Daam1 (NM_172464) Mouse Untagged Clone

CAT#: MC229344

Daam1 (untagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 2



  "NM_172464" in other vectors (1)

CNY 10,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700066F09Rik; 2310028E21Rik; AI503486; E130308H01; mKIAA0666
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229344 representing NM_172464
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC
CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT
GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC
AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG
AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA
ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA
CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT
TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC
ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG
AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC
CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT
CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC
ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC
TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC
ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT
GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA
GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA
CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG
GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG
AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA
GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG
AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC
AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG
AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA
GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG
GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC
CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA
ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA
GACAGCAGGAGTTCTTTGTGAACAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTC
CAAACTTAAAGTCAAAGAGCTGTCAGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCG
AGGTTGAAATTATCCAATGATGAAATCAAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCA
AGGACATGTTAGAACAGCTTTTGAAATTCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAA
GCATGAGCTGGATCGGATGGCCAAGGCTGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAG
CAAAGACTGCAATCACTGTACTTCAAAAAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAG
AAGCGATTCGCTCTGGCTCTGAGGAGGTGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTT
GGCTTTTGGAAATTATATGAATAAAGGACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAAC
AAGATAGCGGATACCAAATCCAGTATTGACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGG
AGAATAAGTACCCCAAAGTCCTCAACCTAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAA
CATGACTGAGCTGGACAAGGAGATCAGCACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAG
TATCAGAAGTCTCAGCCCCCACAGCCTGGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGG
CTAGCTTCAGCTTCTCAGATGTTGAAGATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAA
GCACTTTGGGGAAGAGGCTGGCAAGATCCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAG
GCCGTGGCCGAAGCGAAACAGGAAAATGAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTC
GCCTGGAAGCTCAGCTCAAAGAGCAGCGGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGA
AGAGAGTGGAGAGTTTGACGACCTGGTCTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCA
AAACTCAAACGGAACCGGAAACGCATTTCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAA
AACTTAATTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_172464
Insert Size 3234 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172464.3, NP_766052.2
RefSeq Size 5888 bp
RefSeq ORF 3234 bp
Locus ID 208846
UniProt ID Q8BPM0
Gene Summary Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...