Mrps27 (BC049149) Mouse Tagged ORF Clone

CAT#: MG203136

  • TrueORF®

Mrps27 (tGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524)

ORF Plasmid: DDK tGFP



  "BC049149" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2610028H14Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203136 representing BC049149
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCAAGAAGCCTTTGACGTGCCGTCCACCCAGTTTCTCTCCCTCTATGTTTTATACCGTTGCCTAG
CAGAGAAGACGGAGCTCACATGGGAAGAAGAAAGAGACTTTGGGGCGTCCCTCTTGCTTTCTGGCCTGAA
ACAAAGGAACACAGTGGGCCTGAGTTCCCAGCTGTATGGCTATGCACTTCTCGGGAAGGTGGAACTGCAG
CGTGGGGTGCGGGCTGTATACCACGGCATGCCTCTGATGTGGACACCAGGCTACCTCGACAGAGCCCTCC
AAGTGATGGAGAGGGTGGCCTCCTCCCCTGAAGACCTCAAGCTGGGTAGAGAAGTGCTCGATGTGCTGGA
TGGAGTCCTGAAGGTGGTGACGTCTCCAGATGTCCAGACTTCAGAGGCACAGCCTCAGGAAGGTGAAGAT
GGCTTGGGCTCAGCAAACCTGGTGGAGCAGCTAGACACTGAGGAACCAGAACAGTCCAAGCTTCCCCGAT
ACCTCGAGCGCTTTCAGGCCTCTCGGTCTAAACTGCAGGAGCTGAACAGAGTTGAGTCTGAAAGTCTTTT
GACTCTGACCACCCAGCTTGTAAAAGAAAAACTCCCCGCCTGTGAGGCCGAGGACCTGGCCACCTACGAG
CAGAAACTCCGTGAGTGGCACCTGGAGCGGGTGCAGTTGATCCAGAGGGAGCAGGAACAGAGGGAGAAGG
CCAAGCAAGAGTACCAGGCGCTGAGTGCGGCAGAGAAAGCAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203136 representing BC049149
Red=Cloning site Green=Tags(s)

MMQEAFDVPSTQFLSLYVLYRCLAEKTELTWEEERDFGASLLLSGLKQRNTVGLSSQLYGYALLGKVELQ
RGVRAVYHGMPLMWTPGYLDRALQVMERVASSPEDLKLGREVLDVLDGVLKVVTSPDVQTSEAQPQEGED
GLGSANLVEQLDTEEPEQSKLPRYLERFQASRSKLQELNRVESESLLTLTTQLVKEKLPACEAEDLATYE
QKLREWHLERVQLIQREQEQREKAKQEYQALSAAEKAA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC049149
ORF Size 746 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC049149, AAH49149
RefSeq Size 1833 bp
RefSeq ORF 746 bp
Locus ID 218506
Gene Summary RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...