Rusc2 (NM_001037709) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI840675; mKIAA0375
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229622 representing NM_001037709 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTTGTTGGAACTTTCCAGAATGGATAGCCCCCCTAAGCTGACTGGAGAGACCCTCATCGTCCACC ATATCCCCTTAGTGCACTGCCAAGTCCCAGACAGGCAGTGCTGCGGGGGCGCAGGCGGAGGCGGTGGGGG CACAAGAGCCAATCCCTTCTGCCCCCCGGAGCTGGGCCTCACCCAGCCTGACCACGACCTAGGACAAGCT GACTCCCTTCTGTACCCCAGCCTGCACTCAGCGCCAGGGGCACCTACAGGGTCTTCAGACAGCGTCAAAA GTAGGAGTCGGGATGGACGAGGCCCCGGAGCTCCCAAGCGCCATAATCCCTTCTTGGTGCAGGAAGGTGT GGGTGAGACAGGACTCGGTGACCTGCATGACAGCAGCACTGGTGACAGTGTCACCCAGCAGTCCTTCCAC CTGCACAGCGCCAGCCAGCCCTTCCATCTGTCTTCTTTCCAGCTGCCACCGTCCGGCCCCGGGCAGGGCA GGCCGTGGGGTGCAACTCACAGTCGACCTGGAGTGGTGGAGGGGCAGGAACAGGACCCAGCGACAGCCTT GGGGACCCAGTGCAGCACCAGCCACTGCTGCCGGCCAGAGCTGGAAGCTGAGAGGATGGAGCTGGACGAG TGTGGGGGACACGGGGGGAGTGGCAGTGGAGGGGGGACCAGTGATATCTCTGGCTTTTCCTTTGAGCAAG AGTGGAAGATCAGTTCCGACGAATCCCCAAGACACCCTGGACGCTCCGGCTCGGGAACCCAGCACTGCCA CTGCAGTAGTACGTCCAGTCAGTCGGAGGCGGCTGACCAGTCCATGGGCTATGTCAGCGACTCCTCCTGT AACAGCTCCGACGGTGTGCTAGTCACCTTCAGCACCCTCTACAATAAGATGCACAGCAGCTCCCGTGCCA ACCTCAACTCTGTCCCTCAGTCCTGCAGCGACTCTTCCTTCTGCAGCCACGCAGACCCTGGAGCCTTCTA CCTGGACCTGCAGCCCTCCCCGGCTGAGTCGAGAATGTCTTGCGAGTCCCACCACCCTGAGAACGGGGAC AGGGAAGAGGGCTGTGGCTGTCCTCATGTCTCATCCCCTGAGCTCGATGCCAACTGCAACGCCTACCACC CACACTCTGAGCCCTGCCCAGCTGTGGCTGACCTCACAGCCTGCTTCCAGAGCCAGGCCCGCCTTGTTGT GGCCACACAGAATTACTATAAACTTGTCACCTGTGACCTGTCCTCACAGTCCTCCCCGAGCCCCGCCGGC TCCTCCATCACGAGTTGCTCTGAAGAACACACCAAGATAAGTCCTCCACCAGGCCCCTGCCCAGACCCAG ACCCCAACCAGCCCTCTGAGTATTACCTCTTCCAGAAGCCAGACATCCAGCCAGAGGAACAAGAAGCAGT GGGTTCCCCAGCAGAAGCAGCAACCGCCATGGGCCCCACTGTACTTGAAGGGCAGGTGTACACGAATACT TCCCCCCCCAACCTCAACACTGGCCGGCAGCGCTCTCGAAGCTATGACCGTAGCCTGGAGCGCAGCCCCC CTGTCCGCCTGGGCTCACTAGAGCGCATGCTTAGTTGCCCAGTACGATTAAGTGAGGGCCCTGCGGCCCT AGCTGGGCCTGCGTCCCCACCCAGAAGGGTTACCTCCTTCGCTGAGTTGGCCAAAGGCCGGAAGAAAGCT GCGGGCTCCGGCTCCCCACCACTGCGGGCGAGCGTTGGAGATTCTTCCCAAGAGTTCTCGCCCATCCAAG AAGCCCAGCAAGACAGGGCAGCCCCACTTGACGAGGGCACTCGATGTAGCCATAGCCTGCCGTCCCTGCC CTTGGGGCCAAGCTTGGACCTCCTTGGCCCCGAACCCTGGTCCACCCCGGTTTGTCAGGGCTCCCAGTCC AGCGAGATGCCACTTCCTAGCCTCAGAGCTGCTGGGCAGGGACCCTTGGCCCAGCTGATGGATCCTGGGC CTGCTTTCTCGGGGAGCCCAGCCACCAGCCATACCCAGAGGGATTCGAGAGCTAGAGCTGACGGGGGTGG CACCGAAAGCCGACCCGTCCTTCGCTACAGCAAGGAACAGAGGCCGACCACCCTACCGATCCAGCCGTTC GTGTTCCAACATCACTTCCCCAAGCAGCTGGCCAAGGCCCGGGCCCTGCACAGCCTCTCCCAGCTCTACA GCCTCTCTATGCCCTGCAGCCGCGCACAGCAGCCCGCCCCGCTGGTTATCTCTACTGCTCAAGGCCCAGC CCCAGCTCCCTCAGGAGAGCCGCAGCCATTCACATCGCAGGCCTCTGGCAGAGGCGCCAGAAACGCTGGG CCTGAACCAGAGACCTCGCGGCCATCGCCCCTGGGAAGCTACTCCCCAGTCCGGAGTGCCGGCCCCTTTG GGTCTAGCACCGACTCCTCTGCCTCTACCTCCTGCTCCCCGCCTCCAGAGCAGGGCACAGCAGCCGACAG TGTATCACCATGGAGCCATACCTGCCCTCCTACTGTGCGCCCTGCCACATCCCAGCAGCCACCGAAGGAG GACCAGAAGATTCCGACCTTGGCTGAGTACAGGCTTCATGGCACAGGAAGTTTACCCCCTCTGGGCTCCT GGAGATCTGGCTTCACCCGGGCAGAGAGTCTGGTTCGAGGAGGTGGCGAGGGCAGCATGGCCAACAGGCC CAACAATGCCAACCACCTGTCCCCTCAGGCCCTCAAGTGGCGGGAATACAGGAGGAAGAACCCATTAGGG CCACCTGGTTTGTCAGGAAGCCTAGATCGAAGGCCACCGGAAGCTCGGCTGGCCCGCAGGAACCCCATCT TTGAGTTCCCTGGTTCTTTCGGCACAACCAGCCATCTGAATTGCCGGCTGAATGGTCAGATTTCAAAGCC ACTGTCGCTGACCTGTCCTGACCTGCAGGACCCCTTCTCCTTAACCGAGAAGCCTCCCGCTGAGTTCTGT CTGTCTCCCGATGGCAACTCGGAGGCCATATCCATCGACATACTTCAGAAAAAAGGGCTGGTAAAAGCTG TCAACACCGCTGTGGACCTTATTGTGGCCCATTTTGGCACGAGCCGGGATCCTGGCGTGAAGGCAAAGCT GGGCAACAGTTCTGTGAGCCCCAATGTCGGCCACCTGGTTCTGAAGTACTTGTGCCCTGCAGTCCAGGCC GTGCTCGAGGACGGACTCAAGGCCTTCGTACTGGATGTGATCATCGGCCAACGTAAAAACATGCCTTGGA GTGTGGTCGAGGCTTCCACACAGCTAGGCCCATCCACCAAGGTCTTGCATGGCCTCTACAACAAAGTCAG CCAGTTCCCAGAACTCACCAGTCACACCATGCGCTTCAACGCCTTCATCCTCGGCCTGCTCAACATCCGG TCTCTGGAGTTCTGGTTTAATCACCTGTATAACCATGAAGATATCATCCAGACCCACTACCAGCCCTGGG GCTTCCTGCGGGCCGCACACACGGTGTGCCCAGGCCTCTTCGAGGAGCTGCTGCTGTTGCTACAGCCCTT AGCCCTGCTACCCTTCAGCCTGGACTTGCTGTTCCAGCACCGGCTGCTGCAGAGCGGGCGGCAGCAGCGG CAGCACAAGGAACTGCTGCGTGTGTCCCAGGACCTGCTGCTGTCTGCGCACTCAACACTGCAGTTGGCCA GGTCCCGAGGCCAGGAGGGCCCTGGAGACATGGACAGGGTGGCTCCTGGGGAACGGGTGAAGGGGGTGGG TGCCTCAGAAGGTGGAGAAGAGGACGAGGAAGACGCGGAAGAGGTGGCAGTGGTGGCGGGGAGCTCGGAT CATGGCAAGTGGGCCCGAGGGGGTCAGGCTGGCTGGTGGTACCAGCTCATGCAGAGCTCCCAGGTCTACA TCGATGGCACCGCTGAGGGCTCTAGATTCCCTCGCAGCAGCAGCAGCAGCAGTGGCAGCGGCAGTGAGAA GAAGAAAGGAGTAGGCAGTGGGGGGCCGTCCCAGGCTCCGCCTCCGCCGCCTCGGGAAGGTGTGGTAGAG GGGGCGGAGGCCTGCCCTGCCCCTGAGGAGGCCCTTGGCCAAGAAAGGGGCTGGCCTTTCTGGATGGGGA GCCCCCCTGACTCTGTGCTAGCTGAGCTGAGGCGTAGTCGAGAGAGGGAAGGGCCTGTTGCCCCACCGAC GGAAAATGAGGAAGGGACCGCAGAGCCTTCACCTGGGGGCATCAAGTGGGGACATCTCTTTGGTTCTCGG AAATCTCAGCGGGAAGCCCGGCCCACGAACAGGCTGCCGTCGGACTGGCTGAGCCTGGACAAGTCTGTGT TCCAGCTGGTAGCACAGACGATGGGGGCCCGTCGGGAGCCCGAGCCCAGGGAGAACCTGCAAGAGTCACA CCCTCCAGCCGTGCCCTCCAAACCTCCGTGCGAGGTGCAGGCTCTGTGCCACCATCTGGCCACAGGCCCT GGACAACTGAGCTTCCACAAAGGAGATATCCTCCGGGTGCTGGGACCGGCCAGAGGAGACTGGCTGCGCT GCAGCCGAGGCCCTGACACCGGCTTGGTACCTCTGGCCTATGTGACATTGACCCCAACTCCAAGTTCACC TCCTGGGAGCAGCCAAAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001037709
Insert Size	4572 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001037709.2, NP_001032798.1
RefSeq Size	5317 bp
RefSeq ORF	4572 bp
Locus ID	100213

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