Rbl2 (NM_001282001) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	p130; PRB2; Rb2; RBR-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229393 representing NM_001282001 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTGGAGGCAACCAGTCGCCACCGCCTCCTCCAGCTGCTGCAGCCAGCTCGGAGGAAGAGGAGG AGGATGGCGACGCCGCGGATCGCGCGCAGCCCGCGGGGTCCCCGAGCCATCAGATCCAGCAGCGGTTCGA GGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGCGCCGAGGCCTGGAGCAGCTACCGCAGCATG AGCGAGAGCTACACGCTGGAGGGAAATGACCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAA AATCTGTTCCAACTGTGAGCAAAGGGACCGCTGAAGGAAACTATGTATCTTTAACCAGAATCCTTCGCTG TTCGGAGCAGAGCCTAATTGAATTTTTTAACAAGATGAAGAAGTGGGAAGACATGGCAAATCTGCCCCCA CATTTCCGAGAACGTACTGAAAGATTAGAAAGAAACTTCACTGTTTCTGCTGTGATTTTTAAGAAATATG AACCCATTTTTCAAGACATTTTTAAATATCCCCAAGAAGAACAGCCTCGCCAGCAAAGAGGAAGAAAACA GAGGCGACAGCCCTGTACCACATCAGAAATTTTCCATTTTTGCTGGGTGCTTTTTATATATGCGAAAGGG AACTTCCCCATGATTAGCGATGATCTGGTCAATTCCTACCATCTTCTGCTGTGCGCATTAGATTTAGTCT ATGGAAATGCCCTTCAGTGTTCTAACCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCCTGTCCGAGGA CTGTCACCCCAAGGACTCTAAAGCGTCCTCCGACCCGCCGTGTGTCATTGAGAAGCTGTGCTCCTTACAC GACGGTCTAGTGTTGGAGGCCAAGGGGATAAAGGAACACTTCTGGAAACCCTATATTAGGAAACTGTTTG AGAAAAAGCTTCTCAAGGGGAAGGAAGAAAATCTTACTGGCTTCCTGGAGCCCGGAAACTTTGGAGAGAG TTTTAAGGCCGTTAATAAGGCATATGAAGAATACGTGTTAGCCGCTGGGAATCTGGATGAGCGCGTATTC CTTGGTGAGGATGCTGAGGAGGAAGTTGGGACTCTGTCTCGGTGTCTAAGTGCTGCCTCAGGTACAGAGA GTGCTGAACGGACGCAGATGAGAGACATCTTGCAGCAGCATCTTGACAAGTCTAAAGCACTTAGAGTCTG CACACCACTGACTGGCGTGAGGTATGTTCAGGAGAACAGCCCGTGTGTGACTCCCGTCTCCACAGCTGCA CACAGCCTGAGCCGTCTTCACACCATGCTGTCCGGCCTCAGGAATGCACCCAGTGAGAAGCTGGAGCGGA TACTCAGGTCATGTTCCCGAGATCCAACTCAGGCTATCGCTGACAGATTGAAAGAAATGTACGAAATATA TTCTCAGCATTTCCAGCCAGATGAGAATTTTAGTAATTGTGCTAAAGAAATGCTCTACTATAAAGTATTA GAGTCTGTTATTGAGCAAGAACAAAAAAGATTGGGAGACATGGATTTATCTGGTGTTCTGGAGCATGACG CATTCCACAGGTCACTCTTGGCCTGCTGCCTTGAGGTGGTCGCTTTTTCCCATAAGCCTCCTGGGAATTT TCCATTTATTGCTGAAATATTTGATGTACCACATTATCATTTTTATAAGGTAATTGAAGTATTTATTAGA GCAGAAGACGGTCTTTGCAGAGAAGTGGTCAAACACCTCAATCAGATTGAAGAACAAATTTTAGACCATT TGGCATGGAAAACCAAGTCCCCACTGTGGGACAGAATTAGAGATAATGAAAACAGAGTCCCTACTTGTGA AGAGGTCATGCCACCTCAAAACCTAGAGAGAACAGATGAAATTTACATCGCTGGCTCTCCCTTAACCCCG AGAAGGGTGGGTGAAGTTCGTGCTGATGCTGGAGGACTTGGAAGAAGTATAACGTCTCCAACCACATTGT ATGACAGGTACAGCTCCCCAACAGTCAGCACTACTAGAAGGCGGCTATTCGAGAATGATAGTCCCTCTGA AGGAAGCACATCTGGGCGCATCCCCCCACAACCCCTAGTCAACGCTGTCCCCGTGCAGAATGTACCTGGG GAGACTGTTTCTGTCACACCAGTTCCTGGACAGACCTTGGTCACCATGGCAACAGCCACTGTCACGGCCA ACAATGGACAAACAGTGACCATTCCAGTCCAAGGTATTGCCAACGAAAATGGAGGGATAACCTTCTTCCC AGTCCAAGTCAACGTTGGGGGCCAGGCCCAGGCTGTCGCTGGCTCTATCCAGCCCCTCAGTGCTCAAGCA CTGGCTGGAAGTCTGAGTTCCCAACAGGTGACAGGAACCACTTTGCAAGTCCCTGGTCCGGTGGCCATTC AACAGATTTCCCCTGGTGGACAACAGCAGAACCCAGGCCAGCCACTAACCAGCAGCAGTATCCGGCCGCG GAAGACTAGCTCCTTAGCGCTCTTCTTTAGAAAGGTTTACTACTTAGCCGGTGTCCGCCTTCGAGATCTT TGTATAAAACTAGATATTTCAGATGAACTGAGGAAAAAAATTTGGACCTGCTTTGAATTCTCTATAATCC AGTGCACCGAACTTATGATGGACAGACATCTGGACCAGCTGTTGATGTGTGCCATTTATGTGATGGCAAA GGTCACAAAAGAAGACAGGTCCTTCCAGAACATCATGCGTTGTTACAGAACTCAGCCACAGGCCCGGAGC CAGGTGTACAGAAGTGTCTTGATAAAAGGGAAAAGAAGAAACTCTGGCAGCAGTGAGAGCAGAAGCCATC AGAATTCTCCAACCGAACTAAATACAGACAGAGCCAGTAGAGATTCCAGCCCAGTGATGAGGTCAAACAG CACCCTACCAGTTCCACAGCCCAGCAGTGCCCCTCCTACACCAACTCGACTCACGGGTGCCAGCAGTGAC GTTGAAGAGGAGGAACGAGGAGACCTCATTCAGTTCTACAACAACATCTATAGGAAGCAAATCCAAGCGT TTGCCATGAAGTACTCGCAGGCAAACGCGCAGACGGACACTCCTCCCCTCTCTCCCTATCCATTTGTAAG AACAGGCTCCCCTCGCCGAGTACAGTTATCTCAAAGTCATCCTATCTACATTTCCCCACATAACAACGAA GCAATGCCTTCTCCTCGAGAGAAGATTTTTTACTACTTCAGCAACAGCCCATCAAAGAGACTGAGGGAAA TCAACAGTATGATACGGACAGGAGAGACTCCAACTAAAAAGAGAGGGATTCTCTTGGACGACGGAAGTGA ATCACCTGCAAAAAGAATCTGCCCAGAGAATCACTCTGCTCTGTTACGTCGTCTCCAGGATGTGGCGAAT GACCGAGGTTCACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282001
Insert Size	3378 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282001.1, NP_001268930.1
RefSeq Size	4905 bp
RefSeq ORF	3378 bp
Locus ID	19651
UniProt ID	Q64700
Gene Summary	Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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