Vamp7 (NM_011515) Mouse Tagged ORF Clone
CAT#: MG202515
- TrueORF®
Vamp7 (tGFP-tagged) - Mouse synaptobrevin like 1 (Sybl1)
ORF Plasmid: DDK
"NM_011515" in other vectors (4)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Sybl1; TI-VAMP; VAMP-7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202515 representing NM_011515
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATTCTTTTTGCTGTTGTTGCCAGGGGAACCACTATCCTTGCCAAACATGCTTGGTGTGGAGGAA ACTTCCTGGAGGTGACAGAGCAGATTCTGGCTAAGATACCTTCTGAAAATAATAAACTAACTTACTCACA TGGCAATTATTTGTTTCATTACATCTGCCAAGACAGGATTGTGTATCTTTGTATCACTGATGATGATTTT GAGCGTTCTCGAGCGTTCAGTTTTTTGAATGAAGTAAAGAAGAGATTCCAGACAACTTACGGTTCAAGAG CACAAACTGCACTTCCTTATGCTATGAATAGCGAGTTTTCAAGTGTTTTGGCTGCACAACTGAAGCATCA CTCTGAGAATAAGAGCCTAGACAAAGTGATGGAGACTCAAGCACAAGTGGATGAACTGAAAGGAATAATG GTCAGAAACATAGATTTAGTTGCTCAACGTGGAGAAAGGTTGGAATTGCTGATAGATAAAACAGAAAACC TTGTGGATTCATCTGTCACTTTCAAAACTACCAGCAGAAATCTTGCTCGAGCCATGTGTATGAAGAATAT CAAGCTCACTATCATCATCATCATTGTATCAATTGTGTTCATCTATATCATTGTGTCACTTCTCTGTGGT GGGTTTACATGGCCAAACTGTGTGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202515 representing NM_011515
Red=Cloning site Green=Tags(s) MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDRIVYLCITDDDF ERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIM VRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCG GFTWPNCVKK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011515 |
ORF Size | 660 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011515.5 |
RefSeq Size | 2534 bp |
RefSeq ORF | 663 bp |
Locus ID | 20955 |
UniProt ID | P70280 |
Gene Summary | Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200514 | Vamp7 (untagged) - Mouse vesicle-associated membrane protein 7 (Vamp7), (10ug) |
CNY 2,400.00 |
|
MR202515 | Vamp7 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 7 (Vamp7) |
CNY 2,400.00 |
|
MR202515L3 | Lenti ORF clone of Vamp7 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 7 (Vamp7) |
CNY 4,750.00 |
|
MR202515L4 | Lenti ORF clone of Vamp7 (mGFP-tagged) - Mouse vesicle-associated membrane protein 7 (Vamp7) |
CNY 4,750.00 |