Cad (NM_001289522) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410008J01Rik; AU018859; Cpad
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229707 representing NM_001289522 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCTGGTGTTGGAGGACGGGTCGGTGCTGCAGGGCCGGCCCTTTGGGGCCGCTGTGTCGACTG CTGGGGAAGTGGTGTTTCAGACCGGCATGGTCGGCTACCCAGAGGCCCTCACTGACCCTTCCTACAAAGC TCAGATCCTAGTGCTAACGTATCCTCTCATCGGCAACTACGGCATTCCCTCAGATGAAGAGGACGAGTTC GGCCTGAGCAAGTGGTTTGAATCCTCGGAGATCCACGTGGCGGGACTTGTAGTGGGAGAATGCTGTCCCA CACCCAGCCACTGGAGTGCCAACTGCACCCTGCATGAGTGGCTGCAACAGCGTGGCATCCCCGGCCTGCA AGGAGTGGACACTCGGGAGCTGACTAAGAAGTTGCGGGAGCAAGGGTCTCTTTTGGGGAAGCTGGTCCAG AAAGGGACAGAGCCGTCAGCCCTGCCATTCGTGGACCCCAATGCCAGGCCCCTGGCACCAGAGGTCTCTA TTAAGACTCCACGGGTATTCAATGCAGGGGGTGCCCCTCGGATCTGTGCTCTGGATTGTGGCCTCAAGTA TAATCAGATCCGATGTCTCTGCCAGCTTGGGGCTGAAGTTACCGTGGTACCCTGGGACCACGAGTTAGAC AGTCAGAAATATGACGGTCTCTTCCTAAGTAATGGACCCGGTGACCCTGCCTCTTACCCGGGTGTGGTGT CCACACTGAGCCGAGTCTTGTCTGAACCTAACCCCCGACCTGTGTTTGGAATCTGCCTTGGACACCAGCT GTTGGCTTTAGCCATCGGGGCCAAAACTTACAAGATGAGATACGGAAACCGAGGCCACAACCAGCCCTGT TTACTGGTGGGCACTGGGCGCTGCTTTCTGACGTCCCAGAATCATGGGTTTGCAGTGGACGCAGATTCGC TGCCAGCAGGCTGGGCTCCTCTGTTCACCAATGCCAATGACTGTTCCAATGAAGGCATAGTGCACGACAG CCTGCCCTTCTTCAGTGTCCAGTTTCACCCAGAGCACCGGGCCGGCCCTTCAGATATGGAACTGCTCTTC GATGTATTTCTAGAAACTGTGAGAGAGGCTGCAGCTGGGAACATTGGGGGCCAGACAGTAAGAGAACGCT TGGCTCAGCGCCTCTGTCCCCCTGAGCTCCCCATTCCTGGTTCTGGGCTCCCGCCTCCACGAAAGGTTCT AATCCTAGGCTCAGGGGGCCTCTCCATTGGCCAAGCTGGAGAATTTGACTACTCGGGCTCTCAGGCAATT AAAGCCCTAAAGGAGGAAAACATCCAGACATTACTAATCAACCCCAACATTGCCACAGTGCAGACTTCTC AGGGGCTGGCAGACAAGGTCTATTTCCTTCCCATAACACTTCACTACGTAACCCAGGTAATTCGTAATGA ACGCCCAGATGGTGTGTTACTGACTTTTGGGGGTCAAACAGCCCTGAACTGCGGTGTGGAGCTGACCAAA GCTGGGGTACTGGCTCGATATGGGGTCCGGGTCTTGGGTACACCCGTGGAGACCATTGAACTGACTGAGG ACCGACGAGCCTTCGCTGCCAGGATGGCTGAGATCGGAGAGCATGTCGCCCCCAGTGAAGCGGCAAATTC TCTTGAACAGGCTCAGGCAGCTGCCGAGCGACTAGGCTACCCTGTGCTGGTGCGAGCAGCCTTTGCCCTG GGTGGTCTCGGTTCTGGCTTTGCTTCCACCAAAGAGGAACTCTCAGCTCTTGTGGCTCCAGCTTTTGCCC ATACCAGTCAGGTGCTGATAGACAAGTCTCTGAAGGGCTGGAAGGAGATTGAATATGAGGTGGTGAGAGA CGCCTATGGCAACTGCGTAACGGTATGCAACATGGAGAACTTAGACCCACTGGGCATTCATACTGGTGAA TCCATAGTGGTGGCTCCGAGCCAGACACTGAATGACAGAGAGTACCAGCTTCTGCGACGGACAGCTATCA AGGTCACTCAGCACCTGGGCATTGTCGGGGAGTGCAATGTACAGTATGCTTTGAACCCCGAGTCTGAGCA GTATTACATCATTGAAGTGAATGCCAGGCTCTCCCGAAGCTCTGCCCTGGCCAGTAAGGCCACAGGCTAT CCACTAGCCTATGTGGCAGCCAAGCTAGCATTGGGCATTCCCCTGCCAGAGCTCAGAAACTCTGTTACGG GAGGAACAGCAGCCTTTGAGCCCAGCCTGGACTACTGCGTGGTAAAGATTCCTCGCTGGGACCTCAGCAA GTTCCTACGTGTCAGTACGAAGATTGGGAGTTGCATGAAGAGTGTTGGCGAGGTCATGGGCATCGGGCGG TCCTTTGAGGAGGCCTTCCAAAAGGCCCTGCGCATGGTCGATGAGAACTGCGTAGGCTTCGACCATACAG TAAAGCCAGTCAGCGACATGGAGCTGGAGACTCCGACGGATAAGCGGATCTTTGTGGTGGCTGCCGCTCT GTGGGCTGGCTACTCTGTGGAGCGCCTCTACGAGCTCACCCGCATTGATTGCTGGTTCCTGCACCGGATG AAGCGCATTGTGACCCACGCCCAGTTGCTGGAACAACACCGTGGACAAGCTTTACCCCAAGACCTGCTGC ACCAGGCCAAGTGCCTCGGCTTCTCAGACAAGCAGATTGCCCTGGCAGTCCTGAGCACAGAGCTGGCTGT TCGGAAGCTGCGTCAGGAACTGGGGATCTGCCCAGCGGTGAAACAGATTGACACAGTTGCAGCTGAGTGG CCAGCCCAGACAAATTACCTGTACCTGACATACTGGGGCAACACCCATGACCTCGACTTTCGGGCACCTC ACGTCCTGGTCCTTGGCTCTGGTGTCTACCGGATTGGCTCCAGTGTTGAGTTTGATTGGTGTGCTGTGGG TTGCATCCAGCAGCTCCGGAAGATGGGTTATAAGACCATCATGGTGAACTACAACCCAGAGACAGTCAGC ACCGACTATGATATGTGTGACCGACTCTACTTTGATGAGATCTCTTTTGAGGTGGTGATGGACATCTATG AGTTAGAGAACCCCGAAGGTGTGATCCTGTCCATGGGTGGGCAGCTGCCCAACAACATGGCCATGGCTTT GCATCGGCAGCAGTGCCGAGTGCTGGGCACCTCCCCCGAAGCCATCGACTCAGCCGAGAACCGATTCAAG TTCTCCCGGCTCCTAGATACCATCGGTATCAGCCAGCCTCAGTGGCGAGAGCTCAGTGACCTTGAGTCTG CTCGCCAGTTCTGCCACACGGTCGGGTACCCCTGCGTAGTGCGCCCCTCCTATGTGCTCAGCGGTGCTGC TATGAATGTGGCGTACACCGACGGGGACCTGGAGCGTTTCCTGAGCAGTGCGGCCGCCGTCTCTAAGGAG CACCCCGTAGTCATCTCCAAGTTCATTCAGGAAGCAAAGGAGATCGACGTGGACGCGGTGGCCTGTGACG GCATCGTGTCGGCCATTGCCATCTCTGAGCACGTGGAGAATGCAGGTGTGCATTCAGGTGATGCCACGCT GGTGACCCCCCCACAAGACATCACCCCGAAAACTCTGGAGCGGATCAAAGCCATTGTGCATGCTGTGGGC CAGGAGCTACAGGTTACAGGACCCTTCAATCTGCAGCTCATTGCCAAGGATGACCAGCTGAAGGTTATTG AATGCAACGTGCGTGTATCTCGCTCTTTCCCCTTCGTGTCCAAGACACTGGGTGTTGATCTAGTAGCCTT GGCCACAAGGATCATCATGGGGGAGAAAGTAGAGCCCGTGGGGCTCATGACTGGCTCTGGAGTCGTGGGA GTAAAGGTACCTCAGTTCTCCTTCTCACGCTTGGCCGGTGCCGACGTGGTACTTGGTGTGGAGATGACCA GTACTGGAGAGGTGGCCGGCTTCGGAGAGAGCCGTTGTGAAGCCTACCTCAAAGCCATGCTCAGCACTGG CTTTAAGATCCCAGAGAAGAACATCCTGCTGACTATTGGCAGCTACAAGAACAAAAGTGAGCTGCTCCCA ACTGTGCGGTTGCTGGAGAGCCTGGGCTACAGCCTCTATGCCAGCCTGGGTACGGCAGACTTCTACACTG AGCACGGGGTCAAGGTAACAGCTGTGGACTGGCACTTTGAAGAGGCGGTGGATGGTGAGTGCCCACCACA ACGGAGCATCTTGGATCAGCTGGCTGAAAATCACTTTGAGCTAGTGATTAACCTGTCCATGCGTGGGGCT GGGGGTCGGCGTCTTTCCTCCTTCGTTACCAAGGGCTACCGTACCCGGCGTCTGGCCGCTGATTTCTCTG TGCCTCTCATCATTGACATCAAGTGCACCAAACTCTTTGTGGAGGCCCTAGGCCAGATCGGCCCAGCCCC TCCTTTGAAAGTGCACGTTGACTGCATGACCTCCCAGAAGCTAGTGCGCCTGCCCGGATTGATTGATGTC CACGTGCACCTTCGGGAACCAGGTGGGACACACAAAGAGGACTTTGCCTCAGGCACAGCTGCTGCCTTGG CTGGGGGTGTCACCATGGTTTGTGCTATGCCTAATACCCGACCCCCCATCATTGATGCTCCTGCTCTCGC CCTTGCCCAGAAGCTGGCAGAGGCTGGCGCCCGCTGTGACTTTACCTTATTCCTTGGAGCCTCATCTGAG AACGCGGGGACTCTGGGTGCTGTGGCCGGCTCTGCAGCAGGCCTGAAGCTCTACCTCAACGAGACCTTCT CTGAGCTGCGGCTGGACAGTGTGGCCCAGTGGATGGAGCATTTTGAAACCTGGCCTGCCCACCTCCCCAT TGTGGCCCATGCAGAGCGGCAGAGTGTCGCTGCGGTCCTCATGGTGGCTCAGCTGACCCAGCGTCCAGTG CACATATGTCATGTGGCTCGGAAGGAAGAGATCCTGTTGATTAAAACTGCAAAGGCGCAGGGGCTGCCAG TGACCTGTGAGGTCGCTCCTCACCACCTGTTCCTAAACCGGGAAGACTTGGAGCGCCTGGGACCTGGGAA GGGAGAGGTCCGTCCTGAGCTTGGGTCCCGAGAGGATATGGAAGCCCTGTGGGAGAATATGGCAGTCATC GACTGTTTTGCCTCAGACCACGCTCCCCATACCTTGGAAGAGAAGTGTGGGCCCAAGCCTCCACCTGGCT TCCCGGGCCTAGAGACCATGTTGCCGCTGCTGCTGACAGCTGTGAGCGAGGGCCGCCTCAGTCTGGATGA CCTGCTGCAGCGATTGCACCACAACCCTCGGCGGATCTTCCACCTGCCTCTCCAGGAGGACACCTATGTA GAGGTGGATCTGGAGCATGAGTGGACAGTCCCCAGCCACATGCCCTTCTCTAAGGCCCGCTGGACCCCAT TTGAAGGACAGAAGGTGAAGGGCACAGTGCGCCGTGTGGTCTTGCGAGGGGAGGTCGCCTATATTGATGG ACAGGTGCTGGTACCCCCGGGCTATGGACAAGATGTACGGAAGTGGCCGCAGGGGGTTGTTCCCCAGCCC CCTCCTTCAACTCCCGCCACCACCGAAATCACCACGACACCTGAGCGACCACGCCGAGTCATCCCGGGCC TTCCTGATGGCCGCTTCCACCTGCCACCCCGCATCCACCGGGCCTCTGACCCAGGTCTGCCAGCTGAGGA ACCTAAAGAGAAGCCACCCAGGAAGGTAGTGGAACCAGAGCTGATGGGAACCCCTGATGGTCCCTGCTAC CCTGCACCACCAGTACCTAGACAGGCATCACCCCAGAACCTAGGGTCTTCTGGCCTGCTGCACCCCCAGA TGTCGCCCCTACTGCACTCCCTAGTGGGCCAGCACATCCTGTCTGTCAAGCAGTTCACTAAGGATCAGAT GTCTCATCTGTTCAATGTGGCGCACACGCTACGGATGATGGTACAGAAGGAGCGGAGCCTCGACATCCTG AAGCTGGCAGCCAAGCACTGTCGCAGGCCAGTGATCAATGCTGGGGATGGAGTTGGGGAGCACCCCACAC AGGCCCTGCTGGACATCTTCACCATTCGAGAAGAGCTGGGGACTGTCAATGGCATGACGATCACTATGGT CGGTGACCTGAAGCATGGACGCACAGTGCACTCCCTGGCCTGCCTGCTCACCCAGTACCGTGTGAGCCTA CGCTACGTGGCACCTCCCAGCCTGCGCATGCCACCCAGTGTACGGGACTTTGTGGCCTCCCGAGGCACCA AGCAGGAGGAGTTTGAGAGCATCGAGGAGGCGCTGCCCGACACAGACGTGCTCTACATGACTCGGATCCA GAAAGAACGATTTGGCTCTGTCCAGGAATATGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCTCACATC ATGACCCGGGCCAAGAAGAAGATGGTGGTGATGCACCCAATGCCCCGTGTCAATGAGATAAGTGTGGAGG TGGACTCGGACCCCCGGGCAGCCTACTTCCGCCAAGCTGAGAACGGCATGTACATCCGCATGGCCCTGCT TGCCACCGTGCTTGGCCGTTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289522
Insert Size	6465 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289522.1, NP_001276451.1
RefSeq Size	6924 bp
RefSeq ORF	6465 bp
Locus ID	69719
UniProt ID	B2RQC6
Gene Summary	This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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