Dnmt1 (NM_001199431) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cxxc9; Dnmt; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD; MommeD2; MTa; MTase
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229641 representing NM_001199431 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG GACAGTGACACCCTTTCAGTTGAAACTTCACCTAGTTCCGTGGCTACGAGGAGAACCACCAGGCAGACCA CCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGAAGAGTCGGAAGAGGGGAACTC GGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTTGTAGACACAGAGAGTGGTGCT GCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGGGTCCGGAAGAGCCATGTGAAC AGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATTGAGGCGGAAATCAAAGGAGGA TCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGACGGAAAAAAGGATAAAAGAAGT TCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCAAGGAAGCAGAGCCAGAGCAGG TAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAAGAGACGAAAAACGACACGTAA AAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAAGCCGCTCAAAGCAAAAGTGTG ATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAGACGACCCTAACCTGAAGTACC AGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGAGAAACTGTCCATCTACGACTC CACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTCACTTCCTTCAGTGTGTACTGC AGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATGTAGAGCTCTACTTTTCTGGGT GTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAATGGCAAAAACCTCGGGCCAAT CAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATTGGCTTCTCCACTGCATTTGCT GAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGCTGATGCAGGAGAAAATTTACA TCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATATGAAGACCTGATCAATAAGAT TGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAGGACTCCCTCTTACGCCACGCC CAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATGATGAGACCCCCATCTTCTTGT CTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACAGAGGCGAGCAACAAGGCGCGT CATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACCACCAAGCTGGTCTATCAGATC TTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACAAGGAGAATGCCATGAAGCGCC GCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGATATGGT GAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCGGTGAAG GAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCATCACCCAAAAAGCTGCATCAGG GGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCCTATGAAGATTGAAGAGAATAG AACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGCGACTGCGTCTCGGTCATTCCA GATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGGAAGACAAAAATGGTCAGATGA TGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGCCACCTCCGACCCCCTGGAACT GTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAAA GCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCACACTGCCTGGGGCTGAGGATG GCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTTTGAATCCCCACCCAAGACCCA GCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAA ATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACTGCAGTTCCATCACCAAGAATG GTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTTTACTTTCAACATCAAAGTGGC TAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTACCCTGAGCACTACCGCAAGTAT TCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCATCGGTCGGATAAAAGAGATCC ACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAGGCTCTACAAGTTCTACAGGCC TGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAACATGCTTTACTGGAGCGACGAG GAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGTACGGGGAAGACCTACTTGAGA GCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGAGGCCTACAATTCAAAGACCAA GAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGGAAAGGGAAGGGGAAAGGGAAG GGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCATCAAACTGCCCAAGCTCCGGA CCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCAAGCAGGCATCTCGGAAACGCT GTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAACAACCCCGGCACCACAGTGTTC ACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGACCAACTCTCTGGGCCAAAGGC TGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCAGGGCTTCAGTGGCATGAACCG CTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCCTTCCTCAGCTACTGTGACTAC TACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCTACAGACGCTCCATGGTGCTGA AGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGGTGTGCTCCAGGCTGGACAGTA TGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCAGGAGAAAAGCTGCCTCTGTTC CCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTGTGGTGGATGACAAGAAGTTTG TTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGTGCGAGACACCATGTCTGACCT CCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGAGAGCCACTGTCCTGGTTCCAG AGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCCCACTGG TGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCGTGACCTGCCCAACATACAGGT GCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCATGATGTGAAAAATGGCTACAGC AGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCTGCGACCCTGAGTCCAGGCAAT TCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTACGG GCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAGCCCATGGGCAAGCAGGGTCGG GTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCCGCTCCCAGGGCTTTCCAGATA GCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAATGCTGTGCCACCACCCCTGGC CAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGGGAGAGCGCATCAGCTGCAGTT AAAGCAAAAGAGGAGGCTGCTACCAAGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199431
Insert Size	4863 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199431.1, NP_001186360.2
RefSeq Size	5370 bp
RefSeq ORF	4863 bp
Locus ID	13433
UniProt ID	P13864
Gene Summary	This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) encodes the longest protein (isoform 1). This variant has two upstream in-frame AUGs but N-terminal sequencing and western analysis from PMID: 9830015 (Table I and Figure 2) support the use of the third in-frame AUG.

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