Krtcap2 (BC043030) Mouse Tagged ORF Clone
CAT#: MG202511
- TrueORF®
Krtcap2 (tGFP-tagged) - Mouse keratinocyte associated protein 2 (cDNA clone MGC:57908 IMAGE:5693076)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0610010I12Rik; Kcp2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202511 representing BC043030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCAGCTTGTCCAGAGAGACAGCAGATGCCTTGGCTACAGAAGGCTGGAGACCAAGCAGGGCGGT GGCCTAGAATGCTCTTAGAAGCATTATTTCCTCCTTGCCACAAGAGGGCGCTGCACCCAAGGCGCGTAGA GGAAGAAGTAAAGCAAGTCCACGTTGGCCCCGACGCCTGCGTACAGACAACCGCGCCGGCTTTGGCTCCC CTCTTTCTCCAGGGACACGGATTGGACCACGGACGGGGCGTGATGGTAGTCGGTACCGGGACCTCCTTGG CGCTGTCCTCCCTCCTGTCCCTGCTGCTCTTCGCTGGCATGCAGATCTACAGCCGCCAGCTGGCCTCCAC CGAGTGGCTCACCATTCAGGGGGGCCTGCTGGGCTCCGGCCTTTTCGTCTTCTCCCTGACTGCCTTCAAT AATCTGGAGAATCTGGTCTTTGGTAAAGGCTTCCAAGCAAAAATCTTCCCTGAGATTCTCCTCTGCTTGC TGTTGGCGCTCTTTGCCTCCGGCCTCATCCACCGAGTTTGTGTCACCACTTGCTTCATCTTCTCCATGGT TGGTCTGTACTACATCAACAAGATATCGTCCACTCTGTACCAGGCAACAGCTCCAGTTCTCACACCGGCC AAAATCACTGGCAAGGGCAAGAAGAGAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202511 representing BC043030
Red=Cloning site Green=Tags(s) MSAACPERQQMPWLQKAGDQAGRWPRMLLEALFPPCHKRALHPRRVEEEVKQVHVGPDACVQTTAPALAP LFLQGHGLDHGRGVMVVGTGTSLALSSLLSLLLFAGMQIYSRQLASTEWLTIQGGLLGSGLFVFSLTAFN NLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLYQATAPVLTPA KITGKGKKRN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC043030 |
ORF Size | 662 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC043030, AAH43030 |
RefSeq Size | 1010 bp |
RefSeq ORF | 662 bp |
Locus ID | 66059 |
Gene Summary | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1.[UniProtKB/Swiss-Prot Function] |
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