Map4k4 (NM_001252201) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430080K19Rik; AU043147; AU045934; AW046177; HGK; Nik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229484 representing NM_001252201 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACGACTCTCCCGCGAAGAGCCTGGTGGACATTGACCTGTCGTCCCTGCGGGACCCTGCTGGGA TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC GGGTCAGCTGGCCGCCATCAAGGTTATGGACGTCACCGAGGATGAAGAGGAAGAAATCAAACTGGAGATA AATATGCTGAAGAAGTATTCTCATCATCGAAATATTGCCACGTACTATGGTGCTTTCATTAAGAAGAGCC CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTTTGTGGGGCTGGGTCCATCACAGACCTTGT GAAGAACACCAAAGGGAACACTCTCAAAGAAGACTGGATTGCTTACATCTCCAGGGAAATCCTCAGGGGA TTGGCACATCTCCATATTCACCACGTTATTCACCGAGATATCAAGGGCCAAAATGTGCTGCTGACCGAGA ATGCTGAGGTGAAACTTGTTGATTTTGGTGTAAGCGCTCAGCTGGACAGGACGGTTGGACGGAGAAATAC GTTCATAGGCACACCCTACTGGATGGCTCCAGAGGTCATCGCCTGTGATGAGAACCCAGACGCCACTTAC GACTACAGAAGTGACCTCTGGTCCTGTGGCATCACAGCCATCGAGATGGCTGAAGGGGCCCCCCCTCTCT GTGACATGCATCCAATGAGAGCGCTGTTTCTCATCCCCAGAAACCCTCCTCCCAGGCTGAAGTCAAAAAA ATGGTCAAAGAAATTTTTCAGCTTTATAGAAGGCTGTCTGGTGAAGAATTACATGCAGCGGCCCTCTACA GAGCAACTTTTAAAACACCCTTTCATAAGGGATCAGCCCAATGAAAGGCAGGTTCGAATCCAGCTTAAGG ATCACATAGACCGGACCAGAAAGAAGAGAGGCGAGAAAGATGAGACGGAGTACGAGTACAGCGGGAGCGA GGAGGAGGAGGAGGAAGTGCCTGAGCAGGAGGGAGAGCCAAGTTCCATCGTCAATGTGCCTGGAGAGTCA ACTCTGCGACGTGATTTCCTGAGACTGCAGCAGGAGAACAAGGAGCGGTCTGAGGCTCTGCGGAGACAGC AGCTTCTGCAGGAGCAGCAGCTCCGGGAGCAGGAGGAGTATAAGAGGCAGCTGCTGGCTGAGAGGCAGAA GCGGATTGAACAGCAGAAAGAACAGAGGAGGCGGCTGGAAGAGCAACAAAGAAGAGAACGGGAAGCCAGG AGGCAGCAGGAGCGTGAGCAGCGGCGGCGTGAACAAGAGGAGAAGAGGCGTCTCGAGGAACTGGAAAGGC GGCGTAAAGAAGAGGAAGAGAGGAGACGGGCAGAAGAGGAGAAGAGGAGAGTGGAGAGGGAACAGGAGTA CATCAGGCGGCAGCTAGAGGAGGAGCAGCGGCACCTGGAGATCCTGCAGCAGCAGCTGCTCCAGGAGCAG GCCATGTTACTGCACGACCACAGGAGGCCGCACGCACAGCAGCAGCCGCCGCCCCCGCAGCAGCAGGACA GGAGCAAACCGAGCTTTCATGCTCCAGAGCCCAAGCCTCACTATGACCCTGCTGACAGAGCTCGGGAGGT TCCCGTGAGAACGACCTCTCGTTCCCCTGTCCTCTCCCGTCGGGATTCCCCACTGCAGGGCGGTGGACAG CAGAATAGCCAAGCAGGACAGAGAAATTCCACCAGCAGTATTGAGCCCCGGCTTCTTTGGGAGAGAGTGG AAAAGCTGGTCCCCAGGCCAGGCAGTGGCAGCTCTTCAGGGTCCAGCAACTCAGGATCCCAGCCTGGCTC CCATCCTGGGTCTCAGAGCGGCTCCGGTGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGTTCT CCATCGCCACGCCAGGAAAGTGCAGCCAAAAAGCCTGACGATAAGAAAGAAGTATTCCGGCCTCTCAAGC CTGCGGGAGAAGTGGACTTGACTGCGTTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTTCGGCCACCTCA CAAAGTAACAGACTACTCATCCTCCAGTGAGGAGTCTGGGACCACAGATGAGGAGGAGGAGGACGTGGAA CAGGAGGGGGCTGATGATTCTACCTCGGGACCAGAAGACACCAGAGCAGCGTCATCTCCGAACCTGAGCA ACGGTGAAACAGAATCTGTGAAAACAATGATCGTTCATGATGATGTAGAAAGTGAGCCAGCCATGACCCC GTCCAAGGAGGGCACCCTCATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGCCAGCCATCATGAGAGC AATGGCTTTGCGGGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCT CCACCTCTTCCTCCCCATCCTCCAGCCAGCCGACACCCACCATGTCCCCACAGACACCCCAGGACAAGCT CACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCT TTTATAGACCCCAGGTTACTACAGATCTCTCCATCTAGTGGGACGACAGTGACTTCCGTGGTGGGATTTT CCTGTGATGGGCTGAGACCAGAAGCCATAAGGCAAGATCCTACTCGGAAAGGCTCAGTGGTCAATGTGAA CCCCACGAACACTAGGCCACAGAGTGATACCCCCGAGATTCGTAAATACAAGAAGAGATTTAACTCAGAG ATCCTGTGTGCTGCCTTATGGGGAGTGAACTTGCTGGTTGGTACAGAGAGCGGTCTGATGCTCTTGGACA GAAGTGGCCAAGGGAAAGTATACCCTCTGATCAGCCGAAGACGGTTCCAGCAAATGGATGTGCTCGAAGG CCTAAATGTCCTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTATCCTGGCTAAGA AATAAGATTCTTCATAATGATCCAGAGGTTGAGAAGAAGCAGGGGTGGACCACTGTGGGCGACTTGGAAG GGTGCGTGCACTATAAAGTCGTAAAATATGAAAGAATCAAGTTTCTGGTAATTGCTTTGAAGAGTTCTGT GGAAGTCTATGCATGGGCACCGAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAACTGCTA CATAAGCCATTACTGGTGGATCTCACTGTGGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTG CGGGATTCCATGCTGTTGATGTGGATTCAGGCTCAGTCTATGACATTTATCTACCAACACATATTCAGTG TAGCATCAAACCCCATGCAATCATTATCCTCCCCAACACGGATGGGATGGAGCTGCTGGTGTGCTACGAA GACGAGGGGGTTTACGTTAACACGTACGGAAGGATCACCAAGGACGTGGTTCTGCAGTGGGGAGAGATGC CGACATCTGTAGCATATATCCGGTCGAATCAGACAATGGGCTGGGGAGAGAAGGCAATAGAGATACGATC TGTGGAAACTGGTCACTTGGATGGTGTATTTATGCACAAAAGGGCTCAGAGACTAAAGTTCCTGTGTGAA CGCAATGACAAGGTGTTCTTTGCCTCCGTCCGCTCTGGTGGCAGCAGCCAGGTCTATTTCATGACCTTAG GCAGGACTTCTCTTCTGAGCTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252201
Insert Size	3666 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252201.1, NP_001239130.1
RefSeq Size	5581 bp
RefSeq ORF	3666 bp
Locus ID	26921
UniProt ID	P97820
Gene Summary	Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences in the presence and absence of in-frame exons in the central coding region, compared to variant 1, and encodes a shorter isoform (3), compared to isoform 1.

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