACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTTACATA TTCTTTTTCTTGGAATTTTGAATGTTCCCATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGAGTCTG GTCACCTTTACCAATATTGTTCCTGAGTGGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAACAGCT GCAACAGTAAATCACAAATAAGAAAAATGGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCACTTCGT GGAGGGCTTACCAAGGAGGGATTTGCAACACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAGTCACG TCGGTAATACAGTACCAAGCAAATAACCATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTGGCTGG AGTGTGATGACCTAAAGGGTCCCTGTGCTAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAGACACA TATCGTTATTTGGGAAAGAAAATCCCAGGTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGAAGCCA AATGTGCAGCCTGTATCAGGTGAGGAGCAGCCGACTTGTCCAGCACTGTGTTCTCTGGCTGGGACCGCCA CCTCAGAACCCTCAGTCGCCCATCCCACATCTATGGCAGGCGCTCCTCAGACTCTTCCAGAAATCCAAGC TGTAGCTCATGGAGATAGTGTACTTTCAGGAGCAAAAGGCATGGTCGACAGCATTTTACCCTCAGCACTT GAAGAAACCATCCAGGAAACTGCCTCTGTTTCTCAGGTTGACTCCAAAGATTGCCTGTTGGAAGACAAAC CCGTGGCAGGAAGTGCAGCACTGGTCAGAGTACTTGCTTTTCAGCCACAGGACTCCCCGGGGTCTTCGGG GTCTTCCTTAGTGTCCAGCCTGTGTGAAGGAAAGCTAGTTGCCCCATGTGTGGATTCAAGTTTCCCATCC CAAGCTGTGAGCACAGACCTGCAGGCAGTACTGTCCCAGGCAGGGGACACTGTGGTTCCTAACCCTGTGA CTGATGCCCCTGTTCCTGTTCTTGTTCAGGAATTGAAGTCCCTGGCAACTGAGAAGGACTCTCAGACACA GTTGCTGCCACTGAAAACTGAGAAGTTAGATCCTGAGCAACCTGGTAAATCTCAGGCATCTAATTTGAGA AAACGAGAAACCACAGCGTCTTCTAAAACAGTAGCAGCCAGGTCAGCACAGAATCAGCCTCGGAAAGAAG ATCAGAAGAGAGCCTTCGTGGGAAGTTGGGTGAAGGGGCTATTGAGCAGGGGTGGTGCTTTTATGCCAAC CTGTGTTTTGTCTCAGAGTAGAGCAGTAAGTGACCTGCAACCTTCTGTTAAAGGAGCCAGTAATTTTGAT GGCTTTAAAACGAAAAGTATAAGCCGCAGGTCTAAAAGAATGTCCAGGAAAGCTAAGCACATGGAGGAAC TGTCTCCAAGGAACAGCTCTCCACCATTGAGCTGGACAGCTGCTCTGACCCAGGCTGCTGAGAACGCCAC CTCAGCTCTGTTGAGGGAGCAGGAAGGCTCACGTCCAGCTCCCCTCAGGCACAGATCTCCTGGCAATGAA AGCGCCATCTCTCCAGCAAGCCGTGGAGATGCGGCTGAAGATCAGGTTCATAAGCTTCGGCTCAAACTTC TTAAAAAATTAAAGGCAAAAAAGAAGAAACTAGCTGCCCTCATTTCTTCCCCGCACCGTGAACCATCTCT AAGTGACCACTCAGAGCCTGCGTCCCATTGTGGAACACCTGCAAGTGACCAGTCAGAGCCTGTATCCCAT TGTGGATCTCCAAATGACTGTGAGTCCATAGAAGATCTGTTAAAAGAGCTACAGCATCAAATTGACCTTG CAGACAGCAAATCTGGGTGCACCTCTGCTCCTGATGCCACCTCAAATAACAGCCAGAGTCATGAGGAGAT TTTAGCAGAGTTGCTGTCGCCTACAGCCATGTCAGAGCCCTCAGAAAGTGGGGAGCTTGAGTTGAGGTAC TTGGAAATGGGGGACAGCACCCCAGCACAAGCACCTAGTGAATTCAGTGTTGTCTCCCAGAACACATGTC TGAAACAGGACCATGATTACTGCAGCCCTGAGAAAGGCCAGCGGGAAGTTGATCTGCATTCAGTCATGGA CAGTGCCTGTATAAGAACCTTGAACTTGGGGAGTCCCATGAAGACTGATATCTTTGATGATTTTTTTTCC ACCTCAGCACTGAATTCTTTAACAAACGACACATTAGACATACCTCATTTTGATGACTCTCTGTTTGAGA ATTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286824
Insert Size	3228 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286824.1, NP_001273753.1
RefSeq Size	3730 bp
RefSeq ORF	3228 bp
Locus ID	231915
UniProt ID	Q3ULM6
Gene Summary	SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform E, which is shorter than isoform A.

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