Derl1 (NM_024207) Mouse Tagged ORF Clone
CAT#: MG203204
- TrueORF®
Derl1 (tGFP-tagged) - Mouse Der1-like domain family, member 1 (Derl1)
ORF Plasmid: DDK
"NM_024207" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110021N07Rik; AI195141; AW551338; Derlin-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203204 representing NM_024207
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACATCGGGGACTGGTTCAGGAGCATCCCGGCCATCACGCGCTACTGGTTTGCTGCCACCGTTG CTGTCCCCTTGATCGGCAAGCTCGGCATCATCAGCCCGGCCTACTTCTTCCTCTGGCCCGAAGCCTTCCT CTATCGCTTCCAGATATGGAGGCCGTTCACTGCCACCTTTTACTTCCCCGTGGGCCCAGGGACTGGATTT CTGTATTTGGTCAATTTATATTTCTTATATCAGTATTCTACTCGGCTTGAAGCAGGAGCTTTTGACGGGA GGCCAGCAGACTATTTATTCATGCTTCTCTTTAACTGGATCTGCATCGTTATTACTGGCTTAGCAATGGA TATGCAGCTGCTGATGATCCCTCTGATCATGTCAGTGCTCTACGTCTGGGCCCAGCTGAACAGAGACCTG ATCGTGTCGTTCTGGTTCGGAACGCGATTTAAGGCCTGTTACTTACCTTGGGTTATCCTTGGATTCAACT ATATCATTGGAGGCTCGGTGATCAATGAGCTCATTGGAAACCTTGTCGGCCATCTTTATTTCTTCCTGAT GTTCAGATACCCAATGGACTTGGGAGGAAGGAATTTTCTGTCCACACCTCAGTTTTTGTACCGCTGGCTA CCCAGTAGGAGAGGAGGGGTGTCAGGATTTGGTGTGCCCCCTGCTAGCATGAGGCGAGCTGCTGATCAGA ATGGCGGAGGCGGGAGACACAACTGGGGCCAGGGCTTCCGACTTGGAGACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203204 representing NM_024207
Red=Cloning site Green=Tags(s) MSDIGDWFRSIPAITRYWFAATVAVPLIGKLGIISPAYFFLWPEAFLYRFQIWRPFTATFYFPVGPGTGF LYLVNLYFLYQYSTRLEAGAFDGRPADYLFMLLFNWICIVITGLAMDMQLLMIPLIMSVLYVWAQLNRDL IVSFWFGTRFKACYLPWVILGFNYIIGGSVINELIGNLVGHLYFFLMFRYPMDLGGRNFLSTPQFLYRWL PSRRGGVSGFGVPPASMRRAADQNGGGGRHNWGQGFRLGDQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024207 |
ORF Size | 753 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024207.4 |
RefSeq Size | 3146 bp |
RefSeq ORF | 756 bp |
Locus ID | 67819 |
UniProt ID | Q99J56 |
Gene Summary | Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the misfolded protein. Also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200453 | Derl1 (untagged) - Mouse Der1-like domain family, member 1 (Derl1), (10ug) |
CNY 2,400.00 |
|
MR203204 | Derl1 (Myc-DDK-tagged) - Mouse Der1-like domain family, member 1 (Derl1) |
CNY 2,400.00 |
|
MR203204L3 | Lenti ORF clone of Derl1 (Myc-DDK-tagged) - Mouse Der1-like domain family, member 1 (Derl1) |
CNY 4,750.00 |
|
MR203204L4 | Lenti ORF clone of Derl1 (mGFP-tagged) - Mouse Der1-like domain family, member 1 (Derl1) |
CNY 4,750.00 |