Tsc2 (NM_001286716) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Nafld; Tcs2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229659 representing NM_001286716 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCAACAAGCAAAGATTCCGGCTTGAAGGAGAAGTTCAAGATACTGTTGGGATTGGGAACAT CGAGGCCAAATCCCAGGTGTGCAGAAGGCAAACAGACTGAGTTTATCATCACATCGGAAATCTTGAGAGA ACTGAGTGGTGAATGCGGCCTCAACAATCGCATCCGAATGATAGGGCAGATCTGTGATGTGGCAAAAACT AAGAAGCTTGAGGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC CACCAGAGGCCCGGCATGCAGTTCTCACCTTATTGAAGGCCATTGTACAGGGACAGGGTGATCGTTTGGG AGTTCTCAGAGCCCTCTTCTTCAAAGTGATCAAGGACTACCCCTCCAATGAAGACCTCCATGAAAGGCTA GAAGTTTTTAAGGCCCTCACAGACAATGGGAGGCACATTACCTATTTGGAAGAAGAACTGGCAGAGTTTG TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTTGTCAACCTGGTCAAGTTCAA CAGCTGTTACCTTGACGAATACATTGCATCAATGGTTCACATGATTTGTCTGCTATGCATCCGGACAGTG TCCTCTGTGGACATTGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCTTACCAGCCG AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCATCAATGTCAAGGAGCTGTGTGAGCCTTGCTG GAAGTTGATGCGTAACCTTCTGGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCGCATCATG GAGGACAGATCCTACATGGAAGATGCCCCACTGCTGAGAGGAGCTGTGTTCTTTGTAGGGATGGCACTCT GGGGAGCTCACCGGCTCTACTCTCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCTAT GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTATAGG AAGGAGCTCCAGGCTGTGACATGGGATATTCTGCTGGACATCATTGAACGACTACTTCAGCAACTCCAGA ACCTGGACAGCCCGGAACTCAAGACCATCGTCCATGACCTGCTGACCACTGTGGAGGAGCTATGTGACCA GAACGAGTTCCATGGCTCGCAGGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGACCTGAA TCCTCTCTCTTAAACTTGATATCCTACAGAGCCCAGTCCATCCACCCTGCCAAGGATGGCTGGATCCAGA ACTTGCAGTTGTTGATGGAGAGGTTCTTCAGGAATGAGTGCCGCAGCGCCGTGCGCATCAAGGTGTTGGA TGTTCTGTCCTTCGTGCTGCTGATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCGGTGGTCATC TCGCAGCTCTCCCACATTCCCGAGGATAAGGACCATCAGGTCCGAAAGCTGGCTACTCAGCTGCTGGTGG ACCTGGCAGAGGGGTGCCACACCCACCACTTCAACAGTCTGCTGGACATCATTGAAAAGGTGATGGCGCG CTCACTCTCTCCACCCCCGGAGCTGGAAGAAAGGGACCTGGCCATGCACTCGGCCTCCCTGGAGGACGTG AAGACCGCGGTCCTGGGGCTCCTGGTCATCCTTCAGACCAAGCTCTACACCTTGCCTGCCAGCCACGCCA CTCGAGTGTATGAGAGCCTCATTAGTCACATCCAGCTCCATTACAAGCACGGCTACTCCCTGCCCATTGC TAGCAGCATCCGACTACAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC CTGCCCAACAAGGATGGGGTCGTGAGATTCAGCCCTTACTGCCTCTGTGACTGCATGGAACTGGATAGAG CCTCAGAGAAGAAAGCCAGTGGGCCCCTTTCACCTCCAACTGGGCCCCCTAGCCCTGTGCCTATGGGCCC TGCTGTGCGGCTCGGCTACCTACCCTACTCCCTGCTCTTCCGTGTCCTGTTGCAGTGTTTGAAGCAGGAG AGCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAGGCTGCCGGAGTCACTGCGCTACAAAGTCCTCATCT TCACCTCCCCCTGCAGTGTCGACCAGCTGTCTTCTGCCCTCTGCTCCATGCTTTCAGCTCCAAAGACCCT TGAGCGGCTCCGAGGTACCCCAGAAGGCTTCTCGAGAACTGACCTGCACTTGGCTGTGGTTCCCGTGCTA ACAGCATTAATATCTTATCACAACTATTTGGACAAGACCAGACAGCGTGAGATGGTGTACTGCCTGGAAC AAGGCCTTATCTACCGCTGCGCCAGCCAGTGCGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA CATCATCATCAAGGCGCTTCCTGTCCTGGTGGTGAAGCTCACACACATTTCTGCCACAGCCAGCATGGCC ATCCCGCTCCTGGAGTTCCTGTCTACTTTGGCCAGGCTGCCCCACCTCTACAGGAACTTTGCTGCAGAGC AGTATGCCAGCGTGTTTGCCATCTCCTTGCCATACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG TCTGGCTCACCACGTCATAGCCATGTGGTTCATCAGGTGCCGACTGCCCTTTCGGAAGGATTTCGTCCCT TATATCACTAAGGGTTTGCGTTCCAATGTCCTCCTGTCTTTTGATGATACCCCTGAGAAGGACAGTTTCA GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGCAGAATACAGACATCCCTTACGAGCGCCAGCCT GGGGTCTGCGGATGAGAACTCTATGGCCCAGGCTGATGACAACTTGAAGAATCTCCACTTGGAGCTCACA GAAACATGTCTGGACATGATGGCCCGATATGTGTTCTCCAATTTCACTGCAGTCCCCAAGAGGTCCCCTG TGGGAGAGTTCCTCCTGGCAGGGGGTAGGACCAAAACCTGGCTGGTTGGAAACAAGCTTGTCACTGTGAC AACAAGTGTGGGAACTGGTACACGGTCGCTGCTGGGCCTGGACTCTGGGGACCTGCAGGGTGGCTCGGAT TCAAGCTCTGATCCTAGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTG GGCAGCAGGTGTCCCGTGGGGCCCGGGACCGGGTCCGCTCCATGTCAGGGGGCCATGGCCTTCGAGTTGG TGTCCTGGATACTTCAGCTCCCTATTCCCCAGGTGGCTCTGCTTCTCTGGGACCACAGACGGCGGTGGCA GCGAAGCCCGAGAAGCCCCCTGCAGGAGCCCAGCTTCCAACAGCAGAGAAGACGAATCTGGCAGCCTATG TGCCTTTGTTAACCCAGGGCTGGGCAGAAATCTTAGTCCGCAGACCCACAGGAAACACCAGCTGGCTGAT GAGCTTGGAGAACCCGCTCAGCCCCTTCTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCCAAT GCCCTTATGGCTGCTGAACGTTTCAAGGAGCACCGGGACACGGCCCTGTACAAGTCATTGTCAGTGCCAG CGGCTGGCACCGCCAAGCCTCCTACTCTCCCACGCTCTAATACAGACTCAGCCATGGTCCTTGAGGAGGG AAGTCCAGGAGAGACTCAGGTGCCAGTGGAGCCCCCCGAATTGGAGGATTTCGAGGCGGCGTTAGGTACA GACAGGCACTGCCAGCGGCCTGACACCTACAGCCGGTCATCCTCAGCATCTAGCCAGGAAGAAAAGTCCC ATTTGGAGGAGCTTGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTGC CGTGGACCTCTCCTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCGGAGTTGCAGACCTTA CAGGACATCCTTGGAGACCTAGGGGACAAGATTGATATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGGT CCCAGTCAGGGATCCTGGATGGGGAAGCAGCTACCTGGTCAGCTACGGGTGAAGAGAGCCGTATCACAGT CCCACCTGAAGGTCCTCTGCCTTCCAGTTCTCCCCGCTCCCCCAGTGGCCTCCGGCCCCGAGGCTATACC ATCTCTGATTCCGCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGATAACTTCAAGAGCAGAGCTGCGG CCTCCAGTGCTGAGAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCGCCCTT TTTTGGTGATGAGTCCAATAAGCCCATCCTATTGCCCAATGAGTCCTTTGAACGGTCAGTACAGCTGCTT GACCAGATTCCATCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTG AGCTGGCCATCCTGTCAAATGAGCATGGCTCTTACAGGTACACGGAGTTTCTGACAGGCCTGGGTCGGCT TATTGAGCTCAAGGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTATGTGGCGAGGATGGA CAGTTCACCTACTGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCCACCCTGATGCCCACCA AGGATGTGGACAAGCACCGCTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAA TGATTCTGGTGAGGACTTCAAACTGGGCACCATTAAGGGCCAGTTCAACTTTGTCCATGTGATCATCACA CCGCTGGACTACAAGTGCAACCTATTGACCCTGCAGTGCAGGAAAGACATGGAAGGCCTCGTGGACACCA GTGTGGCCAAGATTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATAT GGCCTCACAGGTACACCACAGCCGATCCAACCCCACTGACATCTATCCCTCCAAGTGGATCGCAAGACTC CGCCACATTAAGCGTCTCCGCCAGAGGATCCGTGAAGAGGTGCACTATTCCAACCCAAGCTTGCCTCTGA TGCACCCTCCAGCCCACACCAAAGCCCCAGCTCAAGCCCCTGAGGCTACACCCACCTATGAAACAGGCCA GCGGAAGCGCCTCATTTCCTCCGTGGATGACTTCACAGAGTTTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286716
Insert Size	5229 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286716.1, NP_001273645.1
RefSeq Size	6132 bp
RefSeq ORF	5229 bp
Locus ID	22084

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