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Atg10 (BC023455) Mouse Tagged ORF Clone
CAT＃: MG202364
- TrueORF®
Atg10 (tGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268)
ORF Plasmid: DDK
"BC023455" in other vectors (4)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Agp10, APG10, Apg10p, Atg10l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202364 representing BC023455
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAGC AGATAGGCGATGGCTGGGAATGGAGAACAGCCAAGGAATGTTCTGATGGCTACATGTGCAAAACACAGTT TCGAATAAAGAATGAGGCCTCGACACCACATGTGGGAACACCTGCCAGTGTGCTCACATGTCTCCCTACA GAGGAGAATTTAGAGCTACCAATGGATGATTCTGAAGTGACGAGACCTGCAGCGGTGGCAGAAGTGATTA AACATGAGTATCATGTCTTATACTCTTGTAGTTACCAAGTGCCGGTTCTTTACTTTAGGGCAAGCTTTTT AGATGGGAGACCGTTAGCTCTGGAGGATATATGGGAAGGTGTTCATGAGTGCTATAAGCCGCGGCTGCTC CAGGGACCGTGGGATACCATCACCCAGCAGGAACATCCAATACTTGGGCAACCATTTTTTGTTCTACATC CCTGCAAGACAAATGAATTCATGACTGCTGTGCTAAAGAATTCACAGAAAATCAATAGAAATGTCAACTA TATCACATCATGGCTGAGCCTCGTGGGGCCAGTGGTTGGTCTGAATTTACCTCTGAGCTATGCTAAAGCA ACATCACAATCGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202364 representing BC023455
Red=Cloning site Green=Tags(s) MEDEFFGEKSFQHYCAEFIRHSQQIGDGWEWRTAKECSDGYMCKTQFRIKNEASTPHVGTPASVLTCLPT EENLELPMDDSEVTRPAAVAEVIKHEYHVLYSCSYQVPVLYFRASFLDGRPLALEDIWEGVHECYKPRLL QGPWDTITQQEHPILGQPFFVLHPCKTNEFMTAVLKNSQKINRNVNYITSWLSLVGPVVGLNLPLSYAKA TSQSE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023455 |
| ORF Size | 645 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023455, AAH23455 |
| RefSeq Size | 1153 bp |
| RefSeq ORF | 647 bp |
| Locus ID | 66795 |
| Gene Summary | E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206905 | Atg10 (untagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268), (10ug) |
CNY 3,230.00 |
|
| MR202364 | Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 2,400.00 |
|
| MR202364L3 | Lenti ORF clone of Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 4,750.00 |
|
| MR202364L4 | Lenti ORF clone of Atg10 (mGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 4,750.00 |
