Anapc10 (NM_026904) Mouse Tagged ORF Clone
CAT#: MG201723
- TrueORF®
Anapc10 (tGFP-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10)
ORF Plasmid: DDK
"NM_026904" in other vectors (3)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1500026N15Rik; A830003M23Rik; APC10 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201723 representing NM_026904
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACACCGAACAAGACACCTCCTGGTGCTGATCCTAAGCAGTTGGAGAGGACAGCAACAGTCAGGG AAATTGGGTCTCAGGCTGTTTGGTCACTCTCTTCCTGCAAACCAGGGTTTGGAGTGGATCAATTACGGGA TGACAATCTAGAAACTTACTGGCAATCTGATGGCTCTCAGCCTCACTTAGTGAACATCCAATTCAGAAGA AAAACAACAGTGAAGACATTATGTATTTATGCAGACTATAAGTCTGATGAAAGCTACACTCCAAGCAAAA TCTCAGTCAGAGTAGGAAATAATTTTCACAACCTTCAAGAAATCCGGCAACTTGAATTGGTGGAACCAAG TGGCTGGATTCACGTTCCTTTAACTGACAATCATAAGAAGCCAACTCGCACATTCATGATACAGATTGCT GTTCTAGCCAATCATCAGAATGGAAGAGATACCCACATGAGACAAATTAAGATATACACACCAGTGGAAG AGAGCTCCATTGGGAAATTCCCTAGATGCACAACCATTGATTTCATGATGTATCGTTCAATACGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201723 representing NM_026904
Red=Cloning site Green=Tags(s) MTTPNKTPPGADPKQLERTATVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQPHLVNIQFRR KTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHKKPTRTFMIQIA VLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026904 |
ORF Size | 555 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026904.3 |
RefSeq Size | 2802 bp |
RefSeq ORF | 558 bp |
Locus ID | 68999 |
UniProt ID | Q8K2H6 |
Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR201723 | Anapc10 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10) |
CNY 2,400.00 |
|
MR201723L3 | Lenti ORF clone of Anapc10 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10) |
CNY 4,750.00 |
|
MR201723L4 | Lenti ORF clone of Anapc10 (mGFP-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10) |
CNY 4,750.00 |
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