Map4 (NM_001205330) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA407148; MAP-4; Mtap-4; Mtap4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229392 representing NM_001205330 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCTCAGTCTTGTGGATGCGTTGACAGAACCACCTCCAGAAATTGAGGGAGAAATAAAGCGAG ACTTCATGGCTGCGCTGGAGGCAGAGCCCTATGATGACATCGTGGGAGAAACTGTGGAGAAAACTGAGTT TATTCCTCTCCTGGATGGTGATGAGAAAACCGGGAACTCAGAGTCCAAAAAGAAACCCTGCTTAGACACT AGCCAGGTTGAAGGTATCCCATCTTCTAAACCAACACTCCTAGCCAATGGTGATCATGGAATGGAGGGGA ATAACACTGCAGGGTCTCCAACTGACTTCCTTGAAGAGAGAGTGGACTATCCGGATTATCAGAGCAGCCA GAACTGGCCAGAAGATGCAAGCTTTTGTTTCCAGCCTCAGCAAGTGTTAGATACTGACCAGGCTGAGCCC TTTAACGAGCACCGTGATGATGGTTTGGCAGATCTGCTCTTTGTCTCCAGTGGACCCACGAACGCTTCTG CATTTACAGAGCGAGACAATCCTTCAGAAGACAGTTACGGTATGCTTCCCTGTGACTCATTTGCTTCCAC GGCTGTTGTATCTCAGGAGTGGTCTGTGGGAGCCCCAAACTCTCCATGTTCAGAGTCCTGTGTCTCCCCA GAGGTTACTATAGAAACCCTACAGCCAGCAACAGAGCTCTCCAAGGCAGCAGAAGTGGAATCAGTGAAAG AGCAGCTGCCAGCTAAAGCATTGGAAACGATGGCAGAGCAGACCACTGATGTGGTGCACTCTCCATCCAC AGACACAACACCAGGCCCAGACACAGAGGCAGCACTGGCTAAAGACATAGAAGAGATCACCAAGCCAGAT GTGATATTGGCAAATGTCACGCAGCCATCTACTGAATCGGATATGTTCCTGGCCCAGGACATGGAACTAC TCACAGGAACAGAGGCAGCCCACGCTAACAATATCATATTGCCTACAGAACCAGACGAATCTTCAACCAA GGATGTAGCACCACCTATGGAAGAAGAAATTGTCCCAGGCAATGATACGACATCCCCCAAAGAAACAGAG ACAACACTTCCAATAAAAATGGACTTGGCACCACCTGAGGATGTGTTACTTACCAAAGAAACAGAACTAG CCCCAGCCAAGGGCATGGTTTCACTCTCAGAAATAGAAGAGGCTCTGGCAAAGAATGATGAGTCCTCTGC AGAAATACCTGTGGCTCAGGAGACAGTGGTCTCAGAAACAGAGGTGGTCCTGGCAACAGAAGTGGTACTG CCCTCAGATCCCATAACAACATTGACAAAGGATGTGACACTCCCCTTAGAAGCAGAGAGACCGTTGGTGA CGGACATGACTCCATCTCTGGAAACAGAAATGACCCTAGGCAAAGAGACAGCTCCACCCACAGAAACAAA TTTGGGCATGGCCAAAGACATGTCTCCACTCCCAGAATCAGAAGTGACTCTGGGCAAGGACGTGGTTATA CTTCCAGAAACAAAGGTGGCTGAGTTTAACAATGTGACTCCACTTTCAGAAGAAGAGGTAACCTCAGTCA AGGACATGTCTCCGTCTGCAGAAACAGAGGCTCCCCTGGCTAAGAATGCTGATCTGCACTCAGGAACAGA GCTGATTGTGGACAACAGCATGGCTCCAGCCTCCGATCTTGCACTGCCCTTGGAAACAAAAGTAGCAACA GTTCCAATTAAAGACAAAGGAACTGTACAGACTGAAGAAAAACCACGTGAAGACTCCCAGTTAGCATCTA TGCAGCACAAGGGACAGTCAACAGTACCTCCTTGCACGGCTTCACCAGAACCAGTCAAAGCTGCAGAACA AATGTCTACCTTACCAATAGATGCACCTTCTCCATTAGAGAACTTAGAGCAGAAGGAAACGCCTGGCAGC CAGCCTTCTGAGCCTTGCTCAGGAGTATCCCGGCAAGAAGAAGCAAAGGCTGCTGTAGGTGTGACTGGAA ATGACATCACTACCCCGCCAAACAAGGAGCCACCACCAAGCCCAGAAAAGAAAGCAAAGCCTTTGGCCAC CACTCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTCTCCCTAAGCAACCAGCT CCCACCACCTCTGGTGGGTTGAATAAAAAACCCATGAGCCTCGCCTCAGGCTCAGTGCCAGCTGCCCCAC ACAAACGCCCTGCTGCTGCCACTGCTACTGCCAGGCCTTCCACCCTACCTGCCAGAGACGTGAAGCCAAA GCCAATTACAGAAGCTAAGGTTGCCGAAAAGCGGACCTCTCCATCCAAGCCTTCATCTGCCCCAGCCCTC AAACCTGGACCTAAAACCACCCCAACCGTTTCAAAAGCCACATCTCCCTCAACTCTTGTTTCCACTGGAC CAAGTAGTAGAAGTCCAGCTACAACTCTGCCTAAGAGGCCAACCAGCATCAAGACTGAGGGGAAACCTGC TGATGTCAAAAGGATGACTGCTAAGTCTGCCTCAGCTGACTTGAGTCGCTCAAAGACCACCTCTGCCAGT TCTGTGAAGAGAAACACCACTCCCACTGGGGCAGCACCCCCAGCAGGGATGACTTCCACTCGAGTCAAGC CCATGTCTGCACCTAGCCGCTCTTCTGGGGCTCTTTCTGTGGACAAGAAGCCCACTTCCACTAAGCCTAG CTCCTCTGCTCCCAGGGTGAGCCGCCTGGCCACAACTGTTTCTGCCCCTGACCTGAAGAGTGTTCGCTCC AAGGTCGGCTCTACAGAAAACATCAAACACCAGCCTGGAGGAGGCCGGGCCAAAGTAGAGAAAAAAACAG AGGCAGCTACCACAGCTGGGAAGCCTGAACCTAATGCAGTCACTAAAGCAGCCGGCTCCATTGCGAGTGC ACAGAAACCGCCTGCTGGGAAAGTCCAGATAGTATCCAAAAAAGTGAGCTACAGCCATATTCAATCCAAG TGTGGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGCAATGTTCAGATTCAGAACAAGAAAGTGG ACATATCCAAGGTCTCCTCCAAGTGTGGGTCCAAAGCTAATATCAAGCACAAGCCTGGTGGAGGAGATGT CAAGATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAAGCCAAAGTGGGATCCCTTGATAACGTG GGCCACTTGCCTGCAGGAGGTGCCGTGAAGACTGAGGGCGGTGGCAGTGAGGCCCTTCCGTGTCCAGGCC CCCCCGCTGGGGAGGAGCCAGTCATCCCTGAGGCTGCGCCTGACGCTGGCGCCCCTACTTCAGCCAGTGG CCTCAGTGGCCACACCACCCTGTCAGGGGGTGGTGACCAAAGGGAGCCCCAGACCTTGGACAGCCAGATC CAGGAGACAAGCATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205330
Insert Size	3378 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205330.1, NP_001192259.1
RefSeq Size	5776 bp
RefSeq ORF	3378 bp
Locus ID	17758
UniProt ID	P27546
Gene Summary	Non-neuronal microtubule-associated protein. Promotes microtubule assembly.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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