Cldn5 (NM_013805) Mouse Tagged ORF Clone
CAT#: MG202442
- TrueORF®
Cldn5 (tGFP-tagged) - Mouse claudin 5 (Cldn5)
ORF Plasmid: DDK
"NM_013805" in other vectors (6)
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CNY 5,200.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI854493; MBEC1; Tmvc; Tmvcf |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202442 representing NM_013805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCTGCAGCGTTGGAAATTCTGGGTCTGGTGCTGTGTCTGGTAGGATGGGTGGGCTTGATCCTGG CGTGTGGGCTGCCCATGTGGCAGGTGACTGCCTTCCTGGACCACAACATCGTGACGGCGCAGACGACTTG GAAGGGGCTGTGGATGTCGTGCGTGGTGCAGAGTACCGGGCACATGCAGTGCAAGGTGTATGAATCTGTG CTGGCGCTGAGTGCGGAGGTGCAGGCAGCTCGGGCACTCACCGTGGGCGCTGTGCTGCTGGCGCTGGTGG CACTCTTTGTTACCTTGACCGGCGCTCAGTGCACCACCTGCGTGGCCCCGGGCCCAGTTAAGGCACGGGT AGCACTCACGGGAGGAGCGCTTTACGCGGTGTGCGGGCTGCTGGCACTCGTGCCGCTCTGCTGGTTCGCC AACATCGTTGTCCGCGAGTTCTATGATCCGACGGTGCCGGTGTCACAGAAGTACGAGCTGGGCGCGGCGC TGTACATCGGCTGGGCGGCCTCCGCACTGCTCATGTGCGGTGGCGGCCTCGTGTGTTGCGGCGCCTGGGT CTGCACCGGGCGCCCTGAGTTCAGCTTCCCGGTCAAGTACTCTGCGCCGCGGCGGCCCACGGCCAATGGC GATTACGACAAGAAGAACTATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202442 representing NM_013805
Red=Cloning site Green=Tags(s) MGSAALEILGLVLCLVGWVGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCVVQSTGHMQCKVYESV LALSAEVQAARALTVGAVLLALVALFVTLTGAQCTTCVAPGPVKARVALTGGALYAVCGLLALVPLCWFA NIVVREFYDPTVPVSQKYELGAALYIGWAASALLMCGGGLVCCGAWVCTGRPEFSFPVKYSAPRRPTANG DYDKKNYV TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013805 |
ORF Size | 654 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013805.4 |
RefSeq Size | 1415 bp |
RefSeq ORF | 657 bp |
Locus ID | 12741 |
UniProt ID | O54942 |
Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a critical component of endothelial tight junctions that control pericellular permeability. The knockout mice lacking this gene died within 10 h of birth and the blood-brain barrier in these mice against small molecules was selectively affected. This gene is expressed strongly in endothelium of normal lung and plays a regulation role during acrolein-induced acute lung injury. [provided by RefSeq, Aug 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Loss of CLDN5 in podocytes deregulates WIF1 to activate WNT signaling and contributes to kidney disease
,null,
Nature Communications
,PubMed ID 35332151
[Cldn5]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202457 | Cldn5 (untagged) - Mouse claudin 5 (Cldn5), (10ug) |
CNY 3,600.00 |
|
MR202442 | Cldn5 (Myc-DDK-tagged) - Mouse claudin 5 (Cldn5) |
CNY 3,600.00 |
|
MR202442L1 | Lenti ORF clone of Cldn5 (Myc-DDK-tagged) - Mouse claudin 5 (Cldn5) |
CNY 5,890.00 |
|
MR202442L2 | Lenti ORF clone of Cldn5 (mGFP-tagged) - Mouse claudin 5 (Cldn5) |
CNY 6,000.00 |
|
MR202442L3 | Lenti ORF clone of Cldn5 (Myc-DDK-tagged) - Mouse claudin 5 (Cldn5) |
CNY 5,890.00 |
|
MR202442L4 | Lenti ORF clone of Cldn5 (mGFP-tagged) - Mouse claudin 5 (Cldn5) |
CNY 6,000.00 |