Snf8 (NM_033568) Mouse Tagged ORF Clone
CAT#: MG203337
- TrueORF®
Snf8 (tGFP-tagged) - Mouse SNF8, ESCRT-II complex subunit, homolog (S
ORF Plasmid: DDK
"NM_033568" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D11Moh34 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203337 representing NM_033568
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCGGCGCGGGGTGGGAGCTGGCGCCATTGCCAAGAAGAAACTCGCAGAGGCCAAGTATAAGGAGC GAGGGACTGTCCTGGCTGAGGACCAGCTGGCCCAGATGTCAAAACAGCTGGACATGTTCAAGACCAACCT AGAAGAATTTGCCAGCAAGCACAAGCAAGAGATCCGGAAGAATCCTGAGTTCCGAGTCCAGTTCCAAGAC ATGTGTGCAACCATTGGGGTGGATCCCCTGGCCTCTGGAAAAGGCTTTTGGTCTGAGATGCTGGGCGTTG GGGACTTCTATTATGAACTGGGTGTCCAGATTATTGAAGTGTGCCTGGCCCTCAAACATCGGAATGGAGG TCTGATAACTCTGGAGGAGCTACATCAGCAGGTGTTAAAAGGAAGGGGCAAGTTTGCTCAGGATGTCAGC CAAGACGACCTGATCAGGGCCATCAAGAAGCTGAAAGCCCTGGGCACTGGATTCGGCATCATCCCTGTGG GAGGCACTTACCTCATCCAGTCTGTTCCCGCTGAGCTCAATATGGATCACACTGTTGTGCTGCAGCTGGC CGAGAAAAACGGGTATGTGACTGTCAGTGAAATCAAAACCAGTCTTAAATGGGAGACGGAGCGAGCACGG CAAGTGCTGGAACACCTGCTGAAGGAAGGACTGGCCTGGCTGGATCTGCAGGCTCCAGGGGAGGCCCACT ACTGGCTGCCAGCTCTTTTCACAGATCTCTACTCCCAGGAGATATCAGCTGAGGAGGCCAAAGAAGCCTT CCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203337 representing NM_033568
Red=Cloning site Green=Tags(s) MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQD MCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVS QDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKTSLKWETERAR QVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEISAEEAKEAFP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_033568 |
ORF Size | 774 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033568.3 |
RefSeq Size | 949 bp |
RefSeq ORF | 777 bp |
Locus ID | 27681 |
UniProt ID | Q9CZ28 |
Gene Summary | Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207474 | Snf8 (untagged) - Mouse SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (Snf8), (10ug) |
CNY 3,990.00 |
|
MR203337 | Snf8 (Myc-DDK-tagged) - Mouse SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (Snf8) |
CNY 2,400.00 |
|
MR203337L3 | Lenti ORF clone of Snf8 (Myc-DDK-tagged) - Mouse SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (Snf8) |
CNY 4,750.00 |
|
MR203337L4 | Lenti ORF clone of Snf8 (mGFP-tagged) - Mouse SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (Snf8) |
CNY 4,750.00 |