Jak2 (NM_008413) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fd17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229400 representing NM_008413 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATGGCCTGCCTTACAATGACAGAAATGGAGGCAACCTCCACATCTCCTGTACATCAGAATGGTG ATATTCCTGGAAGTGCTAATTCTGTGAAGCAGATAGAGCCAGTCCTTCAAGTGTATCTGTACCATTCTCT TGGGCAAGCTGAAGGAGAGTATCTGAAGTTTCCAAGTGGAGAGTATGTTGCAGAAGAAATTTGTGTGGCT GCTTCTAAAGCTTGTGGTATTACGCCTGTGTATCATAATATGTTTGCGTTAATGAGTGAAACCGAAAGGA TCTGGTACCCACCCAATCATGTCTTCCACATAGACGAGTCAACCAGGCATGACATACTCTACAGGATAAG GTTCTACTTCCCTCATTGGTACTGTAGTGGCAGCAGCAGAACCTACAGATACGGAGTGTCCCGTGGGGCT GAAGCTCCTCTGCTTGATGACTTTGTCATGTCTTACCTTTTTGCTCAGTGGCGGCATGATTTTGTTCACG GATGGATAAAAGTACCTGTGACTCATGAAACTCAGGAAGAGTGTCTTGGGATGGCGGTGTTAGACATGAT GAGAATAGCTAAGGAGAAAGACCAGACTCCACTGGCTGTCTATAACTCTGTCAGCTACAAGACATTCTTA CCAAAGTGCGTTCGAGCGAAGATCCAAGACTATCACATTTTAACCCGGAAGCGAATCAGGTACAGATTTC GCAGATTCATTCAGCAATTCAGTCAATGTAAAGCCACTGCCAGGAACCTAAAACTTAAGTATCTTATAAA CCTGGAAACCCTGCAGTCTGCCTTCTACACAGAACAGTTTGAAGTAAAAGAATCTGCAAGAGGTCCTTCA GGTGAGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGGTCAAGAGGGAAACATA AGGAAAGTGAGACACTGACAGAACAGGACGTACAGTTATATTGTGATTTCCCTGATATTATTGATGTCAG TATTAAGCAAGCAAACCAGGAATGCTCAAATGAAAGTAGAATTGTAACTGTCCATAAACAAGATGGTAAA GTTTTGGAGATAGAACTTAGCTCATTAAAAGAAGCCTTGTCATTCGTGTCATTAATTGACGGGTATTACA GACTAACTGCGGATGCGCACCATTACCTCTGCAAAGAGGTGGCTCCCCCAGCTGTGCTCGAGAACATACA CAGCAACTGCCACGGCCCAATATCAATGGATTTTGCCATTAGCAAACTAAAGAAGGCGGGTAACCAGACT GGACTATATGTGCTACGATGCAGCCCTAAGGACTTCAACAAATACTTTCTGACCTTTGCTGTTGAGCGAG AAAATGTCATTGAATATAAACACTGTTTGATTACGAAGAATGAGAATGGAGAATACAACCTCAGCGGGAC TAAGAGGAACTTCAGTAACCTTAAGGACCTTTTGAATTGCTACCAGATGGAAACTGTGCGCTCAGACAGT ATCATCTTCCAGTTTACCAAATGCTGCCCCCCAAAGCCAAAAGATAAATCAAACCTTCTCGTCTTCAGAA CAAATGGTATTTCTGATGTTCAGATCTCACCAACATTACAGAGGCATAATAATGTGAATCAAATGGTGTT TCACAAAATCAGGAATGAAGATTTAATATTTAATGAAAGTCTTGGCCAAGGTACTTTTACAAAAATTTTT AAAGGTGTAAGAAGAGAAGTTGGAGATTATGGTCAACTGCACAAAACGGAAGTTCTTTTGAAAGTCCTAG ATAAAGCACATAGGAACTATTCAGAGTCTTTCTTCGAAGCAGCAAGCATGATGAGTCAGCTTTCTCACAA GCATTTGGTTTTGAATTATGGTGTCTGTGTCTGTGGAGAGGAGAACATTCTGGTTCAAGAATTTGTAAAA TTTGGATCACTGGATACATACCTGAAGAAGAACAAAAATTCCATAAATATATTATGGAAACTTGGAGTGG CTAAGCAGTTGGCATGGGCCATGCATTTTCTAGAAGAAAAATCCCTTATTCATGGGAATGTGTGTGCTAA AAATATCCTGCTTATCAGAGAAGAAGACAGGAGAACGGGGAACCCACCTTTCATCAAACTTAGTGATCCT GGCATTAGCATTACAGTTCTACCGAAGGACATTCTTCAGGAGAGAATACCATGGGTACCTCCTGAATGCA TTGAGAATCCTAAAAATCTCAATCTGGCAACAGACAAGTGGAGCTTCGGGACCACTCTGTGGGAGATCTG CAGTGGAGGAGATAAGCCCCTGAGTGCTCTGGATTCTCAAAGAAAGCTGCAGTTCTATGAAGATAAGCAT CAGCTTCCTGCACCCAAGTGGACAGAGTTAGCAAACCTTATAAATAATTGCATGGACTATGAGCCAGATT TCAGGCCTGCTTTCAGAGCTGTCATCCGTGATCTTAACAGCCTGTTTACTCCAGATTATGAACTACTAAC AGAAAATGACATGCTACCAAACATGAGAATAGGTGCCCTAGGGTTTTCTGGTGCTTTTGAAGACAGGGAC CCTACACAGTTTGAAGAGAGACACTTGAAGTTTCTACAGCAGCTTGGCAAAGGTAACTTCGGGAGTGTGG AGATGTGCCGCTATGACCCGCTGCAGGACAACACTGGCGAGGTGGTCGCTGTGAAGAAACTCCAGCACAG CACTGAAGAGCACCTCCGAGACTTTGAGAGGGAGATCGAGATCCTGAAATCCTTGCAGCATGACAACATC GTCAAGTACAAGGGAGTGTGCTACAGTGCGGGTCGGCGCAACCTAAGATTAATTATGGAATATTTACCAT ATGGAAGTTTACGAGACTATCTCCAAAAACATAAAGAACGGATAGATCACAAAAAACTTCTTCAATACAC ATCTCAGATATGCAAGGGCATGGAATATCTTGGTACAAAAAGGTATATCCACAGGGACCTGGCAACAAGG AACATATTGGTGGAAAATGAGAACAGGGTTAAAATAGGAGACTTCGGATTAACCAAAGTCTTGCCGCAGG ACAAAGAATACTACAAAGTAAAGGAGCCAGGGGAAAGCCCCATATTCTGGTACGCACCTGAATCCTTGAC GGAGAGCAAGTTTTCTGTGGCCTCAGATGTGTGGAGCTTTGGAGTGGTTCTATACGAACTTTTCACATAC ATCGAGAAGAGTAAAAGTCCACCCGTGGAATTTATGCGAATGATTGGCAATGATAAACAAGGGCAAATGA TTGTGTTCCATTTGATAGAGCTACTGAAGAGCAACGGAAGATTGCCAAGGCCAGAAGGATGCCCAGATGA GATTTATGTGATCATGACAGAGTGCTGGAACAACAATGTGAGCCAGCGTCCCTCCTTCAGGGACCTTTCC CTTCGGGTGGATCAAATCCGGGACAGTATAGCTGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_008413
Insert Size	3399 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008413.3, NP_032439.2
RefSeq Size	5055 bp
RefSeq ORF	3399 bp
Locus ID	16452
UniProt ID	Q62120
Gene Summary	Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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