Ttbk2 (NM_001024857) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610507N02Rik; AI326283; B930008N24Rik; mKIAA0847; Ttbk; Ttbk1; TTK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229513 representing NM_001024857 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGT TAAGAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGT GGCGCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAA CTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCA TGCAATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCAC TACCCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGA GACATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATT TTGGCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCG AGGGACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGG TCTTTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGC AAGTAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCAC CTTTCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTT GACAATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATG GCTCCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGG AATTGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAA CAGCTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGC ACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAAT TTGCAAAGCAGCTACTGAAGAAGAAAATAGCCATGGTCAAGTAAATGGCATACTCAATGCTCCAAGCCTT GGTTCACCAATTCGTGTCCGATCAGAGATTACTCAGCCAGACAGAGATGTTCCGTTAGTAAGGAAGCTAC GTTCTATTCACAGCTTTGAGCTGGAAAAACGTTTGACACTTGAGCCAAAGCCAGATACTGACAAGTTTCT GGAGACCTGCATGGAGAAAATGCAGAAAGATTCCAGTGCAGGAAAAGAACCTGTCCCCCCTGCTCTGCCT CATAAGCCGTGTGTCCCCGTCGTGACCCACACTGACCACATCTGGCACTATGATGACGAATATCTTCCTG ATGCCTCCAAGCCTGCCTCTGCCAACACCCCGGAGCAGGCAGATGGTGGTGGCAGCAACGGATTTATAGC TGTTAACTTAAGCTCTTGCAAACAGGAGGTTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAAGAC CTTCAGGACTTTAGGACAAATGAGGTGTTAGGCCATAAGACAACTGGAAGCCCTTCAGATGAGGAGCCTG AAGTGCTTCAGGTCCTTGAGGGATCACCTCAAGATGAAAAGATCCAAGTAGGTCCTTGGACTGACAACCA TCACTTAAAGAAGGAAAGCTCAGGTGTGGTTTTAGCACTTTCTGCTGAATGCCCTGCTACTGCTGCTTCA GAACTGTACACAGATAGGCTAGACCTCCAGGCTGGAGCTGCAAGTCAGTTCATCACAGTGACTCCCACAA GTCCCATGGAGGCACAAGCAGAAGGACCCCTGACTGCGATTACAATTCCTAGACCTTCTGTGGCATCAAC ACAGTCAACTTCAGGAAGCTTTCACTATGGCCCACAACCAGAGAAGAAAGATCTTCAGCCCTTGGAGCCC ACTGTAGAACTGTACTCTCCAAGGGAGAACTTCTCCGGCTTGGTTGTGACAGAGGGTGAACTAGCTAGCG GAGGAAGCAGAGTGGATTTGGGACTTCAGATAGATCACACTGGTCATGACATGTTACCCAACATGAGAGA TGGTGACACATCTCAAGACTTGGGACCAAAAGACCCTCCTGACCATAATAGATTAGCTGTGAAAGAATTT GAACATCTCCCTGGAGAAACAGAAGAGAGAAGCCTTCTTCTGGGCTCAGAGAATGAAGATGAGAGGTTAA GTAAAGGGCAGCACTGTATTGAAGTCTCTTCCCCAGGAGAGTTAGTGACTGCAGAGAGGGCTCAGTTAGC TGCCACAGAACCTCTGCATGTATCAGAGACACAGAACTGTAGTGTGCTACCAAATCAGGACAAAACCCAT GAGATAATGAAGCTTTTGGCAGTTGGAACTTCAGAAATTTCTCCTCAAGCCATTGACCCACATGCTGAAG GGCAGATAGGCCAGATGGCAGCAATGCAGAAAAATAAGCTATTTAAAGATGATGGTATTCAGAGTGAAAG CTTGCCAAGGCAGCAGGGAGACCTCTCTGCTTTTTTGCACCAAGAGGGTAAGAGAGAGAAAGTTGTCCCT AGAAATGGAGAGCTCTATCATTGTGTCTCAGAGAATGAGCATGGTCCTCCTACTCGGAAGGACATGCTCC GGTCATCCTTTGTGACCAGGCACAGCCGGATCCCTGTTTTAGCACAAGAAATAGACTCAACTTTTGAATC ATCCTCTGCTATTTCTGCAAAAGAAAAGCTTCTACAGAAGAAAGCCTATCAGCCAGAAATAGTCAAACTT CTTGTAGAAAAAAGGCAGTTCAAGTCTTTCCTGGGAGACCTCTCAAGTGCCTCTGATAAGCTGATAGAGG AGAAACTAGCCGCTGTTCCAGTCCCTTTTTCTGAGGAGGAAGTCTTCGCTCCCTTTTCTAGACTGGCAGC AGATTCCCACCTGAGTAGATCAGTTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGGCTAGAAAGAGC AAGATTCCAAGGCCAGTATCCTGGGTCAGCACAGATCAAATTAATGGCTCCGCTTCACCTCAGTTCTTGC CTCGGCCACCACCAGGAAAGCCACCAGTCAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTTCT AGGGAGTAGTAACTCTGACTCAGACCTTTTCTCTCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAA TCCCGGAGTACTACCCAATGCAAGAGCCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAG TTGTACCTCGAAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGAACATCTAGTTCCTCACC ATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCTTCCCCACATCTGGGGAGAAGCAAGTCACCC CCTAGCCACTCAGGATCATCGTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATG GCCCAAAAGGATCTGGCAGCCTCCACCACCACTCAACCAGCTCTAAAACTCCCCCAGGGAAGAGTAAGCC AGCCAGTAAACTCAGCAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001024857
Insert Size	3732 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001024857.2, NP_001020028.1
RefSeq Size	11078 bp
RefSeq ORF	3732 bp
Locus ID	140810
UniProt ID	Q3UVR3
Gene Summary	Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) is missing a non-coding exon compared to transcript variant 2. Both variants encode the same isoform (2).

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