Tyk2 (NM_001205312) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	JTK1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229470 representing NM_001205312 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGACCATGCCTCTGTGTGGGCGGAGAGCCATCTTGGAAGACAGCAAGGCAGATGGCACAGAAG CTCAGCCCCTGGTGCCCACAGGATGCTTGATGGTGCTACTGCATTGGCCTGGGCCTGAGGGCGGGGAGCC CTGGGTCACCTTCAGCCAGACATCTCTGACTGCAGAGGAGGTCTGCATCCACATCGCACACAAAGTCGGC ATCACTCCACCCTGCTTGAATCTCTTCGCACTCTACAATGCACAGGCTAAGGTCTGGCTGCCCCCAAACC ATATTCTGGATACATCCCAAGACATGAACCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTGGCATGG CATGAATCCCCAGGAGCCAGCTGTATACCGGTGTGGTTTCCCAGGGGCAGAGACTTCCTCAGACCGGGCA GAGCAAGGTGTACAGCTCTTGGACTCTGCCTCGTTTGAATACCTTTTTGAGCAGGGATGGTATGTTGGCA ATAGAGCAAAAAGCTCCCACACCTCTTTTTCTATCCATCGCTCTACCCCTCAGGGAAAGCATGAGTTCAT GAACGATGTGGTGTCTCTGCGGGACCTGTCTAGCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCTTA GGCATGGCCTTCCTGCACCTGTGTCACCTTGCTCTCAGCCGAGGCGTCCCCCTGGAGGAGATGGCCAGAG AGATCAGCTTCAAGAACTGCATCCCTCATTCCTTCCGACAGCACATCCGGCAGCACAATGTGCTCACACG CCTGCGGCTCCACAGAGTCTTCCGCCGCTTCCTGCGGGCCTTCCGGCCTGGCCACCTCTCCCAGCAGGTT GTGATGGTGAAGTACTTGGCTACCCTGGAGCGGCTGGCTCCGCGCTTTGGCTCAGAGCGCATACCTGTGT GTCATCTGGAGGTCCTGGCACAGCCCGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGACCGCTGG GGATCCAGGCCCAGAGCTGCCTTCCGGGCCTCCCACCCACGAGGTACTGGTGACAGGCACCGGAGGTATC CAGTGGCATCCGCTGCAGACCCAGGAATCTGAGAGAGGTAACAGCAGAGGGAATCCCCACGGCAGCCGGT CGGGGAAGAAGCCCAAAGCCCCCAAGGCTGGAGAGCACCTGACAGAGAGCCCTCAGGAACCACCTTGGAC CTACTTCTGTGACTTCCAGGACATTTCCCACGTGGTGCTAAAGGAGCGTCGCGTGCACATCCACCTCCAA GACAACAAGTGCTTGTTGCTGTGCCTCTGTTCCCAGGCTGAGGCCCTGTCCTTTGTGGCCCTGGTCGATG GCTATTTCCGCTTGACTGCTGACTCCAGCCACTATCTGTGCCATGAGGTGGCACCCCCGAGGCTGGTGAC TAGCATCCAGAACGGCATCCATGGGCCCCTGATGGATCCGTTTGTACAGGCCAAGCTGTGGCCAGAGGAC GGCCTCTACCTGATTCAGTGGAGCACCAGCCACCTGCACCGCCTGATCCTTACCGTGGCCCATCGAAACC CGGCTTTCAGTAATGGCCCTCGGGGCCTGCGCCTGCGAAAGTTCCCCATCACACAGCAGCCTGGAGCCTT TGTGCTGGATGGCTGGGGCCGCTCCTTTGCCAGCTTGGGGGACCTTCGGCTCGCCTTGCAGGGCTGCTCG TTGCGGGCCGGTGATGACTGTTTCCCCCTGCACCACTGCTGCCTGCCCCGGCCAAGAGAAATCTCCAACC TCGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCTCTCAACCTCAGTCAGCTCAGCTTCCACAGGGT TCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGAGGGCCTT CTAAGAGTGGGAGGCCCCGATGAGGGCAAAGTGGACAATGGATGTCCCCCTGAGCCTGGTGGGACTAGTG GGCAGCAGCTTCGAGTGGTGCTCAAAGTTCTCGACCCCAGTCACCATGATATCGCCTTGGCCTTCTATGA GACTGCTAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTTTGCGTGCGTGGC TCAGAGAATATCATTGTGACAGAATTCGTAGAACATGGTCCCTTGGATGTGTGGTTACGGCGACAGAGGG GCCAAGTGCCCATGACCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTCAGCTACCTGGAGGA CAAGAATCTGGTTCACGGGAATGTATGTGGCCGGAACATCCTGCTGGCTCGCCTGGGGTTGGAGGAGGGT ACCAACCCCTTCATCAAGCTAAGTGATCCTGGTGTGGGCCAGGGTGCCCTCTCCAGGGAAGAGCGGGTGG AGCGCATCCCCTGGACAGCTCCCGAGTGCCTGTCTGGAGGGACCAGTAGCTTGGGTACTGCCACGGACAT GTGGGGCTTTGGTGCCACCCTTCTTGAGATCTGCTTTGATGGGGAGGCACCCCTGCAGGGTCGTGGTCCC TCTGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCTGAACCCTCATCCCCAGAGCTGGCCACAC TCACCCGCCAGTGCCTGACCTATGAACCAGCACAGCGGCCATCCTTCCGCACCATTTTGCGGGACCTCAC AAGGCTGCAGCCACAGAATCTAGTCGGCACTTCGGCTGTGAACTCAGACTCACCAGCATCAGACCCCACT GTTTTCCACAAGCGCTATTTGAAAAAGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTCAGCCTGT ACTGCTACGACCCAACCAATGACGGCACTGGCGAGATGGTGGCCGTGAAGGCCCTGAAGGAAGGGTGCGG TCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAGATCCTGCGGACGCTGTACCACGAACATATTGTC AAGTATAAAGGCTGCTGTGAGGACCAAGGAGAGAAGTCTGTACAGCTGGTCATGGAGTACGTCCCTCTGG GCAGCCTCCGAGACTACCTGCCAAGGCACTGCGTAGGGCTGGCGCAGCTCCTGCTGTTTGCCCAGCAGAT CTGCGAGGGCATGGCCTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCCGCGCGCAACGTGCTG CTGGACAACGACAGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGCCACGAGT ACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTGTTCTGGTATGCCCCAGAATGCCTGAAGGAGTGCAA ATTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTAACTCTGTATGAGTTGTTGACATACTGTGACTCT AACCAGAGTCCCCATATGAAATTCACCGAGCTCATCGGCCATACCCAGGGCCAGATGACCGTGCTGAGGC TCACAGAGCTGCTGGAACGAGGAGAGAGGCTGCCTCGGCCTGACAGGTGTCCCTGTGAGATCTATCACCT CATGAAGAACTGCTGGGAGACAGAGGCCTCCTTCCGGCCCACCTTCCAGAATCTTGTGCCCATCCTCCAG ACAGCACAGGAGAAGTACCAAGGTCAGGTGCCTTCCGTGTTCAGCGTGTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205312
Insert Size	3624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205312.1, NP_001192241.1
RefSeq Size	4873 bp
RefSeq ORF	3624 bp
Locus ID	54721
Gene Summary	Involved in intracellular signal transduction by amplifying type I and type II IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. Plays an essential role in promoting selective immune responses, including innate host defense mechanisms and specific antiviral activities.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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