Scn5a (NM_001253860) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mH1; Nav1.5; Nav1.5c; SkM1; SkM2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229693 representing NM_001253860 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA TCGAGAAGCGCATGGCTGAAAAACAAGCCCGTGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCTGCC AGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGCAAC CCACCCCGAGAGCTCATTGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCTTCA TCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTACGTCCTCAGCCCCTT CCACCCGGTGCGCAGAGCGGCTGTGAAGATTTTGGTACATTCGCTCTTCAGCATGCTCATCATGTGCACC ATCCTAACCAACTGTGTGTTCATGGCACAACACGACCCTCCGCCTTGGACCAAATATGTTGAGTACACCT TCACCGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCGTTCAC CTTCCTCCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCATACACAACTGAATTTGTG GACCTGGGCAATGTCTCAGCCTTACGCACCTTCCGAGTCCTCCGGGCCCTGAAAACTATATCGGTCATTT CAGGCCTGAAGACAATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAGCTAGCCGATGTGATGGTCCTTAC TGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAGTGC GTGCGTAACTTTACTGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGATGGCATAGTCTGGAACTCTC TGGACGTCTACCTCAATGACCCAGCCAATTACCTACTCAAGAATGGCACCACGGATGTGTTGCTGTGTGG GAACAGCTCTGATGCCGGGACATGTCCTGAAGGCTACCGGTGCCTGAAGGCAGGTGAGAACCCTGACCAC GGTTACACCAGCTTCGACTCCTTCGCCTGGGCTTTCCTTGCGCTCTTCCGCCTGATGACACAGGACTGCT GGGAACGTCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTTGTCAT ATTCCTGGGCTCCTTCTACCTTGTGAACTTGATCCTGGCTGTGGTGGCCATGGCTTACGAGGAGCAAAAC CAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGTTTCCAGGAGGCCATGGAGATGCTCAAGAAGG AACACGAGGCTCTCACCATCAGGGGCGTGGATACTGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTTGGC CCCAGTAACCAACCATGAGAGAAGGAGCAAGAGGAGGAAACGACTGTCTTCAGGGACAGAGGATGGCGGA GATGACAGGCTCCCCAAGTCGGACTCAGAAGACGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCCATG GGCTCAGCCGGACATCCATGAGGCCCCGCTCCAGCCGAGGGAGCATTTTCACATTCCGGCGACGGGACCA AGGCTCCGAGGCAGATTTTGCAGATGACGAGAACAGTACAGCAGGAGAGAGCGAGAGCCACCGCACATCG CTGCTGGTGCCCTGGCCCCTGCGCCGTCCCAGCACCCAAGGGCAGCCCGGCTTTGGAACCTCAGCTCCTG GTCATGTCCTCAATGGCAAAAGAAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGCAGG GGACGCAGAGGCCACCTCTCCAGGGAGCCACCTTCTCCGCCCTATAGTGCTGGACAGACCCCCAGACACG ACCACGCCGTCAGAGGAGCCGGGTGGGCCCCAGATGCTGACACCGCAGGCTCCATGTGCAGATGGCTTTG AGGAGCCCGGGGCACGGCAACGGGCACTCAGCGCCGTCAGTGTCCTCACCAGCGCTCTGGAAGAGTTGGA GGAGTCCCACCGTAAGTGTCCACCATGCTGGAATCGCTTTGCCCAGCATTACCTCATCTGGGAGTGCTGC CCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTCGTGGTCATGGACCCGTTTGCTGACCTCACCATCA CCATGTGCATCGTGCTCAACACGCTCTTCATGGCTCTGGAGCACTACAACATGACAGCCGAGTTTGAGGA GATGCTGCAGGTCGGAAACTTGGTCTTCACGGGAATCTTCACGGCAGAGATGACCTTCAAGATCATCGCC CTTGACCCATACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGCCTCA TGGAGCTGGGCCTGTCTCGCATGGGCAACCTGTCTGTGCTGCGTTCCTTCCGTCTGCTGCGGGTCTTCAA GCTGGCCAAATCATGGCCCACCCTGAACACACTCATCAAGATCATCGGGAACTCTGTTGGTGCCCTGGGG AACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGTATGCAGCTCTTCGGCAAGA ACTACTCAGAGCTGAGGCACCGAATCAGCGACTCTGGCCTGCTGCCCCGCTGGCACATGATGGACTTCTT CCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGAGAGTGGATCGAGACCATGTGGGACTGCATGGAG GTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTGGTCATGGTCATTGGCAACCTTGTGGTCCTGA ATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGGGGA GATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAAAGGGGCCTTCGCTTTGTCAAGCGGACCACCTGG GACTTCTGCTGCGGGCTCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGCTGC CCAGCTGTATCGCCGCTCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGCGCCCCCAGCCCGCAAGGA AACACGGTTCGAGGAAGACAAGCGGCCGGGCCAGGGCACCCCTGGGGATACCGAGCCTGTGTGTGTTCCC ATCGCAGTGGCTGAGTCAGACACTGATGACCAGGAAGAGGATGAGGAGAACAGCCTTGGCACGGAGGAGG AAGAGTCCAGCAAACAGCAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCCCCCAGGAGCCCAGGGC CTGGAGCCAGGTGTCAGAGACCACATCCTCTGAAGCCGAGGCCAGTACGTCTCAGGCAGACTGGCAGCAA GAGCGGGAAGCGGAGCCCCGGGCCCCGGGGTGCGGTGAGACTCCCGAGGACAGTTACTCCGAGGGTAGCA CCGCTGACATGACCAACACCGCAGACCTCCTGGAGCAAATCCCAGACCTCGGGGAGGACGTCAAGGACCC AGAGGACTGCTTCACTGAAGGCTGCGTCCGACGTTGTCCTTGCTGCATGGTGGACACGACGCAGGCCCCA GGGAAGGTCTGGTGGAGACTGCGCAAGACCTGCTACCGCATAGTGGAGCACAGCTGGTTCGAGACTTTCA TCATCTTCATGATCCTGCTCAGCAGCGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGAC CATCAAGGTTCTGCTGGAGTACGCCGACAAGATGTTCACCTATGTCTTTGTGTTGGAGATGCTGCTCAAG TGGGTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTAATTGTGGACG TCTCACTGGTCAGCCTCGTGGCCAACACCTTAGGCTTTGCCGAGATGGGTCCCATCAAGTCCCTGAGGAC ACTGCGTGCACTTCGACCCCTGAGGGCTTTGTCACGATTCGAGGGCATGAGGGTGGTGGTCAACGCGCTG GTGGGCGCCATCCCCTCCATCATGAATGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCA TGGGCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACCGAAGGGGACCTGCCTCTGAA CTACACCATTGTGAACAACAAGAGCGAGTGTGAGTCCTTCAACGTGACTGGAGAGTTGTACTGGACCAAG GTGAAAGTCAACTTTGACAACGTGGGGGCTGGGTACCTGGCCCTGCTGCAGGTGGCGACATTTAAAGGCT GGATGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAAGAGCAGCCGCAGTGGGAGGACAACCT CTACATGTACATCTACTTCGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTGTTTATTGGT GTCATCATTGATAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGACATCTTCATGACGGAGGAGC AGAAGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTCT GAACAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTTACCATCATGTTCCTC ATCTGTTTAAACATGGTGACCATGATGGTAGAGACAGATGATCAGAGCCCTGAAAAAGTCAACATCTTGG CCAAGATCAACCTGCTCTTCGTGGCCATCTTCACAGGAGAGTGTATTGTCAAGATGGCTGCCCTGCGCCA CTATTACTTCACCAACAGCTGGAACATCTTCGATTTCGTGGTTGTCATCCTCTCCATCGTTGGCACTGTC CTCTCCGACATCATCCAGAAGTACTTCTTCTCCCCAACGCTCTTCCGTGTCATCCGTCTGGCCAGGATCG GCCGCATCCTCAGGCTGATCCGAGGAGCCAAGGGGATTCGCACGTTGCTCTTCGCCCTCATGATGTCCCT GCCCGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCC AACTTTGCCTACGTCAAGTGGGAGGCTGGCATCGACGACATGTTCAACTTCCAGACCTTTGCCAACAGCA TGCTGTGCCTTTTCCAGATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGG ACCCCCCTACTGTGACCCCAACCTGCCCAACAGCAATGGCTCCAGGGGGAACTGTGGGAGCCCAGCAGTG GGCATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCCATCA TCCTGGAGAACTTCAGTGTGGCCACAGAAGAGAGCACAGAGCCCCTGAGCGAGGATGACTTCGACATGTT CTATGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATTTGGCCCTGTCCGACTTT GCCGATGCCCTGTCTGAGCCACTCCGCATCGCCAAGCCCAACCAGATAAGCCTCATCAATATGGACCTGC CCATGGTGAGCGGGGACCGCATCCACTGTATGGACATCCTGTTCGCCTTCACCAAGAGGGTGCTCGGGGA GTCTGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAATTCATGGCGGCCAATCCTTCCAAGATC TCCTATGAGCCCATCACCACCACCCTGCGGCGAAAACATGAGGAGGTGTCGGCCACGGTCATCCAGCGGG CCTTCCGGAGGCACCTCCTGCAGCGCTCGGTGAAGCATGCTTCCTTCCTCTTCCGCCAGCAAGCGGGCAG CAGTGGCCTCTCTGATGAGGATGCCCCCGAGCGAGAGGGACTCATTGCCTACATGATGAATGAGAACTTC TCCCGGCGCAGCGGTCCCCTCTCCAGCTCTTCCATCTCCTCCACGTCCTTCCCACCGTCCTATGACAGCG TCACGAGGGCCACCAGTGATAACCTCCCAGTGCGTGCGTCGGACTACAGCCGCAGCGAAGATCTTGCAGA CTTCCCTCCATCTCCAGATAGGGACCGAGAGTCTATAGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253860
Insert Size	6063 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253860.1, NP_001240789.1
RefSeq Size	8287 bp
RefSeq ORF	6063 bp
Locus ID	20271
UniProt ID	Q9JJV9
Gene Summary	This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:11834499, PubMed:23420830). It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) switches an exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is the same length as isoform 1 yet it has a different sequence. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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