Ehmt2 (NM_001286575) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bat8; D17Ertd710e; G9a; KMT1C; NG36
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229469 representing NM_001286575 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGAGCTGCTGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGC TGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAGTTCATAGCTCTTTGGGGGACACCCCTCAGAG TGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGAGCCTGCCGGCCCCTCCTCTCCGGCCTCTGTC ACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTCGGGGCCGCATCACTCATCGGGGACGAACCCG AGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCCATGCCACAAAGTCGTTCCCCTCTTCCCCCAG CAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAATGACAGGGGCAGGAAAGTCGCCCCCCTCGGTC CAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCCCAGGGAGCTGCAACTGCTGGGCCTGAACCCT CTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAGTGCACCGAGCCCGGAAAACCATGTCCAAACC TAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCCTGAAGTCCAGCATTTCCGCATGAGTGATGAC ATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGGAGGAAGCTGAACTCTGGTAGCCTGTCCGAGG ACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGGAGAAGGGAGAGCCCAGGCCCCTGGAGGAGTG GGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGATGCGTACTCTGTGGATGAGCGGGTGGACTCT GACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTGAGTGAGGAGGAGGAGGAGGAAGAGGAGGAAG AAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGAGGACGAGGAGTCGGGCAATCAGTC AGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGGAAAGACAGCCCGTGGGTGAAG CCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCAAGAGGAGTGAATGGTGTGGGTT CCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGTCCCTGGAGCTGCCCAGCGAGGGGACCCTCTC CCCCAACCACGCTGGGGTCTCCAATGACACGTCTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCC CTCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGG CCACAGAGAGTGTGGATGGAGAGCTCCTGGGCTGCAATGCTGCCATCCTTAAGCGGGAGACCATGCGGCC GTCTAGCCGCGTGGCGCTGATGGTGCTCTGTGAGGCCCATCGAGCCCGCATGGTCAAGCACCATTGCTGC CCGGGCTGCGGCTACTTCTGCACAGCGGGCACCTTCCTGGAATGCCACCCCGACTTTCGTGTAGCTCACC GCTTCCATAAGGCCTGCGTATCCCAGCTCAATGGGATGGTCTTCTGTCCCCACTGTGGAGAGGATGCCTC AGAGGCCCAGGAGGTGACCATTCCTCGGGGCGATGGGGGAACACCCCCAATTGGCACCGCAGCTCCTGCT CTGCCACCCCTGGCACATGATGCCCCAGGGCGAGCGGATACCTCCCAGCCTAGCGCCCGAATGCGAGGGC ATGGAGAGCCGCGGCGCCCGCCCTGTGATCCCCTGGCTGACACCATCGACAGCTCAGGGCCTTCACTGAC TCTGCCTAATGGGGGCTGCCTCTCCGCTGTGGGTCTGCCCCCAGGGCCGGGCAGGGAAGCCCTGGAAAAA GCCTTGGTCATCCAGGAGTCTGAGAGGCGGAAGAAGCTGCGATTCCACCCACGGCAGCTGTACCTGTCGG TGAAGCAGGGGGAGCTGCAGAAGGTGATCCTTATGCTGTTAGACAACCTGGACCCCAACTTCCAGAGCGA CCAGCAGAGCAAGCGCACGCCCCTGCACGCGGCCGCCCAGAAGGGGTCGGTAGAGATCTGTCATGTGCTG CTGCAGGCAGGAGCCAACATCAATGCCGTAGATAAGCAACAACGCACGCCACTAATGGAGGCCGTGGTGA ACAACCACCTGGAGGTGGCACGCTACATGGTGCAGTTAGGTGGCTGTGTCTACAGCAAGGAAGAGGATGG CTCCACCTGTCTACATCATGCAGCCAAAATTGGGAACTTGGAAATGGTCAGCCTGCTACTGAGCACAGGA CAGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACGCCCATCATCTGGGCAGCCGAGCACAAGCACA TCGATGTGATTCGTATGCTGCTGACCCGGGGTGCCGATGTCACCCTGACTGACAATGAGGAAAACATCTG CCTGCACTGGGCCTCCTTCACGGGTAGTGCCGCCATCGCTGAGGTCCTTCTGAATGCCCAGTGTGATCTC CATGCTGTCAACTACCATGGGGACACGCCCCTGCACATAGCCGCCAGGGAGAGCTACCATGACTGTGTTC TGTTGTTCCTGTCTCGTGGAGCCAACCCTGAGCTTCGGAACAAAGAAGGAGACACGGCATGGGATCTGAC CCCAGAGCGCTCTGATGTGTGGTTTGCACTGCAGCTCAATCGAAAGCTTAGGCTTGGGGTAGGGAACCGG GCTGTCCGCACCGAGAAGATCATCTGCCGGGACGTAGCCCGAGGCTATGAGAATGTACCCATCCCCTGTG TCAATGGTGTGGATGGGGAGCCGTGCCCGGAGGACTACAAGTACATCTCTGAGAACTGCGAGACATCGAC CATGAACATCGACCGCAACATCACCCATCTGCAGCACTGCACGTGTGTGGATGACTGCTCCAGCTCCAAT TGCCTATGTGGTCAGCTCAGTATCCGATGCTGGTATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACA AGATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCATGCTCCTGCTGGAGAAGCTGCAAGAACCGCGT GGTGCAGAGCGGCATCAAGGTACGGCTGCAGCTCTACCGGACTGCCAAGATGGGCTGGGGGGTCCGAGCC TTGCAGACCATCCCCCAGGGCACGTTCATCTGCGAGTATGTAGGAGAGCTGATCTCTGATGCCGAGGCTG ATGTGAGAGAGGATGATTCTTACCTCTTCGATTTAGATAACAAGGATGGCGAGGTTTACTGCATTGATGC CCGTTACTATGGCAACATCAGCCGATTCATTAACCACCTGTGTGACCCCAACATCATCCCTGTCCGGGTT TTCATGCTGCACCAAGATCTACGGTTCCCACGCATTGCCTTCTTCAGCTCCAGGGACATCCGGACTGGGG AGGAGCTGGGCTTTGACTACGGTGACCGATTCTGGGACATCAAGAGCAAGTATTTCACCTGCCAGTGTGG CTCTGAGAAGTGCAAGCATTCAGCGGAGGCCATCGCCCTGGAGCAGAGCCGCCTGGCCCGGCTGGACCCC CACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCCATCAACACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286575
Insert Size	3621 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286575.1, NP_001273504.1
RefSeq Size	3907 bp
RefSeq ORF	3621 bp
Locus ID	110147
UniProt ID	Q9Z148
Gene Summary	Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) has an alternate 5' terminal exon, which includes the 5' coding sequence, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus, compared to isoform a.

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