Nek1 (NM_001293639) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D8Ertd790e; kat
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229448 representing NM_001293639 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATGTGAGACTGCAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGA CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGA ATCAAGGAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATCATTT GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTTTAAACGAATAAATG CTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTTTGTGCAGATATGTTTGGCTCTGAA GCATGTACATGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGCACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTG GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC CTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTATGATCTCCGCAGCT TGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT TATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACACTT TCAAAGTTTGGACCACAGCCTCTCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC CTGCTCAGAAAATCACAAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAA AAAGTTACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAATGAAT TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGAATTTATTGAGAAAG AAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGATGAAGCGGCAAGAGAAGCAGCGGGC TTCCTTTTTTGGCATTGGAGGGGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAACATTAC CATGCCATTTTTGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGGAATCT ATGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGTGGAAGAATT CCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACACGTGGTTTATTTGGCAAGA CTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGAGAATA AAGAAGCTGATGGTACCAAAGGACAAGAAGCAACTGAAGAGACTGACATGAGGCTCAAAAAGATGGAGTC ACTTAAGGCGCAAACAAATGCACGTGCTGCTGTACTAAAAGAACAGCTGGAGCGAAAAAGAAAGGAAGCT TATGAAAGAGAAAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAAAGCTCAGATGTTCCTCTGC CTTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGCCTGTCATTTCTGTGACTTC AGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGAAGAAATGGAAAAGAGTAAC AGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAATGAAAATCTTAAAGCTCAAGAGGATGAAA AGGAAAAGCAGCATCACTCAGGTTCTTGTGAGACCGTTGGTCACAAAGATGAGAGAGAGTATGAGACAGA AAATGCCATTTCCTCTGATCGCAAGAAGTGGGAGATGGGAGGTCAGCTTGTGATTCCTCTCGATGCAGTG ACACTGGATACATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTTATTAAATTAGATTCTAATG GCTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGATACTTGGAGAAGCTGAATT ACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTATGCTGAAGAGGAGAACTAC AAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTCAAAAGAAATAAATCCATCAGCTACTGTTG ATTCTACTGAAACGAAAAGTCCAAAGTTTACTGAGGTGTCTCCACAAATGTCAGAAGGAAATGTGGAAGA ACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTAGCACACACACAGATGGGAGTTTGCCACCT GTTCTTAATGATGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAGTTGGAAGATAAGGTTGCTG TGCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAATCCTGTAAGGATCAGAGAGA TCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGTACAGCCAGAATCGATTTTC CAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGCAGTTCATTGCTCACCAGAAGAACCAATTC CAATTCGATCTCACTCTGATTCTCCACCAAAAACTAAGAGCAAGAATTCCTTACTGATTGGACTTTCAAC TGGTCTGTTTGATGCAAACAATCCAAAGATGCTGAGGACCTGCTCACTTCCAGATCTTTCCAAGCTGTTC AGAACCCTAATGGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTTGAAATCGATGAGCTGGAAG ATGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAGAAACTGACACAGATTTACA AGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGAATACAGTGAGGAGGAAGAG TCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGGGACAGATGCCCCAGACGAGGAGGACAACC CCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGATAATAGTGACGCTGAGACCACTAGTGAATG TGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAGCAAGAAATGGGCTTTGAA AAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAATTTGTT CAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCAAGATTCTGCATTTAGTCAT GGCAGATGGAGCCTATCAGGAAGATAATGATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293639
Insert Size	3537 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293639.1, NP_001280568.1
RefSeq Size	5344 bp
RefSeq ORF	3537 bp
Locus ID	18004
UniProt ID	P51954
Gene Summary	Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks three alternate in-frame exons, compared to variant 1. The encoded protein (isoform 4) is shorter, compared to isoform 1.

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