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Chpt1 (BC031435) Mouse Tagged ORF Clone
CAT＃: MG202889
- TrueORF®
Chpt1 (tGFP-tagged) - Mouse choline phosphotransferase 1 (cDNA clone MGC:25905 IMAGE:4221062)
ORF Plasmid: DDK
"BC031435" in other vectors (4)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC28885 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202889 representing BC031435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGCGCCGGGGCCAGGCCGGCGCCGCGCTGGGTAAAGGCGCTAGGTGAGCCGCTCAGTGCCG CGCAGCTGCGGCGGCTGGAGGAGCACCGCTACACCGCGGTGGGAGAGTCGCTGTTCGAGCCGCCGCTGCA GCTTTACTGGACCTGGCTGCTCCAATGGATCCCGCTCTGGATGGCCCCCAACACCATCACCCTCATCGGC CTCGCCATCAACCTGGTCACCACACTAGTGCTCATCTTCTACTGCCCTACAGTCACGGAGGAGGCACCAT ACTGGACATACCTTTTATGTGCCCTGGGACTCTTTATCTACCAGTCACTGGATGCCATTGATGGGAAACA AGCCAGAAGGACAAACTCTTGCTCTCCCTTAGGGGAACTATTTGATCATGGTTGTGACTCTCTTTCCACA GTATTTATGGCCATCGGCGCTTCCATTGCTGTTCGCCTAGGAACACATCCTGACTGGTTGTTTTTCTGCT CTTTCGTTGGGATGTTCATGTTTTACTGTGCTCATTGGCAGACTTACGTCTCAGGAGTGTTAAGATTTGG AAGTCAAGAAGTTTTCTTCCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGATCACAACCATCTGTAACAAGATCTGACGCCCTCT TCTGGAGTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202889 representing BC031435
Red=Cloning site Green=Tags(s) MAAGAGARPAPRWVKALGEPLSAAQLRRLEEHRYTAVGESLFEPPLQLYWTWLLQWIPLWMAPNTITLIG LAINLVTTLVLIFYCPTVTEEAPYWTYLLCALGLFIYQSLDAIDGKQARRTNSCSPLGELFDHGCDSLST VFMAIGASIAVRLGTHPDWLFFCSFVGMFMFYCAHWQTYVSGVLRFGSQEVFFRAGEMAQWVRAPDCSSE GPEFKSQQPHGGSQPSVTRSDALFWSV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031435 |
| ORF Size | 713 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031435, AAH31435 |
| RefSeq Size | 843 bp |
| RefSeq ORF | 713 bp |
| Locus ID | 212862 |
| Gene Summary | Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218562 | Chpt1 (untagged) - Mouse choline phosphotransferase 1 (cDNA clone MGC:25905 IMAGE:4221062), (10ug) |
CNY 6,940.00 |
|
| MR202889 | Chpt1 (Myc-DDK-tagged) - Mouse choline phosphotransferase 1 (cDNA clone MGC:25905 IMAGE:4221062) |
CNY 2,380.00 |
|
| MR202889L3 | Lenti ORF clone of Chpt1 (Myc-DDK-tagged) - Mouse choline phosphotransferase 1 (cDNA clone MGC:25905 IMAGE:4221062) |
CNY 4,280.00 |
|
| MR202889L4 | Lenti ORF clone of Chpt1 (mGFP-tagged) - Mouse choline phosphotransferase 1 (cDNA clone MGC:25905 IMAGE:4221062) |
CNY 4,280.00 |
