Jazf1 (BC048577) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Tip27, C820002C15, Jaz1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201591 representing BC048577 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGATGCTGCACGCCGAGAACAGGAATCTCTGAAGAAGAAGATTCAGCCGAAGCTTTCACTAACCC TGTCCAGCTCCGTGTCTCGAGGGAATGTGTCCACTCCACCTCGACATAGCAGTGGCAGCCTTACTCCCCC TGTGACCCCGCCCATCACGCCCTCCTCTTCATTCCGCAGCAGCACTCCAACAGGCAGCGAGTATGATGAG GAAGAGGTGGACTATGAGGAGTCAGACAGTGATGAGTCCTGGACCACAGAGAGCGCCATCAGCTCTGAAG CAATCCTCAGCTCCATGTGCATGAATGGAGGGGAAGAGAAGCCTTTCGCCTGCCCAGTTCCAGGGTGTAA AAAGAGATACAAGAATGTGAATGGCATAAAGTACCATGCTAAGAATGGTCACCGAACACAGATTCGCGTC CGCAAACCATTCAAATGCCGGTGTGGGAAGAGTTACAAGACAGCTCAGGGCCTGCGGCACCACACAATCA ATTTCCATCCCCCAGTGTCTGCCGAGATGATCAGGAAGATGCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201591 representing BC048577 Red=Cloning site Green=Tags(s) MTDAARREQESLKKKIQPKLSLTLSSSVSRGNVSTPPRHSSGSLTPPVTPPITPSSSFRSSTPTGSEYDE EEVDYEESDSDESWTTESAISSEAILSSMCMNGGEEKPFACPVPGCKKRYKNVNGIKYHAKNGHRTQIRV RKPFKCRCGKSYKTAQGLRHHTINFHPPVSAEMIRKMQQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC048577
ORF Size	539 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC048577, AAH48577
RefSeq Size	949 bp
RefSeq ORF	539 bp
Locus ID	231986
Gene Summary	Acts as a transcriptional corepressor of orphan nuclear receptor NR2C2 (By similarity). Inhibits expression of the gluconeogenesis enzyme PCK2 through inhibition of NR2C2 activity (PubMed:24380856). Also involved in transcriptional activation of NAMPT by promoting expression of PPARA and PPARD (PubMed:24930994). Plays a role in lipid metabolism by suppressing lipogenesis, increasing lipolysis and decreasing lipid accumulation in adipose tissue (PubMed:24380856, PubMed:25614086). Plays a role in glucose homeostasis by improving glucose metabolism and insulin sensitivity (PubMed:25614086, PubMed:24380856).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.