Thap1 (NM_199042) Mouse Tagged ORF Clone
CAT#: MG202256
- TrueORF®
Thap1 (tGFP-tagged) - Mouse THAP domain containing, apoptosis associated protein 1 (Thap1)
ORF Plasmid: DDK
"NM_199042" in other vectors (3)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4833431A01Rik; AW490810 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202256 representing NM_199042
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGTCCTGCTCCGCCTACGGCTGCAAGAACCGCTACGACAAGGACAAGCCCGTCTCCTTCCACA AGTTTCCTCTTACTCGCCCCAGCCTTTGTAAGCAGTGGGAGGCAGCTGTTAAAAGGAAAAACTTCAAGCC CACCAAGTACAGCAGCATCTGCTCGGAGCACTTCACCCCGGACTGCTTTAAGAGGGAGTGCAACAACAAG CTACTGAAGGAGAACGCTGTGCCCACAATATTTCTCTATATCGAGCCACATGAGAAGAAGGAAGACCTGG AATCCCAAGAACAGCTCCCCTCTCCTTCACCCCCCGCTTCCCAGGTTGATGCTGCTATTGGGCTGCTAAT GCCCCCTCTGCAGACCCCTGATAACCTGTCGGTTTTCTGTGACCACAATTACACTGTGGAGGATACGATG CACCAGAGGAAGAGGATCCTGCAGCTGGAGCAGCAGGTGGAGAAACTCAGGAAGAAGCTCAAGACGGCCC AGCAGCGGTGCCGGCGGCAGGAGAGGCAGCTCGAGAAGCTCAAGGAAGTCGTCCACTTTCAGAGAGAGAA GGACGACGCGTCCGAGAGGGGCTACGTGATCCTACCAAATGACTACTTTGAAATTGTTGAAGTTCCAGCA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202256 representing NM_199042
Red=Cloning site Green=Tags(s) MVQSCSAYGCKNRYDKDKPVSFHKFPLTRPSLCKQWEAAVKRKNFKPTKYSSICSEHFTPDCFKRECNNK LLKENAVPTIFLYIEPHEKKEDLESQEQLPSPSPPASQVDAAIGLLMPPLQTPDNLSVFCDHNYTVEDTM HQRKRILQLEQQVEKLRKKLKTAQQRCRRQERQLEKLKEVVHFQREKDDASERGYVILPNDYFEIVEVPA SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_199042 |
ORF Size | 630 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199042.2 |
RefSeq Size | 1027 bp |
RefSeq ORF | 633 bp |
Locus ID | 73754 |
UniProt ID | Q8CHW1 |
Gene Summary | DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR202256 | Thap1 (Myc-DDK-tagged) - Mouse THAP domain containing, apoptosis associated protein 1 (Thap1) |
CNY 2,400.00 |
|
MR202256L3 | Lenti ORF clone of Thap1 (Myc-DDK-tagged) - Mouse THAP domain containing, apoptosis associated protein 1 (Thap1) |
CNY 4,800.00 |
|
MR202256L4 | Lenti ORF clone of Thap1 (mGFP-tagged) - Mouse THAP domain containing, apoptosis associated protein 1 (Thap1) |
CNY 4,750.00 |