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Ncs1 (NM_019681) Mouse Tagged ORF Clone
CAT＃: MG201801
- TrueORF®
Ncs1 (tGFP-tagged) - Mouse frequenin homolog (Drosophila) (Freq)
ORF Plasmid: DDK
"NM_019681" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9430075O15Rik; A730032G13Rik; AI836659; Freq; Mfreq; NCS-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201801 representing NM_019681
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAATCCAACAGCAAGTTGAAGCCTGAAGTTGTGGAGGAGCTGACCAGAAAAACCTACTTCACTG AGAAAGAAGTACAGCAGTGGTACAAGGGCTTCATTAAGGACTGCCCCAGCGGGCAGCTGGATGCCGCTGG CTTCCAGAAGATCTACAAGCAGTTCTTCCCATTTGGAGACCCCACCAAGTTCGCCACGTTTGTTTTCAAC GTCTTCGACGAGAACAAGGATGGCAGGATTGAGTTCTCCGAGTTCATCCAGGCACTGTCGGTGACCTCAA GGGGGACCCTGGATGAAAAGTTGCGATGGGCCTTCAAGCTTTATGACTTGGATAACGATGGCTACATCAC CAGGAACGAGATGCTCGACATAGTGGACGCCATTTACCAGATGGTGGGGAACACCGTGGAGCTCCCAGAA GAAGAGAACACACCAGAGAAGAGGGTGGACCGGATCTTTGCCATGATGGACAAGAATGCTGATGGGAAGC TGACTCTTCAGGAGTTCCAGGAAGGCTCCAAGGCTGATCCCTCCATTGTGCAGGCGCTGTCCCTCTACGA TGGGCTGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201801 representing NM_019681
Red=Cloning site Green=Tags(s) MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFN VFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019681 |
| ORF Size | 570 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019681.2 |
| RefSeq Size | 4036 bp |
| RefSeq ORF | 573 bp |
| Locus ID | 14299 |
| UniProt ID | Q8BNY6 |
| Gene Summary | Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202420 | Ncs1 (untagged) - Mouse neuronal calcium sensor 1 (Ncs1), (10ug) |
CNY 2,400.00 |
|
| MR201801 | Ncs1 (Myc-DDK-tagged) - Mouse neuronal calcium sensor 1 (Ncs1) |
CNY 2,400.00 |
|
| MR201801L3 | Lenti ORF clone of Ncs1 (Myc-DDK-tagged) - Mouse neuronal calcium sensor 1 (Ncs1) |
CNY 4,750.00 |
|
| MR201801L4 | Lenti ORF clone of Ncs1 (mGFP-tagged) - Mouse neuronal calcium sensor 1 (Ncs1) |
CNY 4,750.00 |
