Myof (NM_001302140) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310004N10Rik; 2310051D19Rik; E030042N20Rik; Fer1; Fer1l3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229700 representing NM_001302140 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGAGTGATTGTGGAATCTGCCACTAACATCCCTAAAACAAAATTTGGGAAGCCGGATCCTATCG TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAACTCAACCCTGTCTGGAA TGAGATTTTGGAATTTGACTTGAGGGGGATACCACTGGACTCTTCATCCTCCCTGGTGATTGTTGTCAAA GATTTTGAGACAATTGGACAAAATAAATTAATTGGCACAGCAACTGTCTCCCTGAAGGACCTGATTGGGG ACCAGAACAGGTCTTTGCCCTACAAACAGACCTCCCTGCTAAATGAAAAGGGACAAGACACTGGGGCCAC CATTGACCTGGTGATCGGCTATACCCCACCTTCAGCTCCACATCCAAATGATCCGAGCGGGACCAGTGTG CCAGGCATGGGAGAAGAAGAGGAAGAAGATCAAGGAGATGAAGACAGGGTAGATGGCATAGTCAGAGGCC CTGGGCCCAAGGGGCCATCTGGGACAGTGTCAGAAGCTCAGCTGGCTCGGAGGATCACCAAAGGGAAGAG CAGCCGAAGGATGCTGTCCAACAAGCCACAGGATTTCCAGATCCGTGTCAGAGTGATCGAGGGCCGTCAG TTATGCGGCAACAACATAAGGCCCGTGGTGAAAGTTCACATCTGTGGTCAGACGCACCGCACGAGAATCA AGAGAGGGAACAATCCGTTTTTTGACGAGTTATTTTTCTACAATGTGCACATCACACCCTCTGAGCTCAT GGATGAGATCATCAGTATCCGGGTTTATAATTCCCATTCTTTGAGGGCAGATTGTCTGATGGGGGAATTT AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCACGCTGTCATGAGGAAATGGCTCCTTCTCAATG ACCCAGAAGACACCAGCTCAGGCGCAAAAGGTTACATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA CGAACCCCCTCCTGAGAAACGAGACCGTGACAATGACAGTGATGACGTGGAGAGCAACTTGTTACTTCCG GCAGGCATTGCCCTCCGGTGGGTGACCTTCATGCTGAAGATCTACCGAGCCGAGGACATCCCCCAGATGG ATGATGCCTTCTCACAAACAGTGAAGGAAATATTTGGTGGCAACGCAGATAAAAAAAATCTTGTGGATCC TTTTGTAGAAGTCTCCTTTGCTGGGAAAAAGGTGTGCACAAATATAATTGAGCGGAATGCAAACCCTGAA TGGAATCAAGTTGTCAACCTTCAGATCAAGTTTCCCTCCATGTGTGAAAAAATAAAACTAACAGTATATG ACTGGGACCGGCTTACTAAAAATGATGTAGTTGGGACCACGTACCTGTACCTCTCTAAAATTGCTGCCTC TGGGGGAGAAGTGGAAGATTTCTCATCCTCGGGAGCTGGGGCTGCATCATATACAGCAACTACAGGAGAG ACAGAGGTGGGGTTTGTCCCAACATTCGGACCTTGTTATTTGAACCTTTACGGAAGCCCCAGAGAGTACA CAGGATTTCCAGATCCCTATGATGAGCTCAACTCAGGAAAGGGAGAAGGGGTGGCCTACAGAGGCAGGAT CTTCGTGGAACTGAACACGTTCCTTGAAAAGAAACCACCAGAAAAGAAGCTGGAGCCCATTTCCAGTGAT GACCTTCTGGTTGTTGAGAAGTACCAGCGAAGGCGGAAGTATAGCCTGTCCGCCGTGTTCCATTCAGCCA CCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAGTCAGCATTGGGAACTATGGCAACAAGTTTGA TGCAACCTGTAAACCTTTGGCATCAACCACTCAATACAGCCGTGCAGTATTTGACGGGAACTACTATTAC TACTTACCTTGGGCCCACACGAAGCCCGTGGTCACCCTGACTTCCTACTGGGAGGATATTAGTCACCGCC TGGATGCAGTGAACACTCTCCTAGTCATGGCAGAACGGCTGCAATCAAACATAGAGGCTGTAAAATCAGG GATCCAGGGTAAAATTCCTGCAAACCAGTTGGCGGAGGTGTGGCTGAAGCTGATAGATGAAGTTATAGAA GACACGAGATACACCTTGCCTGTCACAGAAGGAAAAGCCAACGTCACGGTTCTGGATACTCAGATCCGAA AGTTGCGGTCCAGGTTTCTCTCCCAGATACACGAGGCAGCTCTGAGGATGAGATCTGAAGCCACGGATGT GAAATCCACCTTGTTGGAAATCGAGGAGTGGCTTGATAAACTGATGCAGCTTACTGAAGAGCCTCAGAAC AGCATGCCTGACATCATCATCTGGATGATCCGTGGAGAGAAAAGACTGGCTTATGCACGAATCCCTGCCC ACCAAGTCTTGTACTCCACCAGCGGTGGGAACGCCTCTGGAAAATACTGTGGGAAAACCCAAACCATCCT TCTGAAGTATCCACAAGAAAAAACCAATGGGCCCAAAGTGCCTGTGGAGCTGCGCGTGAACATCTGGCTG GGTTTAAGTGCTGTGGAAAAGAAGTTCAATAGCTTCGCGGAAGGCACCTTCACCGTCTTTGCTGAAATGT ATGAAAACCAAGCCCTTGTGTTTGGGAAGTGGGGCACCTCTGGACTGGTGGGACGCCATAAATTCTCTGA TGTCACAGGAAAAATAAAACTCAAGAGAGAATTCTTTTTACCTCCCAAAGGCTGGGAGTGGGAAGGAGAC TGGGTGGTTGACCCTGAACGAAGCCTCCTGACTGAGGCTGACGCAGGTCACACCGAGTTCACTGATGAAG TCTACCAGAATGAGAATCGCTACCCCGGAGGCGAGTGGAAGCAGGCAGAGGACACCTATACGGATGCGAA TGGAGATAAGGCGGCATCACCCAGTGAGATGACCTGCCCTCCAGGTTGGGAGTGGGAGGATGACGCATGG ATATATGACATAAATCGAGCAGTGGATGAGAAAGGCTGGGAGTATGGGATTACCATTCCTCCTGATAATA AGCCCAAATCCTGGGTTGCAGCAGAAAAGATGTATCACACTCACAGGCGGCGGAGACTGGTCCGGAAACG CAAGAAAGATCTGACACAGACGGCTTCTAGTACCGCAAGGGCCATGGAAGAACTTGAAGACCGGGAGGGC TGGGAATATGCTTCGTTAATCGGCTGGAAATTCCACTGGAAACAGCGCAGTTCAGACACCTTTCGCCGCA GGCGCTGGAGGAGGAAAATGGCCCCTTCTGAGACACACGGTGCCGCTGCCATCTTTAAACTCGAAGGTGC CCTTGGGGCAGACACCACAGAGGATGGAGAAGAGAAGGGCCCGGAGAAGCAGAAGCACAGCGCCACCACT GTGTTTGGAGCAAACACGCCCATTGTTTCCTGTAATTTCGACAGAGTCTACATCTACCACCTGCGCTGCT ACATTTATCAAGCCAGGAACCTCATGGCTTTGGACAAAGACAGCTTTTCAGATCCATACGCCCATGTCTC CTTCCTCCATCGAAGCAAAACCACTGAGATCATCCACTCCACCCTGAATCCCACCTGGGACCAAACCATT ATATTCGATGAGGTTGAAATCTTTGGGGAACCCCAAACAGTTCTACAGAACCCACCCAACGTTACCATAG AACTTTTTGACAATGACCAAGTGGGCAAAGATGAATTTTTAGGTCGAAGCATTTGCTCTCCCTTGGTGAA ACTGAACTCAGAAACGGACATCACACCTAAGCTTCTCTGGCATCCTGTTATGAATGGAGACAAGGCCTGT GGGGATGTCCTCGTAACAGCAGAGTTGATTCTGAGGAACAAGGATGGCTCCAACCTTCCCATCCTCCCCT CTCAAAGAGCACCAAACTTATACATGGTCCCTCAGGGGATCAGGCCTGTGGTTCAGCTCACTGCCATTGA GATCCTAGCTTGGGGCTTAAGAAATATGAAAAACTACCAGATGGCTTCTGTCACATCACCCAGCCTGGTG GTGGAATGCGGAGGAGAGAGAGTAGAATCGGTGGTGATCAAAAGCCTTAAGAAGACACCCAACTTCCCAA GTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCAAGGAAGAATTGTACATGCCTCCGCTGGTGATCAAGGT CATTGATCACAGGCAGTTTGGGCGGAAGCCTGTGGTTGGCCAATGCACCATTGACCACCTGGACCGCTTT CGGTGTGATCCATATGCAGGAAAGGAGGATATTGTACCACAACTAAAAGCCTCTCTGATGTCTGCACCTC CATGCCGGGAAGTCGTTATTGAAATAGAAGACACCAAACCATTACTGGCTTCTAAGCTGTCGGAAAAGGA GGAAGAAATCGTTGACTGGTGGAGTAAATTTTATGCTTCCTCTGGAGAGCATGAAAAATGTGGACAGTAT ATTCAGAAAGGCTATTCCAAACTCAAGATCTATGACTGTGAACTGGAGGATGTAGCCGACTTTGAAGGCC TGACAGATTTCTCAGATACCTTCAAGCTGTACAGAGGCAAGTCCGATGAGAATGAAGATCCATCTGTGGT AGGAGAGTTTAAAGGCTCCTTCCGAATCTACCCCTTGCCGGATGACCCCAGTGTGCCAGCCCCTCCCAGG CAGTTTCGGGAACTGCCTGACAGTGTCCCACAGGAATGCACTGTTAGGATTTACATTGTTCAAGGCTTAC AGCTCCAGCCTCAGGACAACAATGGCCTGTGTGATCCTTACATAAAAATAACCTTGGGCAAGAAAGTAAT TGAAGACCGAGACCACTACATTCCCAACACTCTCAACCCAGTGTTTGGCAGAATGTACGAACTGAGCTGC TACTTGCCTCAAGAGAAAGACCTGAAGATCTCTGTCTATGACTACGATACCTTCACCCGAGATGAGAAGG TGGGAGAGACAACCATTGACCTGGAGAACCGCTTCCTTTCCCGGTTCGGGTCTCACTGCGGAATCCCTGA GCAGTACTGTGTTTCCGGAGTAAATACCTGGCGAGATCAGCTGAGACCAACGCAGTTGCTTCAAAACGTT GCAAGATTCAAAGGCTTCCCGCCACCAGTTCTTTCTGAAGACGGAAGCAGAATCCGATACGGAGGTCGAG ACTACCATTTGGATGAGTTTGAGGCCAACAAAATCCTGCACCAGCACCTTGGAGCCCCCGAAGAGCGTCT TGCCCTCCATATTCTCAGGACTCAGGGGCTGGTCCCTGAGCACGTGGAGACAAGGACTTTGCACAGCACC TTCCAGCCCAATATTTCCCAGGGGAAACTTCAGATGTGGGTGGATGTCTTCCCCAAGAGTTTAGGACCAC CAGGCCCCCCTTTCAACATCACACCCAGGAAAGCCAAGAAGTACTACCTGCGTGTGATCATCTGGAATAC CAAGGACGTTATCTTGGATGAGAAGAGCATCACTGGGGAGGACATGAGTGACATCTACGTCAAAGGCTGG ATTTCCGGCAGCGAAGAAAACAAACAGAAAACAGATGTCCACTACAGGTCCTTGGATGGTGAAGGCAATT TTAACTGGAGATTTGTTTTCCCATTTGACTACCTCCCTGCTGAGCAGCTCTGTATAGTCGCTAAAAAAGA GCATTTCTGGAGTATTGATCAAACAGAATTTCGAGTCCCTCCTCGGCTGATCATTCAGATATGGGACAAT GACAAGTTTTCCCTGGACGACTACTTGGGTTTTCTGGAACTTGACTTACATCGTACGATCATCCCAGCCA AGACATCAGAGAAATGCAGTTTGGACATGATTCCAGACCTCAAAGCCATGGACCCTCTTAAAGCTAAGAC TGCCTCACTCTTTGAACAGAGGTCCATGAAAGGATGGTGGCCGTGCTACGCAGACAAAGATGGCACCCGG GTGATGGCTGGGAAAGTGGAGATGACACTGGAGGTGCTCAACGAGAGGGAGGCCGACGAGAGGCCAGCCG GGAAGGGGCGGAGTGAACCCAACATGAACCCCAAGCTTGACCCACCAAACCGACCGGAAACCTCCTTCCT CTGGTTCACGAACCCTTGCAAGACCATGCGCTTCATCGTGTGGCGCCGGTTCAAGTGGGTGATCATCGGC CTGCTTCTGCTGCTCATCCTGTTGCTGTTCGTGGCTGTGCTCCTGTACTCTCTTCCGAACTATTTATCAA TGAAGATTGTGAGGCCGAATGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302140
Insert Size	6186 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302140.1, NP_001289069.1
RefSeq Size	7133 bp
RefSeq ORF	6186 bp
Locus ID	226101
UniProt ID	Q69ZN7
Gene Summary	The protein encoded by this gene is a member of the ferlin family of proteins, which have been implicated in fusion events in muscle tissue. Members of this family have a carboxy-terminal single pass transmembrane domain and multiple C2 domains, which bind negatively charged phospholipids in the presence of calcium ions. This gene is expressed at high levels in myoblasts and upregulated in damaged skeletal muscle. Mice deficient in this protein display defects in myoblast fusion, muscle regeneration, and angiogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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