Flnb (NM_001081427) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL024016; Fln-b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229754 representing NM_001081427 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGACGGAGAAAGACCTGGCGGAGGACGCGCCGTGGAAGAAGATTCAGCAGAACACGTTCACGC GCTGGTGCAACGAGCACCTCAAGTGTGTGAATAAACGCATCGGGAACCTGCAGACCGACCTGAGCGACGG GCTACGGCTCATCGCGCTGCTCGAGGTGCTCAGCCAGAAGCGCATGCACCACAAGTACCATCAGCGGCCC ACCTTCAGACAGATGAAGCTGGAGAATGTGTCTGTGGCTCTGGAGTTCCTAGACCACGAAAGCATTAAGC TCGTGTCCATTGACAGTAAAGCCATCGTGGATGGGAACCTGAAGCTCATCTTGGGCCTGGTATGGACTTT GATCCTTCACTACTCCATCTCCATGCCCGTATGGGAAGATGAAGGGGATGATGATGCCAAGAAGCAGACA CCAAAGCAGAGGCTACTGGGATGGATTCAGAACAAGATTCCATACTTGCCCATCACTAACTTTAACCAGA ACTGGCAAGATGGCAAAGCCTTAGGAGCCCTGGTAGACAGCTGTGCTCCAGGTCTGTGCCCAGACTGGGA ATCCTGGGACCCACGGAAGCCGGTGGATAATGCCCGAGAAGCCATGCAGCAGGCCGATGACTGGCTGGGC GTCCCACAGGTCATCACTCCTGAGGAGATTATTCACCCGGACGTGGACGAGCACTCTGTGATGACCTACC TGTCTCAGTTCCCCAAAGCCAAGCTCAAGCCAGGTGCTCCTCTTAAACCCAAACTCAACCCAAAGAAAGC CCGAGCCTATGGCAGAGGAATCGAACCTACTGGAAACATGGTGAAGCAGCCAGCCAAATTCACGGTAGAT ACCATCAGCGCTGGACAAGGAGATGTGATGGTGTTTGTTGAGGACCCAGAAGGAAACAAGGAAGAGGCAC GTGTGACCCCAGACAGTGACAAGAACAAGACATACTCTGTGGAGTATCTGCCCAAAGTCACCGGCCTGCA TAAAGTCATCGTCCTCTTTGCAGGACAGCACATTTCTAAGAGCCCATTTGAAGTGAATGTTGACAAAGCC CAAGGAGATGCCAGCAAAGTCACTGCAAAAGGCCCAGGGCTGGAAACTACAGGGAACATTGCCAACAAGC CTACCTATTTTGACATCTACACAGCTGGAGCTGGCGTGGGGGACATCGGCATTGAGGTGGAGGATCCCCA GGGCAAGAACAGCGTGGAGCTGCTGGTGGAAGACAGAGGAAACCAGGTGTATAGATGTGTCTACAAACCA GTGCAGCCTGGCCCCCATGTGGTCAAGGTCTCCTTTGCTGGGGATGCCATTCCCAAGAGTCCCTTTGGTG TACAAATTGGGGAAGCCTGCAACCCAAATGCCTGCAGAGCCAGCGGCCGAGGTCTGCAGCCCAAAGGTGT CCGGATCCGGGAGACTGCAGACTTTAAGGTGGACACCAAGGCTGCTGGAAGCGGAGAGCTTGGTGTAACT GTGAAGGGCCCTAAGGGCCTGGAGGAGCTGGTCAAACAAAAGGGCTTTCTGGATGGGGTCTACTCATTTG AGTACTACCCCAGCACACCAGGGAAATACAGCGTTGCAGTTACCTGGGGGGGGCACCACATTCCCAAGAG CCCCTTTGAAGTTCAAGTTGGCCCTGAAGCAGGCATGCAGAAAGTCCGTGCATGGGGCCCTGGACTCCAC GGCGGCATTGTTGGTCGGTCAGCAGATTTTGTGGTGGAATCCATTGGTTCTGAGGTTGGAACTCTGGGAT TTGCCATTGAAGGCCCTTCCCAAGCAAAGATCGAGTATGATGATCAGAACGATGGCTCGTGTGATGTCAA ATACTGGCCCAAGGAGCCTGGAGAATACGCCGTTCACATCATGTGCGACGACGAAGACATCAAGGACAGC CCATACATGGCTTTCATCCACCCTGCCACAGGAGACTACAACCCAGATCTAGTCCAAGCCTATGGGCCGG GTTTGGAGAAATCTGGTTGTACTATCAACAACCCAGCAGAGTTCATTGTGGATCCTAAGGATGCCGGGAG TGCTCCCTTAAAGATACTGGCGCAGGATGGGGAAGGCCAGCCCATTGACATCCAGATGAAGAGTCGGATG GACGGCACTTATGCTTGCTCATATACCCCACTTAAAGCCATCAAGCACACCATCGCTGTGGTCTGGGGAG GTGTGAACATACCACACAGCCCCTACAGGGTCAACATTGGGCAAGGTAGCCATCCCCAAAAGGTCAAAGT GTTTGGCCCAGGCGTGGAGAGGAGTGGTCTGAAGGCAAATGAGCCTACTCACTTTACAGTGGATTGCACG GAGGCCGGGGAAGGTGATGTCAGTGTTGGTATCAAGTGCGATGCCCGAGTTTTAAGTGACGATGAGGAAG ATGTCGATTTTGACATTATTCACAATGCCAATGACACATTCACTGTCAAATACGTACCTCCTGCCCCTGG GCGGTATACCATCAAAGTTCTCTTTGCATCTCAGGAAATCCCCGCCAGCCCTTTCAGAGTCAAAGTTGAC CCTTCCCATGACGCCAGCAAGGTGAAGGCAGAAGGCCCCGGGTTGAGTAAAGCAGGCGTGGAAAATGGGA AGCCAACCCACTTCACTGTTCACACCAAGGGAGCCGGAAAAGCTCCGCTCAACGTGCAGTTCAGCAGCCC CCTTCCTGGGGAGGCAGTGAAGGACTTGGATATCATTGATAATTATGACTACTCACATACTGTTAAATAC ACCCCAACCCAGCAGGGCAACATGCAGGTTCTAGTGACTTATGGTGGCGATCCCATTCCTAAAAGTCCTT TCACTGTGGGCGTTGCTGCGCCACTGGATCTGAGCAAGATAAAAATTAATGGACTGGAAAACAGAGTTGA GGTTGGAAAGGACCAGGAATTTGCCATTGACACCAACGGGGCAGGAGGCCAGGGGAAGCTGGATGTGACC ATCCTGAGCCCTTCTCGGAAGGTGGTGCCATGCTTGGTAGCACCAGTGGCTGGCAGGGAATGCAGCACAG CCAAATTCATCCCTCGGGAGGAGGGGCTGTTTGCTGTAGATGTGACCTATGATGGACACCCAGTGCCCGG GAGCCCCTATACTGTGGAGGCCTCACTACCACCAGATCCCACCAAGGTGAAGGCCCACGGTCCTGGCCTT GAAGGAGGTCTTGTAGGCAAGCCCGCTGAGTTCACCATTGACACTAAAGGAGCTGGAACTGGAGGTTTGG GCCTAACTGTGGAAGGTCCATGTGAGGCCAAAATCGAATGCTCGGATAACGGTGACGGGACCTGCTCCGT CTCCTACCTTCCCACAAAACCCGGAGAATACTTTGTCAACATTCTCTTTGAAGAAGTCCACATTCCTGGA TCTCCCTTCAAAGCCGACATTGAAATGCCGTTTGACCCCTCTAAGGTTGTAGCGTCAGGACCAGGACTTG AACACGGAAAAGTGGGTGAACCCGGAATCCTTTGCGTTGATTGTTCAGAAGCAGGGCCTGGGACTCTGGG CCTTGAAGCCGTCTCAGACTCAGGGGCGAAAGCTGAAGTGTCCATCCAGAACAATAAAGATGGCACCTAC GCTGTGACCTATGTGCCTCTGACAGCTGGCATGTACACACTGACCATGAAGTATGGTGGTGAGCTTGTAC CACACTTTCCTGCCTGGGTCAAGGTGGAGCCAGCCATAGACACTAGTGGGATCAAAGCCTTTGGGCCAGG AATCGAAGGCAAGGACGTGTTTCGGGAGGCCACAACGGACTTCACGGTTGACTCGAGGCCCCTTACCCAG GTTGGAGGTGACCATATCAAAGCTCAAATTACCAACCCCTCGGGGGCCTCCACCGAGTGCTTTGTCAAGG ACAATGCTGATGGGACCTATCAGGTCGAATACACGCCCTTTGAGAAAGGTTTCCATGTAGTGGAGGTGAC ATACGATGATGTGCCCATCCCAAATAGCCCATTCAAAGTGGCTGTCACTGAAGGCTGCCAGCCATCTCGG GTCCATGCGCAAGGCCCTGGACTGAAAGAGGCGTTTACCAACAAATCTAATGTCTTCACAGTGGTAACCA GGGGAGCAGGGATCGGTGGGCTTGGCATAACTGTGGAGGGGCCGTCGGAGTCAAAGATAAACTGCAGAGA CAACAAGGATGGCAGCTGCAGTGCTGAGTACATTCCTTTCGCTCCAGGGGATTATGACGTCAATATCACG TACGGAGGCGTCCACATCCCTGGCAGCCCCTTCAGAGTTCCTTCAAAAGATGTTGTGGATCCCAGCAAGG TCAAGATTGCTGGCCCCGGTCTGAGCTCCTGTGTCCGAGCATGCATCCCGCAGTCCTTCACAGTGGACAG CAGCAAGGCTGGCCTAGCTCCCCTGGAAGTGAGGGTCCTGGGCCCAAGAGGCCTCGTGGAGCCGGTGAAT GTGGTAGATAATGGGGACGGCACCCACACAGTGACCTACACGCCATCTCAGGAGGGGCCCTACATTGTTT CCGTCAAATATGCTGATGAAGAGATCCCCCGAAGTCCCTTTAAGGTCAAGGTTCTTCCCACATACGATGC AAGCAAAGTGACAGCCTCTGGGCCTGGCCTCAGCGCCTATGGGGTACCCGCCAGCCTACCTGTGGAGTTC GCCATTGATGCCAGAGATGCTGGCGAAGGCCTGCTTGCTGTCCAGATCACGGACCAGGAGGGAAAGCCTC AAAGAGCTACTGTCCACGACAACAAAGATGGCACATATGCCGTTACCTATATCCCTGATAAGACCGGACG CTATATGATTGGAGTCACCTATGGTGGAGACAACATTCCACTTTCTCCTTACCGTATCCGGGCCACACAG ACAGGCGATGCCAGCAAGTGCTTGGCCACAGGTCCTGGTATTGCCCCCACTGTGAAAACTGGCGAGGAGG TGGGCTTTGTAGTGGACGCTAAGACTGCAGGGAAGGGGAAAGTAACCTGCGTGATTCTGACCCCAGATGG CACCGAGGCTGAGGCTGACGTCATTGAAAATGAAGATGGCACCTATGACATTTTCTACACTGCTGCCAAG CCAGGCACCTATGTTATCTATGTGCGCTTTGGTGGCGTTGATATTCCCAATAGCCCCTTCACAGTCATGG CCACCGATGGGGAAGTCACAGCCATGGAGGAGGCACCGGTAAATGCATGTCCCCCTGGATTCAGGCCCTG GGTTACAGAAGAGGCCTATGTCCCTGTGAGTGACATGAACGGGCTAGGCTTCAAGCCCTTTGACTTGGTG ATTCCATTTGCCGTCAGGAAAGGAGAAATTACAGGCACGGTGCATATGCCTTCTGGGAAGAAGGCTACAC CTGAGATTGTGGACAACAAGGACGGCACGGTCACTGTGAGATACGCGCCCACTGAAGTCGGCCTCCACGA GATGCACATCAAGTACAGGGGCAGCCACATCCCGGAGAGCCCCCTGCAGTTCTACGTGAACTACCCCAAC AGTGGGAGTGTGTCTGCTTATGGTCCAGGCCTTGTGTATGGAGTAGCCAACAAAACTGCCACCTTCACCA TTGTCACAGAGGATGCGGGAGAAGGTGGTCTGGACTTGGCTATTGAAGGACCCTCGAAAGCAGAGATCAG CTGCATTGACAACAAAGACGGGACATGTACAGTGACCTACCTACCCACCTTGCCAGGGGACTACAGCATT CTGGTCAAGTACAATGACAAACATATTCCTGGCAGCCCCTTCACTGCCAAGATCACAGATGATAACAGAC GCTGCTCCCAAGTGAAGCTGGGCTCAGCTGCTGACTTCCTTCTGGACATCAGTGAGACAGACCTTAGCAC ACTGACAGCCAGTATCAAGGCCCCATCTGGACGTGACGAGCCCTGTCTGCTAAAGAGGCTACCCAACAAC CACATTGGCATTTCCTTCATCCCCCGAGAAGTGGGTGAGCATCTAGTCAGCATCAAGAAAAACGGTAACC ACGTGGCCAACAGCCCTGTGTCCATTATGGTGGTCCAGTCTGAAATTGGTGATGCACGCCGAGCCAAAGT CTATGGCCAAGGCCTGTCAGAAGGCCGGACTTTTGAGATGTCAGACTTCATCGTGGACACAAGAGATGCA GGCTATGGTGGCATATCCTTGGCAGTAGAAGGACCCAGCAAGGTGGACATCCAAACAGAGGACCTGGAGG ACGGCACCTGCAAGGTGTCCTACTTCCCCACTGTGCCCGGGGTCTATATCGTCTCCACCAAATTTGCTGA CGAGCATGTGCCTGGAAGCCCATTCACTGTGAAGATCAGTGGCGAGGGAAGAGTCAGAGAGAGCATCACC CGGACCAGCCGGGCTCCGGCTGTGGCCACGGTTGGCAGTATCTGTGACCTGAACCTGAAGATCCCGGAGA TCAACAGCAGTGACATGTCTGCCCATGTCACCAGCCCCTCTGGCCATGTCACTGAGGCAGAGATTGTGCC CATGGGAAAGAACTCTCACTGTGTCAGATTTGTGCCCCAGGAGATGGGCGTTCACACTGTCAGCGTAAAG TACCGCGGACAGCACGTAACAGGAAGCCCGTTCCAGTTCACCGTGGGGCCCCTTGGCGAAGGGGGTGCCC ACAAGGTCCGGGCAGGAGGCCCTGGCCTGGAGAGGGGAGAAGCAGGCATCCCAGCTGAGTTCAGCATCTG GACCCGGGAAGCAGGTGCTGGCGGCCTCTCCATTGCTGTTGAGGGCCCTAGTAAGGCCGAGATCACATTC GATGACCATAAAAATGGGTCATGCGGTGTGTCTTATATTGCCCAAGAGCCTGGTAACTACGAGGTGTCTA TCAAGTTCAACGACGAGCACATCCCGGACAGCCCTTACCTGGTGCCCGTCATCGCGCCCTCAGACGACGC TCGCTGCCTCACTGTTCTGAGCCTTCAGGAATCAGGATTAAAAGTTAACCAGCCAGCGTCCTTTGCTATA AGGTTGAATGGTGCGAAAGGGAAGATTGATGCAAAGGTGCACAGCCCCTCAGGAGCCGTGGAGGAATGCC ACGTGTCTGAACTGGAGCCAGATAAGTATGCCGTTCGCTTCATCCCGCATGAGAATGGCATCCACACGAT CGATGTCAAGTTTAACGGGAGCCACGTGGTCGGGAGCCCTTTCAAAGTACGTGTAGGAGAGCCTGGCCAG GCAGGCAATCCTGCTCTAGTGTCAGCCTACGGTGCTGGGCTTGAGACGGGCACCACAGGTATCCAATCAG AATTCTTCATCAATACCACTCAGGCAGGCCCAGGGACATTATCTGTCACCATTGAAGGACCATCCAAGGT CAAAATGGATTGCCAGGAAATCCCAGAGGGGTACAAAGTCATGTACACTCCCATGGCTCCTGGAAATTAC CTGATTGGTGTCAAATATGGCGGGCCCAACCACATCTCGAGGAGTCCATTCAAGGCCAAGGTGACAGGCC AACGTCTGGTCAGCCCAGGCTCAGCCAACGAGACCTCCTCCATCTTGGTGGAGTCCGTAACCAGATCCTC AACGGAAACGTGCTATAGCGCCATTCCCAAGTCGTCCTCCGATGCCAGCAAAGTGACCTCCAAGGGCGCA GGCCTGTCAAAGGCTTTTGTGGGCCAGAAGAGTTCTTTCCTGGTGGACTGCAGCAAAGCTGGGTCCAACA TGCTGTTGATTGGGGTCCATGGGCCCACCACCCCCTGTGAAGAGGTCTCCATGAAGCATGTGGGCAAGCA ACAGTACAACGTCACCTACGTCGTCAAGGAGAGGGGCGATTACGTTCTGGCTGTGAAGTGGGGGGAAGAG CACATCCCCGGTAGCCCCTTTCATGTCACCGTGCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081427
Insert Size	7809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081427.1, NP_001074896.1
RefSeq Size	9118 bp
RefSeq ORF	7809 bp
Locus ID	286940
UniProt ID	Q80X90
Gene Summary	Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This gene lacks full-length transcript support, and therefore the exon combination of this variant is inferred based on tiled partial transcripts and full-length orthologous alignments, e.g., human AF043045.1. CCDS Note: The exon combination of this CCDS representation is inferred due to a lack of full-length transcript support for this gene. It is supported by tiled partial transcripts and full-length orthologous alignments (e.g., human AF043045.1).

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