Clip1 (NM_001291229) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110007I12Rik; 4631429H07Rik; AV017631; C81039; CLIP-170; Clip50; CLIP170; CYLN1; mKIAA4046
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229555 representing NM_001291229 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATGCTAAAACCCAGCGGCCTGAAGGCCCCTACCAAGATCCTTAAGCCGGGAAGCACAGCCTTGA AGACGCCTGCTGCTGCTGCAGCTCCAGTGGAGAAGACAATACCCAGTGAGAAAGCCTCGGGCCCTCCATC CTCCGAGACCCAAGAGGAGTTTGTGGATGACTTTCGAGTTGGAGAACGTGTTTGGGTGAACGGGAATAAG CCCGGATTTATCCAGTTTCTTGGGGAAACTCAGTTCGCACCAGGCCAGTGGGCTGGGATTGTTTTAGATG AACCCATAGGGAAGAACGATGGCTCTGTGGCGGGAGTGCGGTATTTCCAGTGTGAACCTTTAAAGGGCAT ATTCACCCGACCTTCAAAGCTAACGAGGAAGGTGCAGGCAGAAGATGAAGCCAACGGCCTGCAGGCAGCT CCTGGGAGAACTGCTTCACCTCTGTCCACTGCTGCAGCTACCATGGTGTCTTCTTCTCCAGCCACTCCCT CAAATATTCCCCACAAACCGTCCCAGTCAACGGCAAAAGAACCTTCGGCTACACCTCAAATTAGCAACCT CACAAAAACTGCCAGTGAGTCGATCTCCAACCTTTCAGAGGCTGGCTCTGTCAAGAAGGGGGAGCGGGAG CTCAAGGTCGGAGACAGGGTGCTGGTTGGTGGCACTAAGGCTGGCGTAGTCCGCTTTCTTGGCGAGACCG ACTTTGCCAAGGGGGAGTGGTGTGGTGTGGAGTTAGACGAGCCTTTGGGGAAGAACGATGGTGCTGTGGC CGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGATTGTTCGCTCCTGTCCACAAAGTGACAAAGATT GGCTTCCCGTCTACTACCCCAGCCAAAGCCAAAGCCGCCGCTGTGAGGCGAGTGATGGCCGCCACGCCCG CCAGCCTGAAGCGAAGCCCTTCTGCCTCCTCCCTCAGCTCCATGAGCTCCGTGGCCTCCTCTGTGAGCAG CAAACCCAGCCGGACAGGACTATTGACTGAAACCTCCTCCCGCTACGCCCGCAAGATCTCGGGCACCACT GCCCTCCAGGAGGCGCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCTGAGCGGGACCTGGAGC GGGCCGAGGTGGCCAAGGCTACCAGCCACGTGGGGGAAATAGAGCAGGAGCTAGCCCTGGCCCGAGATGG GCATGACCAGCATGTCCTGGAACTAGAGGCCAAGATGGACCAGCTACGGACCATGGTGGAAGCTGCTGAC AGGGAGAAAGTGGAGCTGCTCAACCAGCTGGAAGAGGAGAAGAGGAAGGTTGAGGACCTTCAGTTCCGAG TTGAAGAAGAATCAATTACCAAAGGCGATCTTGAGGTGGCTACCGTCTCAGAAAAGTCCCGAATAATGGA ACTAGAAAAGGACCTAGCATTGAGAGCACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAGTAAA CCTCCTGGGGATGTGGATATGTCTCTCTCCCTTTTGCAAGAAATAAGTGCTTTGCAAGAAAAGCTAGAAG CCATCCATACTGACCACCAGGGCGAGATGACTTCTTTGAAGGAACACTTCGGAGCTCGAGAGGAGGCGTT TCAGAAGGAGATCAAGGCTCTGCACACGGCCACTGAGAAGCTTTCCAAAGAGAACGAGTCCCTGAGAAGC AAGCTCGACCACGCTAATAAGGAGAACTCCGATGTGATAGCTCTATGGAAGTCCAAACTGGAGACCGCCA TAGCATCCCACCAGCAGGCAATGGAGGAGCTGAAGGTGTCCTTTAGCAAAGGCATTGGAACGGACTCCGC TGAATTTGCTGAGTTAAAGACACAGATAGAGAGACTGAGACTAGATTACCAGCACGAAATAGAAAGTTTA CAGAGTAAACAGGACTCTGAGCGGTCTGCTCATGCTAAAGAGATGGAGACCATGCAGGCCAAGCTGATGA AGATCATTAAAGAGAAGGAGGACAGCCTGGAGGCCGTCAAAGCGAGACTGGACAGTGCAGAAGACCAGCA CCTAGTGGAAATGGAGGACACGCTAAACAAACTGCAGGAGGCGGAGATTAAGGCTAATAGCATTACCAAA GAGCTCCAGGAGAAAGAGCTAGTGCTCACTGGTCTGCAGGACAGTTTGAATCAAGTCAATCAAGTGAAGG AGACTTTGGAGAAAGAACTTCAGACTTTGAAAGAAAAGTTTGCCAGTACTTCAGAGGAGGCGGTCTCTGC TCAGACAAGGATGCAAGATACCGTAAATAAACTGCACCAAAAGGAGGAACAGTTTAACGTGTTGTCGTCT GAACTGGAGAAGTTGAGAGAAAATTTAACAGACATGGAGGCAAAATTTAAAGAGAAGGATGATCGAGAAG ATCAGCTGGTAAAGGCAAAGGAAAAATTAGAAAACGACATTGCAGAAATAATGAAGATGTCAGGAGATAA CTCTTCTCAGCTGACAAAGATGAATGATGAATTACGTCTGAAGGAAAGATCCGTGGAAGAACTCCAGCTC AAGCTTACAAAGGCCAATGAAAACGCCAGTTTTCTGCAGAAGAGTATTGGCGAAGTGACTCTTAAAGCTG AGCAGAGTCAGCAGCAAGCAGCCAGAAAACATGAAGAAGAAAAGAAAGAGTTGGAGGAAAAACTGTTGGA ACTGGAAAAGAAGATGGAAACAAGCTACAACCAGTGTCAGGACCTGAAAGCTAAGTATGAAAAAGCCAGT TCTGAGACTAAAACAAAGCATGAAGAAATCCTGCAGAACCTCCAGAAGATGCTGGCGGACACAGAGGATA AGCTGAAGGCTGCTCAGGAGGCGAACAGAGATCTGATGCAGGACATGGAGGAGCTGAAAACACAGGCCGA CAAAGCCAAAGCTGCTCAGACTGCAGAAGACGCCATGCAGATCATGGAACAGATGACCAAAGAGAAAACA GAAACGCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAGACTACAGAATGAATTGGACACACTTA AGGAAAACAACTTGAAAACTGTGGAAGAGCTGAACAAGTCAAAAGAACTGCTGAGTGTAGAGAACCAAAA AATGGAAGAATTCAAGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCA GCACTGCAGGAAGAGAACGTCAAACTTGCCGAGGAGCTGGGGAGAACCCGGGACGAAGTCACAAGTCACC AGAAGCTGGAAGAAGAACGATCTGTACTCAATAATCAGTTGTTAGAAATGAAAAAGAGAGAATCTGAGTT CAGAAAAGACGCCGATGAAGAGAAAGCCTCCTTGCAGAAGTCGATCAGCCTCACCAGTGCCTTGCTCACA GAGAAGGACGCGGAGCTGGAGAAGCTGAGGAATGAGGTCACAGTGCTCAGGGGAGAGAATGCCACCGCCA AGTCCCTGCACTCAGTTGTGCAGACACTGGAGTCTGATAAGGTGAAGCTTGAGCTCAAGGTCAAAAACTT GGAACTTCAACTCAAGGAGAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAACACTGATGCTCAGGCGGAA GAGGATGAGAGAGCCCAGGAGAGTCAGATTGATTTCCTCAACTCGGTAATAGTGGATCTTCAAAGAAAGA ACCAAGACCTCAAGATGAAGGTGGAGATGATGTCTGAAGCTGCACTCAATGGCAACGGGGAAGACCTGAA CAGTTATGACAGTGATGACCAGGAAAAGCAGTCCAAGAAGAAACCCCGCCTCTTCTGTGACATCTGTGAC TGCTTTGACCTCCACGATACAGAGGACTGTCCCACCCAGGCACAGATGTCGGAAGATCCTCCCCATTCCA CTCACCATGGCAGCCGGAGCGAGGAGCGGCCATACTGTGAGATCTGTGAGATGTTTGGGCACTGGGCAAC CAACTGCAATGACGACGAGACCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291229
Insert Size	3948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291229.1, NP_001278158.1
RefSeq Size	5649 bp
RefSeq ORF	3948 bp
Locus ID	56430
UniProt ID	Q922J3
Gene Summary	Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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