Wnk3 (NM_001271679) Mouse Untagged Clone

CAT#: MC229655

Wnk3 (untagged) - Mouse WNK lysine deficient protein kinase 3 (Wnk3), transcript variant 2



  "NM_001271679" in other vectors (1)

CNY 16,530.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Prkwnk3; Wnk3-ps
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229655 representing NM_001271679
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTGAAA
ACAGGGCTGCCCGGGCTGTTGCCCCTCTGACTGTAGAAGCGAGAATAAAGGAAAAATACAGTACCTTTTC
TGCTTCCGGGGAAAACATCGAAAGAAAGAGGTTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA
GTTGCCGAATCTTCCCGCAGAGATGAAAGAAAAGCTGCAACAAATATTTCAAGAGTAGATAAAGTTCCTA
CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAATGTTCAAAGGCAACCTCAGAATCCTCAAA
AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTTGCAACTTCC
CCTAGTGGCAGGTTCCTGAAATTCGATATAGAGCTGGGAAGAGGAGCATTTAAAACGGTATATAAAGGAT
TGGACACTGAGACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA
GCAACGGTTCAAAGAAGAAGCAGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTCTATGAT
TCATGGGAGTCCACTTTGAAAGGAAAGAAATGTATTGTGTTAGTGACTGAACTAATGACATCAGGAACCT
TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATCTT
AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT
TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCGT
TTGCTAAGAGTGTCATTGGCACTCCTGAATTTATGGCTCCGGAGATGTATGAAGAACACTATGATGAATC
TGTAGATGTTTATGCTTTTGGAATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC
CAGAATGCAGCACAAATATACCGGAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG
ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCTATCAAGGA
CTTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC
TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA
ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA
GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGAGTGACATTGATT
AAAAAGATAAGGGAAAAAAAGCCTGCTGGATGCTTGGAGGAACGCAGGGATTCCCAGTGCAAGTATGTGA
GGAATGTACTTCCTCAACAGCAGACTGCAACTTTACAACCTACTCCTGGTCCACACACTGCAGCTGAGTA
TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGTTTCTACAGGGCAAACCACAGCAGCAGTCCTCC
TCTGTTAGGGGTGACACTTCATCTGAGCCAACAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCATC
CCACTGTAGCCTATTCTTCAAACCAGACAACTAGCTCTCAGGAGCAGCCAAAGCTGACTCAGTCCCCAGT
TTTGCCTGTGGTTCAAGGTCAGTCCTCTGTTATGCCCATATATGCTGCTGGAGTTGGAGTTGTTTCACAG
TCCCAAATCTCTCCCTTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG
AACAACAAGCAACTCTTCAAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA
AAACACCAATAACCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGAACAAACAACGAAGAGCCTCC
TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGAGACAACA
TGGTGGAGTGTCAGCTGGAAACACATAACAACAAGATGGTGACATTCAAGTTTGATGTTGATGGTGACGC
ACCTGAGGATATCGCTGACTATATGGTTGAAGATAACTTTGTGCTGGAAAATGAGAAAGAAAAATTTGTC
GAGGAATTGAGGGCTATCGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTCAGCTGTAGAAAAATCTA
TTGGTGTTGACTCTGTTGCCTTGGAATCTAACAGTAACCAAACAGGATCATCTGAACAAGTACTGATAAA
TTCTGCATCCACTCAAACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGT
ATCAACCAAACAATAAAAAACCGTGAGGCACAGTCTCCTCCTTCCCTTCAGCCTTCCATGGCCATGGTGC
CTGGGTTACATCCATTTCCTAGTTCAAGAAACACAAGTAATCAGGCAATATCACAGAACACAGTGTTCAC
TATAGAGAATAATCCAGGCCATCGTGAACTTTTCACCTCCAAATTAGACCACAAGGATGTGGTTGATGGT
AAGATTGGTGAACATGCTAGTATAGAAACTGAGCAGTCAAGTATTTCCTATCAAGTGGAAGATGACAGAC
AGATCATGACACCAGCTACTGATAATTCCAATTATTCTGCTGCTTTGGTGTGTCCAGTTCCAGGTGAATG
TGAGGCACTCACCAGCCAAGCAGGCATGTTCATGCCTACATATCCAAATCAGCAAGCTGCTGTTCTGGCT
GATGTGCATATAGCCTATCCCGGTGAATCAGTTCCAATTGGTGGTAATGCTGCTCTTACATCAGTACTTG
TATCTTCTGATCAAAAGCCTCAATCCTTGTCAGTACAGCAGCCAACTATAGATGCAGAGTTTATTTCCAA
AGAAGGAGAAACCACAGTAAACACCGAAACAAGTTCTCCTAAGGCAGTCATTGCCACTCAGACTCCTGGC
TTTGAACCAGCTGTGATTCTACCTGCTACTATCCTGGAATCAGATGGGGAACGACCTCCAAAAATGGAGT
TTGCAGACAACCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTATCAGGAGCACAGCATTTC
TAGCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTTTCTTCCTCTGCTGAAGAT
ATACTATCCTATTCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTCAAGATAGCCCTGCACAAT
CCCCAAATTTCCAACAAACAGGCTCTAAGATTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTCATATATC
AGTGTTCAAAAAAGACCTGAATGTGATAACTTCTGTACCCAGCGAGTTATGTTTACATGAGATGTCCCCA
GATGCTTCACTTCCAGGCGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGATGGAACCATTCATCTGC
AGACAGGAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCATTCCTCTGACACGGGAGTT
CTCATCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCAG
GTGATTACTGTTCCTCAGCAGCAGCCAGTAAAAATGATGTCTTTTGGGAAAGATCACAGACCGCCATTCA
ACAAAACAACAGTCCAGTCTAGTGAGCAAGCACTAACCTTTGCTGAAGCTGCAGTGTCGCAGTTGATCGA
AGTGGAGCCTGCCATGCCCACTCATAAAGCTTCAGTTTCTTCACGCAAGTTGCGAACTCTTTATGAAACC
TTTAAAGAAGATAAAGGAGATCCTGAACAAGGTGACATCGTATCTTACAGCACAGCTTGTGAGACCAGTG
TCTCTTCAGTCGCTACAGAAAAGAATGTAACTTCAACCACAGAAGTTAGTGTGCAGTCTGGGTCTGAACC
GTTAGATAAAGAAAAAAATGAGTCAACTCCTGGGAAACAGACATGTACAAATGAATTTTCAGCCACTCTT
GCTGGTAATAGAAAGTCCGTGACAAAAACTCGTCCAGAGGGTGATCAGTATTTACCACTCCGTGAAGAGC
AAGCCTATGCTCAAACACAGAATTCACTCTTCTATTCTCCATCTTCCCCAATGAGCAGTGACAATGAATC
AGAAATTGAGGACGAGGACTTGAAAGTGGAGCTTCAAAGATTACGAGAAAAACACATTCAAGAGGTGGTC
AGTCTTCAAACACAACAGAATAAAGAGCTACAAGAGCTCTATGAACGCCTTCGAGCAACTAAAGACAACA
AAGCGCAATCTTCAGAGGTTCCTTTGTCACCTGCATCGCCACGCAGGCCAAGATCTTTCAAAAGCAAACT
TCGAAGCCGCCCCCAGTCCATGACACATTCAGACAATCTCGTTGTAAAAGATGCACTGGGTGTGGAGAGT
AATACCGTGTCATGCCAGCAATCTCCAGCCAGTAAGAAGGGGATGTTCACAGATGATTTACACAAGCTGG
TGGATGACTGGACAAGAGAAACAGTAGGGCATTTTCCTAGTAAGCCAAGTTTAAACCAACTCAAACAGAG
CCAACAAAAATCCGAAGCAGAAAACTGGAACAAGTCATGTGAAAGTACTCCATCTACTATGGGATACACG
TCAAACTGGATTTCTTCTCTCTCCCAAATTCGTGGAGCTGCCCCAACTTCCTTGCCACAAGGACTCCCAC
TCCCTTCATTTCATGGGCCATTAGCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGGTGC
CGCAGGTTATCCAGTACAGTGGGTAGGAATTTCTGGACCAGCACAACAGTCTGTAGTGCTTCCTACCCAA
TCTGGGGGACTCTTCCAGCCCGGGATGAATTTGCAGTCATTTCCAGCTCCACCAGTGCAGAATCCAGCTT
CAATCCCTCCTGGTCCTAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001271679
Insert Size 5133 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271679.1, NP_001258608.1
RefSeq Size 10259 bp
RefSeq ORF 5133 bp
Locus ID 279561
UniProt ID Q80XP9
Gene Summary Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has an alternated splice site in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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