Col6a3 (NM_001243009) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI507288; Col6a-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229759 representing NM_001243009 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAACACCGGCATCTGCCCCTAGTGGCTGTCTTTAGCCTTCTCCTCTCAGGCATTGCCACGACTC ATGCCCAACAGCATGGAGTCATGGAAGTCAACAAGAGGGACATAGTCTTCCTGGTGGATGGTTCCTCCAG CCTGGGGCCATCCAACTTCAATGCCATCCGAGACTTCGTTACCAGAGTCATCCAGAGGTTGGAAATTGGA CAGGACCTGGTTCAGGTATCAGTGGCTCAGTATGCAGACACTGTCAAGCCTGAGTTCTATTTAAATTCCT ATACCAACAAAAGGGATGCCATAACTGCTGTACGGAAGATGAGGGCCCTGAATGGCTCAGCCCTGTACAC GGGATCTTCTCTGGACTTTGTTCGAAACAACCTCTTCACTAGCTCAGCCGGCCATAGGGCCGCTGAGGGG GTTCCTAAGCTCCTGGTGCTGATCACAGGCGGTAAGTCCCTCGATGAAGTCAGCCAGCCTGCCCAGGAGC TGAAGAGAGGCAGCATCATGGCCTTGGCCGTTGGCAGCAAGGCCGCCGATGAGGATGAGCTGAAAGAGAT CGCTTTCGACTCCTCCCTGGTCTTCATTCCCGCTGAGTTCCGCCCTGCTCCCTTGCAAAACATGCTGCCC AGCCTGATGGCACCTCTCAGGACTCTCACTGGAACCACGGAAGAGAGCAAGCGGGACATTCTTTTCCTCT TTGACGGCTCAGTCAATGTCTTGGGTCAGTTCCCTGCTGTCAGAGACTTTCTCTACAGGATCATTGAGGA ACTTGATGTGAAGCCGGATGGGACCCGGGTAGCAATAGCTCAGTTCAGTGATGACGTCAGGCTGGAGTCC CGCTTCAGTGAGCACCAGACGAAAGCTGAAATCCTTAATCTCGTGAAGAAGATGAAGCTAAAAACAGGCA AAGCCCTCAACCTGGGCTACGCCCTGGACTACGCGCTTAGGAACATCTTTGTGAGGTCGGCTGGCAGCCG CATCGAGGATAACGTGCAGCAGTTCCTGGTGCTGCTGGTTGCTGGAAGGTCGTCAGATGCTGTGGCCGGG CCTGCAAGCTCCCTGAAGCAGAGAGGAGTGGTACCTTTCATCTTTCAAGCCAAGAACGCCAACCCCAGTG AGCTGGAGCAGATTGTGCTGTCGCCTGCATTCATCCTGGCTGCAGAGTCACTCCCTAAGATCGGAGACCT CCAATCACAGATTGTGAGCCTTCTGAAAGCAGAACAGGGTTCAGGCCCTGTATCAGGTGAAAAGGATGTG GTGTTTCTCATTGATGGCTCTGAGGGTGTCCGCAGTGGCTTCCCCCTGCTAAAGGACTTTGTGCAGAGGG TTGTGGAGAGCTTGGATGTGGGTCCCGACCGTGTGCGTGTGGCACTGGTGCAGTACAGTGACCGGACCAG GCCGGAGTTCTACCTGAATTCCCACATGGACCAGCAGGGTGTCATCAGCGCCATCCGCAGGCTGACACTG CTGGGCGGCCCAACCCCCAACACAGGGGCGGCACTGGAGTTCGTGTTAAGGAACATTCTGACCAGTTCTA CTGGGAGCAGGATAGCAGAAGGTGTTCCTCAGCTCCTGATTGTCCTCACAGCAGAGCCATCAGGGGATGA CGTGCGAGGCCCTTCAGTAGTCCTGAAGCAGGGCGGGGCTGTGCCCATTGGCATTGGCATTGGGAATGCT GACATCTCTGAAATGCAGACCATCTCCTTCATCCCTGACTTTGCTGTGGCCATCCCCACCTTCCGGGAGC TTGGGACGATACAGCAGGTCATCTCTGAGAGGGTGATCCAGCTTAATCGTGAGGAGCTGAGCTCGTTGAA ACCCATTTTGACGCCCTCAACAGGTGCAGGTGTTGGCAGCAAGAAAGATGTGGTCTTTCTCATCGATGGA TCCCGAAATGCTGGCCCTGAGTTCCAGTACATCCGTACCCTGATTGAGAGGATAGTCGAGTACCTGGACA TAGGCTTTGACACCACCCGGGTAGCCGTCATCCAGTTCAGTGAGGACTCCAAGATGGAGTTTCCTCTGAA TGCCCACTTCAGCAAGGATGAAGTACAGAACGCTGTGCGCAGGCTGCGGCCCAAGGGTGGGAGTCAGGTC TACATCGGAAATGCCCTGGAGTACGTGCTGAAGAACATCTTCCAGAGGCCACTGGGGAGCCGGATAGAAG AGGGCGTTCCGCAGTTCCTAGTCCTCATTTCGTCTGGAAAGTCTGATGATGAGGTGGACGACTCAGCCGT GGAACTCAAGCAGTTCGGTGTGGCCCCTCTCACCATAGCCAGACACACAGACCAAGAGGAGCTGGTGAAG ATCTCCCTAAGTCCTGAGTACGTGTACTCCGTGAGCACCTTCAGGGAGCTGCCCAGACTGGAGCAGAAGC TGCTGACGCCCATCACCACTCTAACCTCACAGCAGATCCATCAGATCCTGGCCAGTACCCGATATCCCCC TTCAGTGGTTGAAAGCGATGCGGCTGACATCGTCTTTCTGATTGACAGCTCTGATGCCGTCAAGCCCGAT GGCATCGCTCATATCCGAGACTTTGTCAGCAGGATCGTTCGCAGACTCAACATTGGTCCCAGTAAAGTGA GGATTGGGGTCGTGCAGTTTAGCAATGATGTCTTCCCTGAGTTCTACCTGAAGACCCACAAGTCCCAGAG CAGTGTGCTTGAAGCCATACGGCGTCTGAGGTTCAAAGGAGGGTCTCCCCTAAACACTGGCAGAGCCCTG GAGTTTGTGGCAAGAAACCTCTTCGTGAAGTCTGCTGGGAGCCGGATAGAAGATGGGGTCCCTCAACACC TGGTCTTATTCCTGGGTGGGAAGTCTCAGGACGATGTGGCCAGGCATGCCCAAGTCATAAGCTCATCGGG GATTGTGAGCTTAGGAATAGGAGATCGGAACATTGATAGGACGGACTTGCAAACCATCACCAATGACCCC AGGCTGGTCTTCACAGTTCGGGAATTCAGAGAGCTGCCCAACATAGAAGAGAGGGTCATGCTTTCCTTTG GACCCTCTGGGGCTACTCCTCAGCCTCCTGGGGTAGATTTACCTTCTCCTTCCCGGCCAGAGAAGAAGAA GGCCGACATTGTGTTCCTGCTGGATGGTTCCATCAATTTTAGGAGGGACAGTTTCCAGGAAGTGCTCCGT TTTGCATCTGAAATCGTGGACACGGTCTATGAAGACGGAGATTCCATCAGAGTGGGACTGGTCCAGTACA ACTCAGACCCCACGGATGAATTCTTCCTGAGGGACTTTTCTACCAAAAGGCAGATTATCGATGCCATCAA CAAAGTCGTCTATAAAGGAGGGAGGCATGCCAACACCAGGGTGGGCATCGAGCACCTGCTGCGGAATCAC TTTGTGCCGGAGGCTGGTAGCCGCCTGGATGAGCGGGTTCCTCAGATCGCCTTCGTGATCACCGGAGGGA AGTCAGTGGAGGATGCTCAGGATGTGAGCCTGGCTCTCACCCAGAAAGGTGTCAAGGTGTTTGCCGTTGG CGTGAGGAACATCGACTCTGAGGAGGTGGGCAAGATAGCCTCCAACAGTGCCACAGCCTTCCGGGTGGGC AGCGTGCAGGAACTGTCCGAACTGAGTGAGACAGTGCTCGAGACTCTGCATGATGCAATGCATGAGACCC TCTGCCCTGGTGTGACCGATGTCTCCAAAGCCTGCAATCTGGAAGTCATTCTGGGGTTTGATGGATCTCG AGATCAGAATGTGTTTGTGAGTCAGAAGGGCCTTGAGTCCAAGGTGGACATCATCTTGAACAGAATCAGC CAGATACAAAGGATCAGCTGCAGTGGCAACCAGCTGCCCACTGTGAGGGTATCCGTGATGGCGAACACGC CCTCTGGGCCAGTGGAGGCCTTTGACTTTGCCGAGTATCAGCCGGAGCTGTTTGAGAAGTTTCGCAACAT GCGCAGTCAGCGCCCATACGTCCTCACAGCTGACACGCTGAAGTTGTACCAGAACAAGTTCAGGCAGTCG TCGCCTGATACTGTGAAGGTAGTCATTCACTTCACGGATGGAGCAGATGGAGACATGGCTGACTTATACA GAGCATCTGAGGAACTCCGGCAAGCAGGTGCCCAAGCCCTGATCCTGGTGGGTCTTGAACGTGTGGCTAA CCTGGAGCGGCTGATGCATCTGGAGTTCGGGCGGGGCTTCATGTATGACAGGCCCCTGAGGCTCAACCTG CTTGACTTAGACTATGAACTGGCAGAGCAGCTTGACAATATTGCTGAGAAAGCTTGCTGTGGGGTTCCAT GCAAGTGCTCTGGAGAGAGGGGAGACAGAGGGCCCATTGGCAGCATTGGGCCAAAGGGTATCTCCGGGGA AGATGGCTACCGAGGCTACCCGGGAGACGAAGGCGGACCTGGTGAGCGAGGTCCACCTGGTGTGAATGGC ACACAAGGTTTCCAGGGCTGTCCAGGCCAGAGGGGAGTGAAGGGTTCTCGAGGATTCCCAGGAGAGAAGG GTGAATTGGGAGAAATCGGCTTGGATGGTCTGGATGGTGAAGAGGGAGACAAGGGGCTGCCTGGTTCTTC TGGAGAAAAGGGGAGTCCTGGAAGAAGGGGTGACAAAGGACCCAAAGGAGACAAAGGCGAGAGAGGAGAT GTCGGGATTCGAGGTGACCCGGGTGACTCAGGACGGGACAGCCAGCAGAGAGGACCCAAAGGAGAGACGG GAGACATCGGCCCCATGGGTCTCCCAGGCAGAGATGGCATCCCTGGCAGCCCAGGAGACCCTGGGAAGGA CGGTGGCTCTGGCCGAAGGGGCCCTGCAGGAGCTAAGGGCAACAGAGGTGGTCCTGGCCAGCCAGGCTTC GAAGGCGAGCAGGGTACCAGAGGCTCACAGGGCCCACCTGGTCCCATTGGTCCCCCAGGCCTGATTGGGG AACAAGGAATTCCCGGACCTCGAGGAGGCGGAGGTACGGCTGGGGCTCCTGGAGAACGTGGCAGAACTGG TCCTCTGGGGAGGAAGGGTGAGCCAGGAGAGCCAGGGCCGAAGGGAAGCATCGGGAACCGTGGACCCCGA GGAGAGACGGGAGATGACGGGAGAGATGGGGTTGGCAGTGAAGGACGCAGAGGCAAAAAAGGAGAAAGAG GCTTCCCTGGGTATCCGGGACCAAAGGGCACCCCTGGTGAGCCAGGGGCAGATGGACCACCAGGACCCAA AGGCATCAGAGGTCGAAGGGGAAATTCAGGACCTCCAGGGGCAACTGGACAAAAGGGAGACCCTGGTTAC CCAGGACCATCTGGTCACAAGGGCAACCGAGGCGACTCCGTTGATCAATGCGCCCTCATCCAGAGCATCA GAGACAAATGCCCTTGCTGCTACGGGCCCCTGGAGTGCCCTGTATTCCCAACAGAACTCGCCTTTGCCCT GGATACCTCTGAGGGGGTGACTCAGGACACCTTCAGCCGGATGCGAGAGGTGCTTCTGGGCATTGTGGGC GATCTGACCATTGCAGAGAGCAACTGTCCGCGGGGTGCCCGCGTGGCTGTGGTCACCTATAACAATGAGG TGACTACAGAGATCCGGTTTGCTGACTCCAAGAAGAAGTCTGCCCTCTTAGACAGTATTCAGAACCTCCA AGTGGCCTTGACCTCTAAGCAGCAGAGCCTGGAGACCGCAATGTCATTCGTGGCCAGGAATACATTCAAA CGTGTGAGGAGTGGCTTCCTGATGAGGAAAGTGGCTGTTTTCTTCAGCAACAAGCCCACCAGGGCATCCC CCCAGCTCCGAGAGGCCGTACTCAAGCTTTCCGATGCGGGGATCACACCCTTGTTCCTTACAAGCCAGGA GGACCGACAGCTCATCAACGCTTTGCAGATCAATAACACAGCAGTGGGACATGCCCTGGTTCTGCCTGCA AGGAGGGATCTCACAGACTTCCTGAAGAATGTCCTTACCTGCCATGTTTGCTTGGACATTTGCAATATCG ACCCATCCTGTGGCTTCGGCAGCTGGAGGCCTTCCTTCAGGGACCGGAGGGCAGCAGGCAGTGATGTGGA CATAGACCTGGCTTTCATCTTGGACAGCTCAGAGGCTACCACACTGTTCCAGTTCAATGAGATGAAGAAA TACATAGGCTATGTGATCAGACAGCTGGACCTGAGCCCAGACCCGAAGGCTTCCCAACACTTTGCCAGGG TGGCTGTTGTGCAGCAGTCAACCTACGAGTCTGTGGACAATGCCAGTGTGCCGCCTGTGAAGGTGGAATT CTCACTGACAGACTATGGTGCCAAGGAAAAGCTGTTGGATTTCCTTAGCCGGAGGATGACCCAACTTCAG GGAACCATGGGCCTGGGCAATGCCATTGAATACACTATAGAGAACATCTTTGAAAGTGCTCCAAACCCAC GGGACCTCAAAATTATGGTGCTGATGTTGACTGGTGACATGCAGAGGCAGCAGCTGGAGGAGGCCCAGAG AGCCATCCTCCAGGCCAAGTGCAAGGGTTACTTCTTTGTGGTTCTGGGCATTGGCAGGAAGGTGAATATC AAGGAGGTCTACAGCTTTGCCAGTGAGCCCAATGATGTCTTCTTCAAATTTGTGGACAAATCAACTGAGC TCAACGAGGAGCCTCTGATGCGCTTTGGGAGGCTGCTCCCATCCTTTGTCAGCAGTGAAAATGCATTTTA CCTGCCTCCAGATCTCAGGAAACAGTGTGATTGGTTCCAAGGGGACCAACCAGTGAAGAATGGCGTGAAG TTTGGTCACAAACAGATAAACCCTCCACATACTGCTAATTCAAGTCTTACATCCAAAGTTGTGACCACAA TGAAGCCAGTGACAACGACCAAACCAACAGCAATTGTAAACCTGCCACCTGCTAAGCCAGCCCCAGCGAG GCCAGCCCCTGCTCAGCCAGTTCTTGCTAAGCCAGACCCCGCAAAACCAGCCCAAGCAAGGCCAGCCCCT GCTAAGCCAGCTTCTGCTAAGTTGGTGCCTCCTCAGCCAGTCCATGTTCAGCCAGCCCCTGCCCAGACAG CTTCTGTAAGACCAGCCCCCGCTAAGCCAGCCCCTCCTCAGCCAGCTGCTGCTAAGCCAGTCCCTGCTAA GCCAGCAGTCCCTGCTCAGCCAGCCCCTCCTCAGCCAGCTGCTGCTAAGCCAGTCCCTGCTAAGCCAGCA GTCCCTGCTCAGCCAGCCCCTCCTCAGCCAGCTGCTGCTAAGCCAGTCCCTGCTAAGCCAGCAGTCCCTG CTCAGCCAGCCGCTGCTCAACCAATGCCTGCTCAGCCAGTCCTTACTAAATCAGCCGCTGTGAAACCAGC CTCTGCCAACAAGCCTGTGGCAGCCAAACCTGTGGCCACGAACACAGCCACAGCCACAGCCAGACCTGCA TTAGCAGCGAAGCCTGCTGCAGCGAAGCCTGCAGCAACGAGACCCCTTGCTGCTGCCATAAGGCCAGTTG CTACCAAGCCAGAGGCCCCCAGGCAACAGGCCAAACCAGCTGCCACCAAGCCAGCCACTACTAAGCCCTT GGCGAGAGTGTCCCGAGAAGTCCAGGTCTCTGAAGTCACGGAGAACAGTGCCAGACTCCACTGGGAGCGG CCCGAGCCTTCCAGCTCCTTTTTTTATGACCTCACTGTAACCTCGGCCCACGACCAGTCCTTGGTTCTGA GACAAAACCTCACTGTCACTGACCGTGTCATCGGAGGCCTACTGGCTGGACAGCTGTACCATGTCGTAGT GGTCTCCTACCTGCAGTCTCAGGTCAGGGCCATCTACCAAGGAAGTTTCAATACGAAGAAAACCCAGCCT CCACCTCTTCAGGCAGCACACCGAGCATCCAGTTCAACCATCAACCTCATGGTGAACACAGAACCATTGT TTCTCACTAAAACAGATATATGTAAGCTGTCCAGAGATGCTGGGACTTGTGTGGACTTCAAGTTACTATG GCACTATGACCTAGAGAGCAAAAGTTGCAAGAGATTCTGGTATGGAGGTTGTGGAGGCAACGAGAACAGA TTCCACTCCCAGGAAGAATGTGAAAAGATGTGTAGTCCTGAGTTAACAGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243009
Insert Size	8034 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243009.1, NP_001229938.1
RefSeq Size	9024 bp
RefSeq ORF	8034 bp
Locus ID	12835

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