Nfat5 (NM_001286260) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI225870; B130038B15Rik; CAG-8; CAG80; mKIAA0827; NFATL1; nfatz; OREBP; TonEBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229487 representing NM_001286260 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC GAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGTAGCATC AATGAGTCAGACAAGCGGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCTGATGCTTCTTCA GCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATT CTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAG AGGGGTAAGTGAAAAGCAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACA GTTTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGT GTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAG CACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCG GGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTC ACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAG CCAAAAGGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAG GAGCTGAAGATAGTGGTACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCT CTGTAAAAGACAGAACACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTT GCAGGTATTTGTGGGCAATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACT GGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATC CTAGCAACAACATGACACTGGCGGTGGACTGTGTTGGAATATTGAAGCTGAGGAATGCCGATGTTGAAGC CAGAATTGGAATTGCTGGATCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACA AGGAAAGATGGCTCTACTCTGACATTGCAGACACCTTCTTCCCCCATTTTATGCACTCAGCCAGCAGGAG TTCCTGAGATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTATTTTTAATTGG GAAAAACTTTCTGAAAGGAACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCA GAAGCTGAAATTGACATGGAGTTATTCCATCAGAACCATCTTATTGTGAAGGTTCCTCCGTATCATGACC AACATATAACTTTGCCTGTATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCA GCCATTTACGTACACTCCGGATCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAAAAGGAGATATCTAGT CCAGCAAGACCTTGCTCTTTTGAAGAAGCCATGAAAGCAATGAAAACGACTGGATGTAACGTAGATAAGG TGACTATCCTTCCTAATGCCCTGATCACTCCACTCATATCAAGCAGTATGATTAAGACTGAAGATGTTAC TCCAATGGAAGTAACTTCAGAAAAAAGATCTTCCCCAATTTTTCAGACTACAAAGAGCATTGGATCAACC CAGCAAACCTTAGAAACTATTTCTAACATAGCGGGGGGTGCACCCTTCTCCTCACCGTCGTCATCTTCCC ACTTAACTCCTGAAAGTGAGAATCAGCAGCAGCTTCAGCCCAAGGCATACAACCCAGAGACCCTGACAAC TATCCAGACACAGGACATCTCACAGCCCGGGACCTTTCCCGCAGTTTCTGCTGCTAGTCAGCTGCCCAGC AGCGATGCACTACTGCAGCAGGCAACACAGTTTCAGACAAGAGAAGCTCAGTCCAGAGACACAATACAGT CAGATACAGTGGTTAACTTGTCACAGTTAACTGAAGCATCACAGCAGCAGCAATCCCCACTACAAGAACA AGCACAAACGTTACAGCAGCAGATACCATCAAATATTTTTCCATCGCCAAGCAGTGTCAGCCAGCTCCAG AGTACTATTCAGCAGCTGCAAGCAGGAAGTTTCACAGGCAGCGCTGCTGGCGGCAGGAGTGGAAGCGTTG ATTTGGTCCAGCAGGTTTTAGAGGCACAACAGCAGTTATCGTCTGTTCTGTTTTCTACTCCAGATGGGAA TGAGAATGTTCAAGAACAGCTTAATGCAGACATTTTCCAAGTCAGTCAAATTCAAAATAGTGTAAGCCCT GGAATGTTTTCTTCAGCGGAGTCTGCAGTTCACACTAGACCAGATAACTTACTACCTGGGAGGGCTGACA GCGTCCATCAACAGACTGAAAATACACTGTCTAATCAGCAGCAGCAGCAACAGCAGCAGCAGCAAGTGAT GGAGTCATCAGCTGCAATGGTGATGGAGATGCAGCAGAGCATTTGCCAAGCAGCTGCCCAGATCCAGTCA GAGCTGTTTCCTTCAGCTGCTTCAGCAAGTGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTC ACATGATGAGTGCACTGCCTACCAACGAGGACATGCAGATGCAGTGTGAGCTGTTCTCGTCTCCCCCTGC AGCTTCTGGAAACGAAACTTCCACAACCACCACGCCACAGGTGGCAACCCCTGGCTCCACCATGTTTCAG ACACCAAGTTCAGGAGATGGAGAAGAAACTGGAGCACAAGCAAAACAGATTCAGAACAGCGTCTTTCAGA CAATGGTCCAAATGCAGCGCAGTGGTGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAATAT AATGAGTGTTCAGAATAATGGTACCCAGCAACAAGGGAATAGCTTGTTCCAGCAAGGGAGCGAGATGATG TCACTTCAGTCTGGGAACTTTTTGCAGCAGTCCTCTCATTCACAGGCTCAGCTCTTTCATCCTCAAAATC CTATTGCTGATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCAATTTTTCATAGTCCAAATCCTATTGT CCACAGTCAAACTTCCACAGCATCCTCTGAACAACTGCAGCCTTCAATGTTTCACTCTCAGAATACCATT GCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCACCCAACATATTTCTTTCCCAAAGTTCTA TCAATAATCTTCAAACTAACACAGTAGCCCAAGAAGAGCAGATTTCATTTTTTGCAGCACAGAACTCAAT TTCTCCACTTCAGTCAACATCAAACACTGAGCAGCAAGCTGCTTTCCAACAGCAGCCTCCAATCTCACAC ATCCAGACCCCTATTCTTTCCCAGGAACAGGCACAACCCTCTCAGCAAGGGTTATTTCAGCCTCAGGAAA GCTTACACTCACACATAACCCCAGACGCTTGCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286260
Insert Size	3678 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286260.1, NP_001273189.1
RefSeq Size	4725 bp
RefSeq ORF	3678 bp
Locus ID	54446
UniProt ID	Q9WV30
Gene Summary	Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (c) uses an alternate splice site and lacks a portion of the 3' coding region, compared to variant b. This results in a shorter protein (isoform c) with a distinct C-terminus, compared to variant b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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