Acly (NM_001199296) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A730098H14Rik; AW538652
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229371 representing NM_001199296 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGG CCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCA CCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGC CTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCA CTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGA GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG GATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGG ACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGG TCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT GGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGG GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGA TGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCT GGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATT ACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTAT GACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTG GCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCA CCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC CACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC CACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGA GCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGC AAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCC ACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGG ACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCA CAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATG AAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGG AGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACG GAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAG CCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCC CAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGAC CACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCAC GTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGG AATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGAC CTGTGCCACCATGTTCTCCTCCGAGGTCCAGTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAA ACTGCAGTAGCCAAGAACCAGGCCTTGAAGGAAGCAGGAGTGTTTGTGCCCCGAAGCTTCGATGAGCTTG GAGAAATCATTCAGTCTGTGTATGAAGATCTGGTGGCCAAAGGAGCCATTGTACCTGCCCAGGAAGTGCC ACCTCCAACAGTGCCCATGGACTACTCTTGGGCCAGAGAGCTGGGTTTGATCCGAAAACCTGCCTCATTC ATGACCAGCATCTGTGATGAGCGAGGGCAGGAGCTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCA AGGAGGAGATGGGCATCGGTGGTGTCCTCGGCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTG CCAGTTCATTGAGATGTGTCTGATGGTCACAGCTGATCACGGGCCAGCTGTCTCTGGAGCCCATAACACC ATCATCTGTGCTCGGGCTGGGAAGGACCTGGTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGAGACC GGTTTGGGGGTGCCTTGGATGCCGCAGCAAAGATGTTCAGTAAAGCCTTTGACAGCGGCATCATTCCCAT GGAGTTCGTCAACAAGATGAAGAAGGAGGGGAAGCTGATCATGGGCATCGGCCATCGAGTAAAATCGATA AACAACCCAGACATGCGAGTGCAGATCCTCAAGGACTTCGTCAAACAGCACTTCCCCGCCACCCCGCTGC TCGACTATGCCCTGGAAGTGGAGAAGATTACCACCTCCAAGAAGCCAAATCTTATCCTGAATGTGGACGG CTTCATCGGCGTTGCGTTTGTGGACATGCTCAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGATGAA TATGTTGACATTGGAGCCCTCAATGGCATCTTTGTGCTAGGAAGGAGTATGGGCTTCATTGGGCACTACC TTGACCAGAAGAGGCTGAAGCAAGGGCTGTATCGTCACCCCTGGGATGACATTTCCTATGTTCTTCCAGA ACACATGAGCATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001199296
Insert Size	3306 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199296.1, NP_001186225.1
RefSeq Size	4438 bp
RefSeq ORF	3306 bp
Locus ID	104112
Gene Summary	ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis (PubMed:25450640). In nervous tissue it may be involved in the biosynthesis of acetylcholine (By similarity) (PubMed:25450640).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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