Rbpms (BC030397) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2010300K22Rik; 2700019M19Rik; AU017537; hermes; RBP-MS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201952 representing BC030397 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGCGGCGGCAAAGCCGAGAAGGAGAACACCCCGAGCGAGGCCAACCTTCAGGAGGAGGAGGTCC GGACCCTATTTGTCAGCGGTCTGCCTCTGGACATCAAGCCCCGAGAGCTGTACCTGCTCTTCAGACCATT CAAGGGCTATGAAGGTTCTCTCATAAAGCTCACATCTAAACAGCCCGTAGGCTTTGTCAGTTTTGACAGT CGCTCAGAAGCAGAGGCCGCAAAGAACGCTTTGAACGGCATCCGCTTCGATCCTGAAATCCCGCAAACGC TACGACTAGAGTTTGCTAAGGCAAACACGAAGATGGCCAAGAACAAACTCGTAGGGACTCCAAACCCCAG TACTCCTCTGCCCAACACTGTACCTCAGTTCATTGCCAGGGAGCCATATGAGCTCACAGTACCTGCACTT TACCCCAGTAGCCCTGAAGTTTGGGCCCCGTACCCTCTGTACCCAGCGGAGTTAGCGCCTGCTCTTCCTC CTCCTGCCGCTTTCACCTACCCCGCTTCACTGCATGCCCAGATGCGCTGGATCCCTCCCTCCGAGGCCAC TTCCCAGGGCTGGAAGTCCCGGCAGTTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201952 representing BC030397 Red=Cloning site Green=Tags(s) MNGGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDS RSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPAL YPSSPEVWAPYPLYPAELAPALPPPAAFTYPASLHAQMRWIPPSEATSQGWKSRQFC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC030397
ORF Size	593 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030397, AAH30397
RefSeq Size	1124 bp
RefSeq ORF	593 bp
Locus ID	19663
Gene Summary	Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.[UniProtKB/Swiss-Prot Function]

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