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Trdn (BC034343) Mouse Tagged ORF Clone
CAT＃: MG203634
- TrueORF®
Trdn (tGFP-tagged) - Mouse triadin (cDNA clone MGC:8119 IMAGE:3589077)
ORF Plasmid: DDK
"BC034343" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TDN, triadin-1, triadin-2, triadin-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203634 representing BC034343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAGATCACTGCTGAAGGAAATGCATCGACAACCACAACGGTGATAGACAACAAAAATGGATCTA TTCCTAAATCCCCTGGAAAGGTGCTGAAGAGGTCTGTCACCGAAGACATTGTGACAACATTCAGCTCCCC TGCAGCCTGGCTTCTTGTCATCGCTCTGATTATCACATGGTCAGCTGTTGCTATCGTGATGTTTGATTTA GTGGATTATAAAAACTTTTCAGCAAGCTCCATTGCCAAGATTGGCTCAGATCCTCTAAAACTAGTGAATG ATGCTGTGGAGGAGACAACAGACTGGATCTACGGCTTCTTCTCTTTGCTATCTGACATCATCTCATCTGA AGGTGACGAAGATGATGAGGATGCAGATGAAGACATTGATAAAGGAGAAATAGAAGAACCTCCCTTAAAA AGAAAAGAAATACACCAAGAAAAGGCTGAAAAAGAGGAGAAACCTGAGAAGAAAATACAAACTAAAGCTT CACACAGAGAAAAGGAGAAAGGAAAAGAAAAATTAAAAGGAGAAAAACCTGAGAAGACAGCAACTCACAA AGAGAAACTTGAGAAAAAAGAAAGACCAGAGACGAAGATGATGGCAAAAGAGGACAAGAAAATTAAGACT AAGGAAAAGACTGAAGAAAAGGCTAAGAAGGAAATGAAAGTTGGAAAACAGGAGAAAGTGAAACCAACAG CTGCAAAGCCAAAGAAACTCCGAAAACACCACCAAAGGCCAGAAAGAAGGATGACAAAGAGATGCCAGCT GTGCATGAGCAGAAAGGACAAAGCCCAGTTATGCCACCACCATCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203634 representing BC034343
Red=Cloning site Green=Tags(s) MTEITAEGNASTTTTVIDNKNGSIPKSPGKVLKRSVTEDIVTTFSSPAAWLLVIALIITWSAVAIVMFDL VDYKNFSASSIAKIGSDPLKLVNDAVEETTDWIYGFFSLLSDIISSEGDEDDEDADEDIDKGEIEEPPLK RKEIHQEKAEKEEKPEKKIQTKASHREKEKGKEKLKGEKPEKTATHKEKLEKKERPETKMMAKEDKKIKT KEKTEEKAKKEMKVGKQEKVKPTAAKPKKLRKHHQRPERRMTKRCQLCMSRKDKAQLCHHHH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034343 |
| ORF Size | 816 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034343.1 |
| RefSeq Size | 1045 bp |
| RefSeq ORF | 818 bp |
| Locus ID | 76757 |
| Gene Summary | Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength (PubMed:19843516). Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218487 | Trdn (untagged) - Mouse triadin (cDNA clone MGC:8119 IMAGE:3589077), (10ug) |
CNY 6,940.00 |
|
| MR203634 | Trdn (Myc-DDK-tagged) - Mouse triadin (cDNA clone MGC:8119 IMAGE:3589077) |
CNY 3,840.00 |
|
| MR203634L3 | Lenti ORF clone of Trdn (Myc-DDK-tagged) - Mouse triadin (cDNA clone MGC:8119 IMAGE:3589077) |
CNY 5,890.00 |
|
| MR203634L4 | Lenti ORF clone of Trdn (mGFP-tagged) - Mouse triadin (cDNA clone MGC:8119 IMAGE:3589077) |
CNY 5,890.00 |
