Ralgapa1 (NM_001286263) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310003F20Rik; 4930400K19Rik; AI563624; Garnl1; GRIPE; mKIAA0884; Tulip1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229701 representing NM_001286263 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGACCTTAAACAGTTTTT TGACCAACATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG GATAACTGCAGCAAAGAACAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTCTCAGCACCACAATCCG AATATGGACCTCGGACTTTAGATAATCTTATTAATCCTCCACTCAGCCTTCAAGAAACTCAAGTAACTAT AGAGGAAGTCACTCCTCTTGTTCCGCCACAGTCAGGAGATAAAGGACAAGAAGACCTCACAAGCTATTTT CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACATTTG CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGATCAGACCAAAGCCTCATTATGTGATG ATAAAGAAGGATGCTGAAACCAATGAAACAATATATTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTTA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATTCAGGACCTAATATTCCTGGGAT GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTTTCCAGAGAAGAAAGCAAA AATGATACTGTTGATAAAGTAGACAAGTCTGCAGAGCCTGAGCAGTCTCATTCCAACACAAGCACTCTCA CGGAGCGAGAGCCTAGCTCTTCCAGTCTCTGCAGCATCGACGAAGAACATCTCACAGACATCGAAATTGT TCGCAGAGTGTTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTGAAAGTGTATCAAGAATGGATTCAACAAGAGG AAAAGCCTTTGTTTATGCAAGAGCCTGAGGACACTGCGATCACTTGTTCAGATATACCCTGCAGCGAAAC TGTTGCAGACCATGACAGTGCAATAGAAGATGGAGAGAAAAGAGAAGAGGAAAATGGGACCAGTACTTCT GAACATGTTCGCAATTCTAGCTGGACAAAAAATGGTTCCTACCAAGAAGCTTTTCATGTCTGTGAAGAAG CCACAGAACAGAATATTCAAGCTGGCACTCAGGCCGTTTTGCAGGTGTTTATTATAAACTCATCGAACAT ATTTCTTCTTGAACCTGCAAATGAAATAAAGAATCTTCTGGATGAGCATACAGATATGTGTAAACGCATT CTTAATATTTATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTGCTCG TGCTGCTCAGAGTCACGGAGTCCGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA AAGCATGACCCTGGCGGGTCGACTTGCAGGACCACTTTTTCAGACTCTCATTGTTGCCTGGATCAAAGCA AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCGGTATTGTCATCATTGACCTATT GGGAAGAATTGGCCACTGAGTGGTCACTGACCATGGAGACACTGACTAAAGTTTTGGCTAGAAATTTATA TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA GGAGTTGGACACGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC CTGGCCAGGCACCTATGAGACAGAGAAGTGCAACGACCACTGGCTCCCCAGGAACTGAGAAGGCCAGGAG TATAGTACGGCAAAAAACTGTTGCCATGAGAAGCCGATCCATTGGTGAATGTGCTCTGCCATCGGCCTAT ATTCGCAGTGCTAAAAGTGCTCCTGTTCTGATCCATACTTCCAAACCCTTCTTGCCTGATATTGTTCTCA CTCCCCTTTCTGATGAGCTTTCAGATATTGATGATGCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACA TTTTTCACAGAGTGAAGACACTGGAAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGA AGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGA GCCCCAAACTGCCTCCTCTTAATAGTGAGACTGGTGGCAACAGCGCCAATGTTCCTGACCTGATGGATGA GTTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGGAGTAGTCCAGGCAGC CTTGAAATCCCGAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGGCCTGTTGATGACCCTG GTGTACCCTCCGAATGGACGTCTCCAGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTC GGATTCCTTCAGTGCCTTCCAGTGTGAGGGACGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGG ATCCCATCTTCTGCTGATGTGGATTTAGGTTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCCA GCCTAACTACGCTTCATTTAGATTCAGAAACAAGCAGCCTTAATCAGCAAGCCTTCTCTGCAGAAGTTGC AACCGTTACTGGTTCAGAAAGTGCTTCCCCCGTTCATTCAGCTCTGGGATCCAGGTCGCAGACCCCCTCC CCATCTACACTGAGCAGAGCTCACATAGAACAGAAGGACCTACAGCTCGATGAGAAGCTCCACCACTCTG TTCTTCAGACGCCGGATGACCTAGAAATCAGTGAATTTCCATCTGAATGTTGTAGCGTGATGGCAGGAGG TACTCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGCAGAATGCTGGGCATTTTGGGAGAT GTCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATC TAGCCAAGATCAGAGATAACCTTGGCATTTCAGCTGACAACCTGACTTCCCCTTCTCCACCCGTCCTGAT TCCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGT AAATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCTCCAAATAGAGATT TTTTAACACATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATTGTCAATACGAT CATCAAGCACTGCTCACCTCAGTTTTTTTCACTCGGTTTGCCTGGGGCCACGATGCTTATTATGGATTTT ATTATAGCAGCTGGGAGAGTGGCTTCTTCGGCTTTTCTTAATGCACCAAGAGTGGAAGCACAAGTTCTTC TGGGATCTTTAGTTTGCTTTCCCAACTTATATTGTGAATTGCCTGCTCTCCATCCCAACATTCCTGATAT TGCTGTGTCTCAATTTACAGATGTTAAGGAACTTATAATTAAAACTGTATTAAGCTCGGCAAGAGATGAG CCCTCTGGTCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATTTGGATTTGTGAAGAATTAGTCCACG AATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAAC AGTTGCCCACGTCGCCTGTAACATGCTTCACATGCTGGTTCACTATGTCCCTAGACTTCAGATTCACCAG CCGCAGTCTCCCCTGAAAATTATTCAGATCCTAATAGCAACTATCACCCACCTTTTGCCAAGTACAGAAG CTTCATCATATGAAATGGACAAGAGGTTGGTTGTCTCATTACTTCTTTGCCTTCTGGACTGGATCATGGC CTTACCTTTAAAGACACTGCTCCAACCAGTCCATGCTACAGGAGCAGAAAATGATAAGACCGAAAAGTCT GTTCTCAACTGCATTTATAAGGTATTACATGGGTGTGTCTATGGAGCGCAAAGTTTTAGCAATCCCAAGT ACTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGATCCGTTTATGCATTTGGAAAGTCTGAA AGAACCTGAACCTTTACACTCTCCAGATTCAGAGCGGTCTTCTAAACTGCAGCCTGTGACAGAAGTGAAA ACTCAGATGCAACAAGGATTAATCTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACTTGG GCCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCATGACAATCATTA CAGTGAAAGTACTGAACTTTCTCCTGAGCTCTTTGAAAGTCCAAACATCCAGTTCTTTGTGCTGAATAAT ACAACCTTAGTGTCCTGTATCCAGATAAGATCGGAAGAGAGTATGCCTGGAGGAGGTCTAGCTGCTGGCC TTGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGC CATACTGTATGGACCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTGAC CAAGAGAAACCGGAAGAGCCTCCTACATCTAACGAATGCTTAGAAGACATCGCAGTAAAAGATGGGCTTT CTCTCCAGCTTAGAAGATTTAGAGAAACTGTACCAACTTGGTCTACGATACGAGAGGAAGAAGATGTTCT TGACGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAGTGTTTACAGAGAACGGGAATCTCCCTCAAT GTTCCTGCTCCACAGCCTCTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGC AATACACGGAAGAAAAAGAGTTTGTAGAGAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGA TGAGCCAACACCTCAGAAACCTCAGTCAGCTTTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATG AATTCCTGGGACAAACGGAGGAGCTTTCATCTCTTGAAGAAAAATGAAAAGCTGCTCAGAGAACTTAGGA ACTTGGATTCAAGGCAATGCCGAGAGACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGA TAAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTAGCTGGCCTTGGTTGG GAGGTAAACCTTACAAACCACTGTGGCTTCATGGGAGGCCTTCAGAAGAACAGAAGCACTGGACTGACCA CTCCCTATTTTGCTACCTCGACAGTAGAAGTAATATTTCATGTATCTACACGAATGCCTTCTGATTCTGA TGACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTGCACATTGTTTGGTCAGAACATACT AGAGACTACAGGAGAGGAATTATTCCTACTGAGTTTGGTGATGTCCTCATTGTAATATACCCAATGAAAA ACCACATGTTCAGTATCCAGATCATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCACTTTTTGATGGTGC TATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAA TCACTGATTCCATTGTATCAAAATTTCTATGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACC ATTTAGAGCCAACAACATTTGAAGACTTTGCAGCACAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCC TGCTGACGCAGATCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286263
Insert Size	6249 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286263.1, NP_001273192.1
RefSeq Size	8421 bp
RefSeq ORF	6249 bp
Locus ID	56784
UniProt ID	Q6GYP7
Gene Summary	Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB (By similarity). May interact with the HLH region of TCF3/isoform E12.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) encodes isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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