Kcnma1 (NM_001253367) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229490 representing NM_001253367 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACAT CCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCC CAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGA TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTC CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATG ATAGCCATTGAGTACAAGTCTGCCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGA TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG CAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTTGAAGAT GAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGCCCAACACCT CCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAA TGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAAGTCATCTTG ACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGGATGTCAGCT CAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCATGAGCTCAA ACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAACTTCCCGAAA GTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACA TGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTT GGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGA TTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGT CCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTCGTGAATGATACCAATGTTCAGTTTTTGGA CCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTT GCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGA CCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGAGAATGCACTTCGAGGAGG CTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAGTTAGCCCTGTTAGATGGT CCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATA TGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTA CGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATCTTCTGCCTGATGCAGTTT GACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGA AGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCCCAAGTCCAGGGAGTCCCG CGACAAACAGAATGCAACAAGGATGACTAGAATGGGCCAAGAAAAGAAATGGTTTACAGATGAGCCGGAT AATGCCTATCCCAGAAACATTCAAATCAAGCCCATGAGTACCCACATGGCTAACCAGATCAACCAATATA AATCCACAAGCAGCCTGATCCCTCCAATCAGAGAAGTTGAAGATGAATGTTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001253367
Insert Size	3693 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253367.1, NP_001240296.1
RefSeq Size	4970 bp
RefSeq ORF	3693 bp
Locus ID	16531
Gene Summary	Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (11) lacks an in-frame exon and has an alternate splice site in the 3' coding region, which does not affect the reading frame, compared to variant 1. The resulting isoform (11) lacks an internal segment and an internal single aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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