Acly (NM_001199296) Mouse Untagged Clone

CAT#: MC229371

Acly (untagged) - Mouse ATP citrate lyase (Acly), transcript variant 1



  "NM_001199296" in other vectors (1)

CNY 7,672.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A730098H14Rik; AW538652
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229371 representing NM_001199296
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGG
CCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCA
CCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGC
CTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCA
CTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGA
GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG
GATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGG
ACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGG
TCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT
GGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGG
GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGA
TGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCT
GGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATT
ACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTAT
GACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTG
GCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCA
CCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC
CACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC
CACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGA
GCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGC
AAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCC
ACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGG
ACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCA
CAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATG
AAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGG
AGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACG
GAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAG
CCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCC
CAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGAC
CACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCAC
GTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGG
AATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGAC
CTGTGCCACCATGTTCTCCTCCGAGGTCCAGTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAA
ACTGCAGTAGCCAAGAACCAGGCCTTGAAGGAAGCAGGAGTGTTTGTGCCCCGAAGCTTCGATGAGCTTG
GAGAAATCATTCAGTCTGTGTATGAAGATCTGGTGGCCAAAGGAGCCATTGTACCTGCCCAGGAAGTGCC
ACCTCCAACAGTGCCCATGGACTACTCTTGGGCCAGAGAGCTGGGTTTGATCCGAAAACCTGCCTCATTC
ATGACCAGCATCTGTGATGAGCGAGGGCAGGAGCTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCA
AGGAGGAGATGGGCATCGGTGGTGTCCTCGGCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTG
CCAGTTCATTGAGATGTGTCTGATGGTCACAGCTGATCACGGGCCAGCTGTCTCTGGAGCCCATAACACC
ATCATCTGTGCTCGGGCTGGGAAGGACCTGGTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGAGACC
GGTTTGGGGGTGCCTTGGATGCCGCAGCAAAGATGTTCAGTAAAGCCTTTGACAGCGGCATCATTCCCAT
GGAGTTCGTCAACAAGATGAAGAAGGAGGGGAAGCTGATCATGGGCATCGGCCATCGAGTAAAATCGATA
AACAACCCAGACATGCGAGTGCAGATCCTCAAGGACTTCGTCAAACAGCACTTCCCCGCCACCCCGCTGC
TCGACTATGCCCTGGAAGTGGAGAAGATTACCACCTCCAAGAAGCCAAATCTTATCCTGAATGTGGACGG
CTTCATCGGCGTTGCGTTTGTGGACATGCTCAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGATGAA
TATGTTGACATTGGAGCCCTCAATGGCATCTTTGTGCTAGGAAGGAGTATGGGCTTCATTGGGCACTACC
TTGACCAGAAGAGGCTGAAGCAAGGGCTGTATCGTCACCCCTGGGATGACATTTCCTATGTTCTTCCAGA
ACACATGAGCATGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001199296
Insert Size 3306 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001199296.1, NP_001186225.1
RefSeq Size 4438 bp
RefSeq ORF 3306 bp
Locus ID 104112
Gene Summary ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis (PubMed:25450640). In nervous tissue it may be involved in the biosynthesis of acetylcholine (By similarity) (PubMed:25450640).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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