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Znrf1 (NM_133206) Mouse Tagged ORF Clone
CAT＃: MG202714
- TrueORF®
Znrf1 (tGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1)
"NM_133206" in other vectors (6)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B830022L21Rik; nin283; Rnf42; Zrfp1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202714 representing NM_133206
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGGCAAGCAGAGCACGGCGGCCCGCTCTCGGGGCCCCTTCCCGGGGGTCTCTACGGATGACAGCG CCGTGCCGCCGCCGGGAGGGGCGCCCCACTTTGGGCACTACCGGACGGGCGGCGGGGCGATGGGGCTGCG CAGCCGCTCGGTCAGCTCGGTGGCGGGCATGGGCATGGACCCCAGCACGGCCGGAGGGGTGCCCTTTAGT CTCTACACCCCCGCCTCCCGCGGGACCGGCGACTCCGAGAGGGCGCCGGGCGGCGGAGGGTCCACGTCGG ACTCCACCTATGCCCACGGCAATGGTTACCAAGAGACCGGCGGCGGTCACCATAGAGACGGGATGCTGTA CCTGGGCTCCCGAGCCTCGCTGGCGGATGCTCTACCTCTGCACATCGCACCCAGGTGGTTCAGCTCGCAC AGTGGTTTCAAGTGCCCCATTTGTTCCAAGTCTGTGGCTTCCGATGAGATGGAAATGCACTTTATAATGT GTCTGAGCAAGCCTCGCCTGTCCTACAATGATGATGTGCTGACTAAAGATGCGGGTGAGTGTGTGATCTG CCTGGAGGAGCTGCTTCAGGGGGACACGATAGCCAGGCTGCCTTGCCTGTGCATCTATCACAAAAGCTGC ATAGACTCATGGTTTGAAGTGAACAGATCTTGTCCAGAGCACCCTGCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202714 representing NM_133206
Red=Cloning site Green=Tags(s) MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMDPSTAGGVPFS LYTPASRGTGDSERAPGGGGSTSDSTYAHGNGYQETGGGHHRDGMLYLGSRASLADALPLHIAPRWFSSH SGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSC IDSWFEVNRSCPEHPAD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133206 |
| ORF Size | 5559 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133206.4 |
| RefSeq Size | 3125 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 170737 |
| UniProt ID | Q91V17 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes Wallerian degeneration, a neurodegeneration disorder, by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200768 | Znrf1 (untagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
| MC218756 | Znrf1 (untagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1, (10ug) |
CNY 6,940.00 |
|
| MG216517 | Znrf1 (tGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1) transcript variant 1, (10ug) |
CNY 2,850.00 |
|
| MR216517 | Znrf1 (Myc-DDK-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 |
CNY 2,400.00 |
|
| MR216517L3 | Lenti ORF clone of Znrf1 (Myc-DDK-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 |
CNY 4,750.00 |
|
| MR216517L4 | Lenti ORF clone of Znrf1 (mGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 |
CNY 4,750.00 |
