Disp1 (NM_001278219) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1190008H24Rik; DispA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229618 representing NM_001278219 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCTGTGATCAGCGGAAGTGACTCTGTGCTCCTGAGCAATGGCAGCATCTCGACCAGCACCTCCAACC CTAGTCCCCTCAGCCCCAGTGATGGAGACCTCCCAGCCCAGCACCTCGGACCCAGAGAAACCCCAAGAAC AAAAGCAAGCCCAAATGGATGCCTGCAACTTAATGGCACAGTTAAGTCATCCTTTCTGCCTTTAGACAAC CAAAGAACACCTCAGACGCCAACTCAATGCTGCCACCCTTGTCCATACCATCACCCTGTGAGCAGCCATA GCAATCACCAGGAGTGCCATCCCGAGGCTGGCCTTGCAGCCTCCCCCGCTTTGGCCTCGTGTCGCATGCA GCCACATTCCGAATACTCTGCATCTCTGTGTCCAAACCATTCACCCGTGTATCAGGCTGCGCACTGCCTT CAGCCCTCGCCATCTTTCTGCCTTCATCACCCGTGGCCTGACCATTTCCAGCATCAACCTGTACGGCAGC ACCTGACCATCATCAGGCCGTCCAGACCTTTCAAGTTCCCGAGAAGTTACGCAGCCCTGCTGGCTGACTG GCCTGTGGTGGTCCTGGGCATGTGCACGCTGCTCATCGTGGTCTGTGCCCTGGTGGGGGTCCTAGTGCCA GAGCTCCCCGACTTCTCGGACCCGCTGCTGGGTTTTGAACCAAGAGGGACCACGATAGGACAGAGGCTGG TCACGTGGAATAACATGATGCGAAACACAGGGTACAAAGCAACATTGGCCAACTATCCTTATAAGTATGC AGAGGAGCAAGCCCGAAGCCACCGGGATGACCGATGGTCAGACGATCACCATGAGAGAGAGAGAAGAGAA GTGGACTGGAACTTCCAGAAAGACAGCTTCTTCTGCGATGTTCCAAGTGATGGATACTCCAGAGTAGTGT TTGCTTCAGCAGGAGGGGAGACTCTGTGGAATTTACCTGCAATTAAGTCGATGTGTGATGTGGATAATTC CAGGATCAGATCCCACCCCCAGTTCAGCGATCTCTGCCAGAGGACCACTGCCGTCTCCTGCTGCCCGAGC TGGACCCTGGGGAATTACATCGCCATTCTGAATAACAGATCGTCCTGTCAGAAGATTGTTGAGCGAGATG TCTCTCACACGCTGAAGCTGCTCCGGACCTGCGCCAAGCACTACCAAAACGGCACCCTGGGGCCAGACTG CTGGGACAAGGCGGCCAGAAGGAAGGACCAGCTCAAGTGTACCAACGTGCCGCGCAAGTGTACCAAATAC AACGCCGTGTACCAGATTCTCCACTATCTGGTAGACAAGGACTTCATGACCCCAAAGACGGCTGACTACG CCGTGCCAGCTTTAAAGTACAGCATGCTCTTCTCCCCCACGGAGAAAGGGGAGAGCATGATGAACATTTA CCTGGACAACTTCGAAAACTGGAACTCCTCGGACGGCATCACCACCGTCACCGGCATCGAGTTTGGCATC AAACACAGCTTGTTCCAGGATTACCTCCTCATGGACACCGTGTACCCCGCCATCGCCATCGCCATTGTCC TGCTCATCATGTGTGTCTACACCAAGTCCATGTTCATCACGCTCATGACCATGTTTGCCATCATCAGCTC GCTCATTGTCTCCTACTTCCTCTACCGAGTCGTCTTTAACTTCGAGTTTTTCCCCTTTATGAACCTCACG GCGCTCATCATCCTGGTCGGGATTGGCGCGGACGACGCTTTTGTCCTGTGCGACGTCTGGAACTACACCA AGTTCGACAAGCCTCGCGCGGAAACTTCGGAGGCGGTGAGTGTCACCTTGCAGCACGCGGCCCTCTCCAT GTTTGTCACCAGCTTCACCACTGCCGCTGCCTTCTACGCTAACTACGTCAGCAACATCACGGCCATCAGG TGCTTCGGGGTGTACGCGGGGACCGCCATATTGGTGAATTACGTCTTGATGGTCACCTGGCTCCCGGCTG TGATTGTTCTGCACGAGCGGTACCTCCTCAATATATTCACCTGCTTCAGGAAGCCCCAGCCACAAGCCTA CGACAAGAGCTGCTGGGCAGTGCTCTGTCAGAAGTGCCGCAGGGTGCTCTTTGCCGTCTCAGAGGCATCG CGGATTTTCTTTGAAAAGGTGCTGCCGTGCATAGTCATCAAGTTTCGCTACCTTTGGCTCATCTGGTTCC TCGCCCTGACTGTGGGCGGGGCCTACATCGTGTGCGTAAACCCAAAGATGAAACTGCCGTCGCTGGAGCT GTCTGAGTTCCAGGTGTTCCGGTCCTCTCATCCTTTCGAGCGCTATGATGCCGAGTTCAAGAAGCTCTTC ATGTTTGAGCGGGTTCACCACGGAGAGGAGCTGCATATGCCCATCACGGTGATCTGGGGCGTGTCCCCAG AAGACAGCGGCGACCCTCTGAACCCCAAGAGCAAAGGGGAGCTGACACTAGATAGCACGTTCAACATTGC TAGCCCAGCCTCCCAGGCCTGGATTTTACACTTCTGTCAGAAGCTGAGGAATCAGACCTTCTTCCACCAG ACGGAGCAGCAGGACTTCACCAGCTGTTTCATCGAGACCTTCAAACAGTGGATGGAGAACCAGGACTGTG ACGAGCCTGCCCTGTATCCCTGCTGCAGCCACTGCAGCTTCCCCTATAAGCAAGAGGTTTTCGAGCTGTG CATCAAGAAGGCCATCATGGAGCTGGACAGAAGCACGGGATATCATCTGAATAACAAGACCCCCGGGCCA AGGTTCGACATCAACGACACCATCAGGGCGGTGGTGCTGGAGTTCCAGAGCACCTTCCTCTTCACCCTGG CCTACGAGAAGATGCAGCAGTTCTACAAGGAGGTGGACTCGTGGATTTCCCACGAGCTGAGTTCGGCCCC CGAGGGCCTCAGCCGCGGCTGGTTTGTCAGCAACCTGGAGTTCTACGACCTGCAGGACAGCCTTTCCGAC GGCACCCTCATCGCCATGGGGCTCTCGGTGGCCGTTGCCTTCAGCGTGATGCTGCTGACCACGTGGAATA TCATCATAAGCCTGTACGCCATCGTCTCCATAGCTGGAACCATATTTGTCACAGTCGGGTCCCTGGTCCT GCTGGGCTGGGAGCTGAACGTGCTGGAGTCCGTCACCATCTCGGTCGCAGTCGGCCTGTCTGTAGACTTT GCCGTCCACTACGGGGTGGCGTACCGCTTGGCTCCAGATCCCGACCGAGAAGGCAAGGTCATCTTCTCTC TGAGCCGTATGGGCTCTGCGATCGCCATGGCTGCACTGACCACCTTCGTGGCGGGGGCCATGATGATGCC CTCCACGGTCCTGGCTTACACGCAGCTGGGCACGTTCATGATGCTCGTCATGTGCGTGAGCTGGGCCTTC GCCACCTTCTTCTTCCAGTGCCTGTGTCGGTGCCTGGGGCCACAAGGCACCTGCGGCCAGATCCCTTTCC CTACAAAACTCCAGTGCAGTCCCTTTTCCCACACCTTGTCTGCAAGGCCTGGGGACAGGGGACCAAGCAA AACACATGCCGCCAGTGCGTACAGCGTAGATGCCAGGGGCCAGAAATCCCAACTGGAGCATGAGTTTTAC GAGTTACAGCCCCTGGCATCTCACAGCTGCACTTCCTCGGAGAAGACTACGTACGAAGAGCCACACACCT GCTCCGAATTTTTCAACGGCCAAGCAAAGAATTTAAGGATGCCTGTGCCTGCAGCCTACAGCAGCGAACT CACCAAAAGCCCCAGCAGTGAGCCAGGCTCAGCCTTGCTGCAGTCCTGTCTGGAGCAGGACACCGTGTGC CATTTCTCTCTCAATCCGAGATGTAACTGCCGAGACGCCTACACACACTTACAGTACGGATTACCAGAAA TCCACTGCCAGCAGATGGGTGACTCCTTATGCCACAAGTGTGCCTCCACTGCAGGCGGCTTTGTCCAGAT TCAGAGTTCGGTGGCACCTCTGAAGGCCTCACACCAAGCCGCCGAGGGCCTTCTGCACCCTGCCCAGCAC ATGCTGCCCCCAGGGATGCAGAATTCTCGGCCTAGGAATTTCTTTCTCCACTCAGTGCAGCACTTTCAGG CCCAAGAAAACCTGGGTCGCACCAGCACACACAGCACGGATGAGCGTCTTCCCAGGACAGCAGAGCTGTC ACCGCCACCGTCTGACAGCAGGAGCACTGAGTCTTTCCAAAGAGCTTGTTGCCATCCTGAGAATAACCAA AGGAGACTCTGCAAAAGTAGAGACCCAGGGGACACAGAGGGCAGTGGAGGGACAAAATCCAAGGTCTCTG GTTTACCAAACCAGACTGACAAGGAGGAGAAGCAAGTGGAGCCAAGCCTGCTGCAGACCGATGAAACTGT GAACTCAGAACATTTAAATCATAACGAATCAAACTTTACATTCAGCCATTTACCAGGGGAGGCTGGCTGC AGGTCCTGCCCAAACAGTCCACAGAGTTGTAGAAGCATCATGAGATCGAAGTGCGGGACTGAGGACTGCC AGACGCCAAACCTTGAAGCCAATGTGCCTGCTGTACCAACACACTCAGACCTGTCTGGCGAGAGTCTGTT AATAAAAACACTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001278219
Insert Size	4566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278219.1, NP_001265148.1
RefSeq Size	4902 bp
RefSeq ORF	4566 bp
Locus ID	68897
UniProt ID	Q3TDN0
Gene Summary	Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal. Synergizes with SCUBE2 to cause an increase in SHH secretion (PubMed:22902404).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.

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