Itga6 (NM_001277970) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5033401O05Rik; AI115430; Cd49f; VLA-6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229362 representing NM_001277970 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCGCGGGCCAGTTGTGCCTGCTCTACCTGTCCGCGGGGCTTCTAGCCCGGCTGGGTACAGCCT TCAACCTGGACACCCGCGAGGACAACGTGATCCGGAAATCGGGGGATCCCGGGAGCCTCTTCGGCTTCTC GCTCGCCATGCACTGGCAGTTGCAGCCGGAGGACAAGCGGCTGTTGCTTGTGGGGGCACCTCGGGCAGAA GCACTCCCGCTGCAGAGGGCGAACAGAACAGGGGGCCTGTACAGCTGTGACATCACCTCCCGGGGACCTT GTACACGGATTGAATTTGATAATGACGCTGATCCTATGTCAGAAAGCAAGGAAGACCAGTGGATGGGAGT CACTGTTCAGAGCCAAGGTCCAGGGGGCAAAGTGGTGACGTGTGCACATCGATATGAGAAACGGCAGCAC GTCAACACGAAGCAGGAGTCGCGGGATATCTTTGGAAGATGTTATGTCCTAAGTCAGAATCTCAGAATTG AAGATGATATGGACGGAGGAGACTGGAGTTTCTGCGATGGCCGGTTGAGAGGCCATGAAAAGTTTGGCTC CTGTCAGCAAGGAGTAGCGGCTACTTTCACTAAGGACTTTCATTACATTGTTTTTGGAGCCCCAGGGACT TACAACTGGAAAGGGATCGTCCGTGTAGAACAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAG ATGGGCCCTATGAAGTTGGTGGAGAGACTGATCATGATGAAAGTCTCGTGCCCGTTCCTGCTAACAGTTA CCTAGGCTTTTCACTGGACTCAGGGAAGGGTATTGTTTCTAAAGATGACATCACTTTTGTGTCTGGTGCT CCAAGAGCCAATCACAGTGGGGCTGTAGTTTTGCTAAAAAGAGACATGAAGTCCGCGCATCTGCTCCCTG AGTATATATTTGACGGAGAAGGCCTGGCTTCCTCGTTTGGCTATGATGTGGCAGTGGTGGACCTCAATGC AGATGGGTGGCAAGACATTGTTATCGGAGCTCCACAGTATTTTGATAGGGATGGTGAAGTCGGGGGTGCA GTTTACGTCTACATTAACCAGCAAGGCAAATGGAGTAATGTGAAGCCGATTCGTCTAAATGGGACCAAAG ACTCGATGTTTGGAATCTCTGTGAAAAATATAGGTGATATTAACCAAGATGGCTATCCAGATATTGCTGT TGGAGCTCCCTATGATGATCTGGGGAAGGTTTTTATCTATCATGGATCCCCGACTGGCATAATTACCAAG CCAACACAGGTTCTCGAGGGGACATCGCCTTACTTCGGCTATTCAATCGCTGGGAATATGGACCTGGATC GGAATTCCTACCCCGACCTTGCTGTGGGCTCCCTCTCAGACTCGGTCACTATTTTCAGATCCCGGCCAGT GATTAACATTCTAAAAACCATCACAGTGACTCCTAACAGAATTGACCTCCGCCAGAAGTCCATGTGTGGC TCACCTAGCGGGATATGCCTCAAGGTTAAAGCCTGTTTTGAATATACTGCGAAACCTTCCGGTTATAACC CTCCAATATCAATTTTGGGTATTCTCGAAGCTGAAAAAGAAAGAAGAAAGTCAGGGTTGTCATCGAGAGT TCAGTTTCGAAACCAAGGTTCCGAGCCAAAGTATACTCAGGAGCTGACCCTGAATCGGCAGAAGCAGCGG GCGTGCATGGAGGAGACCCTCTGGCTGCAGGAGAACATCAGAGACAAGCTGCGTCCCATCCCCATCACGG CTTCTGTGGAGATCCAGGAGCCCAGCTCTCGCCGGCGGGTGAACTCACTCCCCGAAGTTCTTCCCATCCT GAATTCAAATGAAGCCAAAACGGTCCAGACAGATGTCCACTTCTTAAAGGAAGGATGTGGAGACGACAAT GTCTGTAACAGCAACCTTAAGCTAGAGTATAAATTTGGTACCCGAGAAGGAAATCAAGACAAATTCTCTT ACCTTCCAATTCAAAAAGGCATCCCAGAATTAGTCCTAAAAGATCAGAAAGATATAGCTCTGGAAATAAC GGTGACCAACAGCCCTTCGGATCCAAGGAATCCCCGGAAAGATGGCGACGATGCCCATGAAGCCAAACTC ATCGCCACGTTTCCAGACACTCTGACATATTCCGCTTACAGAGAACTGAGGGCTTTCCCTGAGAAGCAGC TGAGCTGTGTGGCCAACCAGAATGGCTCCCAAGCCGACTGTGAGCTCGGAAATCCTTTCAAGAGAAATTC CAGTGTTACTTTCTATCTGATTTTAAGTACAACCGAGGTCACCTTTGACACCACAGATCTGGATATTAAT CTGAAGTTGGAAACAACAAGCAATCAGGATAATTTGGCTCCAATTACAGCGAAGGCAAAAGTGGTTATTG AATTGCTTTTATCGGTCTCCGGAGTCGCTAAGCCTTCGCAGGTGTATTTTGGAGGTACAGTTGTTGGTGA GCAAGCTATGAAATCTGAAGATGAAGTAGGAAGTTTAATAGAGTATGAATTTAGGGTGATTAACTTAGGC AAGCCTCTTAAAAACCTCGGCACAGCAACCTTGAATATACAGTGGCCCAAGGAGATTAGCAATGGCAAAT GGTTGCTTTATTTGATGAAAGTTGAATCCAAAGGTTTGGAGCAGATTGTTTGTGAGCCACACAATGAAAT AAACTACCTGAAGCTGAAGGAGTCTCACAACTCAAGAAAGAAACGGGAACTTCCTGAAAAACAGATAGAT GACAGCAGGAAATTTTCTTTATTTCCTGAAAGAAAATACCAGACTCTCAACTGCAGCGTCAACGTGAGGT GTGTGAACATCAGGTGCCCACTGCGAGGGCTGGACAGCAAGGCCTCTCTCGTTCTTCGTTCCAGGTTGTG GAACAGCACATTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATTCTCCTGAGGGCTTCCATAGAT GTCACCGCTGCTGCTCAGAATATCAAGCTCCCTCACGCGGGCACTCAGGTTCGAGTGACGGTGTTTCCCT CAAAGACTGTAGCTCAGTATTCAGGAGTAGCTTGGTGGATCATCCTCCTGGCTGTTCTTGCCGGGATTCT GATGCTGGCTCTATTAGTGTTTTTACTGTGGAAGTGTGGATTCTTTAAGCGCTCTAGGTACGATGACAGC ATTCCCCGATACCATGCGGTGCGGATCCGGAAAGAAGAGCGAGAGATCAAAGATGAGAAACACATGGATA ACCTCGAAAAAAAACAGTGGATCACCAAGTGGAATGAAAACGAAAGTTACTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277970
Insert Size	3276 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277970.1, NP_001264899.1
RefSeq Size	5888 bp
RefSeq ORF	3276 bp
Locus ID	16403
UniProt ID	Q61739
Gene Summary	This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) lacks an alternate penultimate exon, compared to variant 1, resulting in a novel 3' coding region and shorter 3' UTR. It encodes isoform 2 which has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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