Dhx30 (NM_001252683) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810477H02Rik; C130058C04Rik; Ddx30; HELG; Ret-CoR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229485 representing NM_001252683 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCCAGGCGGCTCATGGCGCTGGCTGCCGGCGTCTCTCCGCGCCTCCGGCCGCCGGATCCCC TTGTCGCTTCCGGGCGACAAGGATGTTCGCGCGGCTTCTCATCAAGCTTTGTCCGCTCTGATGGCACTCA GGAAGCTGCCGAGGTCGAATCAGAGGTGGCCCCTAGCGAGCCTGGGGAGGGTGACGGAAGCATGGTGAAC GCTTCTAGGGACCTATTAAAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCC TTGGCATCTCACATGCAAAAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCAC CCTGCACATAAAGTGGCCCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGT CAGGCTGCAGCAGCTGCCTGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTG ATGCAGCTAAATACCGAGTGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACC CACCATGCCTCCAACTTCCTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTA TCCCGATCCTTAGGCCGAGAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGA CAGAGTTTCTGTCTATGACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGA AATGACAGATGATGACAGTGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAA GTGATTCAGATTGCAACCTCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGA CCAAGCTGGCTACACTCACTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGC TGAGGCTGAGAATAAGGCAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAAC AATGAGCCGCTTACCCATGCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGC CATGTACCATCCAGGTGCCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGA CAGCTCATGGATTTCCCCAGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGG CCCTTGAGTGACCCTATCACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCC AGAGCCTGCTAGAGCTGTGGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCC TCATCGGGACACTATCCTCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGT GGGAAGACCACACGTATCCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCA ATGTGATCATCACACAACCTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGG CCCCTCCTTGCGCCGGAATGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCG CTGCTCTTCTGCACTGTGGGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCC ATGTCATTGTGGATGAGGTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCT GCAGCGGCTCAACCCAGCTCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGA TACTTTGGGGGCTGCCCTGTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGG ACATCTTGGCCAAGCTGGGTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATG TGCACTTGATTTGGACCTTGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATC CTGTGCTTTCTACCTGGCTGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGC ATGAAAGCAAGTACCTCATCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCA ACAGCCTCCACTTGGGGTACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAAC GACATTGTACATGTCGTGGACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCT GCCTGGAGACTGTGTGGGTGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTC AGGTTTTGCCTACCACTTGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATC CTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGT TCCTCTCTAAGGCTGTGGACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGAT TGGGGTGCTGGACCAGCGGGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCC CGACTGGCCAAGGCCATAGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCT GCCTTACCCGGGACCCCTTCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCT GAGCCATGACAGTGGCAGTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGC TGGCAGGACCGTACCTCCAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCA TCCACGGGCTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTG CACACTGCCCTCTGCTCAGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCT GGCCTCTACCCCAACCTCATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACA GTGTCACTTACAGGACCAAATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCG GTTACGGAGCCGATGGCTGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCC TCCCAGGTGCACCCACTAGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTC GGGCCACCATCTCATTGAGTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCT AAGGGAGTTTCGTCGAGCCCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCT CTTAGTGTGCAGCAAGAACACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCA GCTTTGATATGCGCAAGACAGCTGATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252683
Insert Size	3672 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252683.1, NP_001239612.1
RefSeq Size	3807 bp
RefSeq ORF	3672 bp
Locus ID	72831
UniProt ID	Q99PU8
Gene Summary	RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.